Data/hora: 14/06/2024 07:44:46

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	19/09/2018	
Data da última atualização: 	28/09/2018	
Autoria: 	SILVA, L. L.; PESTANA, K. N.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de.	
Afiliação: 	LEANDRO L. SILVA, UFRB; KÁTIA N. PESTANA; CLAUDIA FORTES FERREIRA, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF.	
Título: 	Differentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Tropical Plant Pathology, v.43, p.194-20, 2018.	
ISSN: 	1983-2052	
Idioma: 	Inglês	
Conteúdo: 	Different species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing.	
Thesagro: 	Mandioca.	
Thesaurus Nal: 	Cassava.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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