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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
01/06/2020 |
Data da última atualização: |
29/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RAMOS-GONZÁLEZ, P. L.; COSTA-RODRIGUES, M. da; POTSCLAM-BARRO, M.; CHABI-JESUS, C.; BANGUELA-CASTILLO, A.; HARAKAVA, RI.; KITAJIMA, E. W.; ASTUA, J. de F. |
Afiliação: |
PEDRO LUIS RAMOS-GONZÁLEZ, Instituto Biológico; MARIANE DA COSTA-RODRIGUES, Instituto Biológico; MATHEUS POTSCLAM-BARRO, Instituto Biológico; CAMILA CHABI-JESUS, Instituto Biológico; ALEXANDER BANGUELA-CASTILLO, Instituto Biológico; RICARDO HARAKAVA, Instituto Biológico; ELLIOT W. KITAJIMA, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
First genome sequence of an isolate of hibiscus chlorotic ringspotvirus from the Western hemisphere. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Tropical Plant Pathology, 2020. |
Idioma: |
Inglês |
Conteúdo: |
For the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis (L.) plant collected in São Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38 ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed. |
Thesagro: |
Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01689naa a2200217 a 4500 001 2122808 005 2021-11-29 008 2020 bl uuuu u00u1 u #d 100 1 $aRAMOS-GONZÁLEZ, P. L. 245 $aFirst genome sequence of an isolate of hibiscus chlorotic ringspotvirus from the Western hemisphere.$h[electronic resource] 260 $c2020 520 $aFor the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis (L.) plant collected in São Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38 ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed. 650 $aVírus 700 1 $aCOSTA-RODRIGUES, M. da 700 1 $aPOTSCLAM-BARRO, M. 700 1 $aCHABI-JESUS, C. 700 1 $aBANGUELA-CASTILLO, A. 700 1 $aHARAKAVA, RI. 700 1 $aKITAJIMA, E. W. 700 1 $aASTUA, J. de F. 773 $tTropical Plant Pathology, 2020.
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1. |  | SANTOS, B. H. C. dos; RIBEIRO, R. C. F.; OLIVEIRA, R. M.; XAVIER, A. A.; ROCHA, L. DE S.; SANTOS NETO, J. A. dos; DIAS-ARIEIRA, C. R.; MIZOBUTSI, E. H. Silicato de cálcio e magnésio no controle de Meloidogyne Javanica em pepineiro em diferentes texturas de solo. Revista Brasileira de Agropecuária Sustentável, v.8, n.1, p.104-109, Mar., 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
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