Data/hora: 14/06/2024 06:40:48

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 1
Data corrente: | 06/10/2010
Data da última atualização: | 22/02/2011
Autoria: | SOUZA, F. V. D.; CANTO, A. M. M. E.; SOUZA, A. da S.; COSTA, M. A. P. de C.
Afiliação: | FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; Ana Maria Mascarenhas Eloy Canto, UFRB; ANTONIO DA SILVA SOUZA, CNPMF; Maria Angélica Pereira de Carvalho Costa, UFRB.
Título: | Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants.
Ano de publicação: | 2010
Fonte/Imprenta: | Revista Brasileira de Fruticultura, Jaboticabal, v. 32, n. 2, p. 612-617, jun. 2010.
ISSN: | 0100-2945
Idioma: | Inglês
Notas: | Disponível em:<http://www.scielo.br/pdf/rbf/v32n2/aop07910.pdf>. Acesso em 28 out.2010.
Conteúdo: | This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC ± 1oC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation treatment, were cultivated in proliferation medium and the number of regenerated plants per treatment was evaluated at 60 days of cultivation. The treatment that promoted the best etiolation of plants also promoted the worst regeneration rates, demonstrating the residual effect of the auxin used in the previous step in the regeneration of plants of the pineapple hybrid evaluated.
Palavras-Chave: | In vitro; Nodal segments; Plant regulators.
Thesagro: | Germoplasma; Melhoramento Genético Vegetal; Micropropagação.
Thesaurus Nal: | Ananas comosus var. comosus; germplasm conservation; micropropagation.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/23363/1/aop08410id26912RBF.pdf
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (26913-1) - UPC - AP - PP -  -  -  - PCT