| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
28/08/1995 |
|
Data da última atualização: |
17/05/2023 |
|
Autoria: |
KOBAYASHI, S.; IKEDA, I.; UCHIMIYA, H. |
|
Afiliação: |
SHOZO KOBAYASHI, University of Tsukuba; ISAMI IKEDA; HIROFUMI UCHIMIYA. |
|
Título: |
Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts. |
|
Ano de publicação: |
1985 |
|
Fonte/Imprenta: |
Plant Cell Tissue Organ Culture. v.4, p.249-259, 1985. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Using `Trovita' orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (~4 x 10sobre4/ml) and low mannitol concentrations (~ 0.4 M). Two altenatives to achieve high frequency embryogenesis (~ 70%) were to either dilute the cells to lower densities, or to do serial transfers of cells to fresh medium. Orange protoplasts (cells) showed embryogenic potential, and repression of embryo-genesis occurred when propoplasts were cultured at a high density and/or under high osmotic pressure. |
|
Palavras-Chave: |
Citrus sinensis Osb; Inhibitor (s); Nucellar callus; plant regeneration; Protoplast. |
|
Thesaurus Nal: |
somatic embryogenesis. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01379naa a2200217 a 4500
001 1646877
005 2023-05-17
008 1985 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, S.
245 $aConditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts.$h[electronic resource]
260 $c1985
520 $aUsing `Trovita' orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (~4 x 10sobre4/ml) and low mannitol concentrations (~ 0.4 M). Two altenatives to achieve high frequency embryogenesis (~ 70%) were to either dilute the cells to lower densities, or to do serial transfers of cells to fresh medium. Orange protoplasts (cells) showed embryogenic potential, and repression of embryo-genesis occurred when propoplasts were cultured at a high density and/or under high osmotic pressure.
650 $asomatic embryogenesis
653 $aCitrus sinensis Osb
653 $aInhibitor (s)
653 $aNucellar callus
653 $aplant regeneration
653 $aProtoplast
700 1 $aIKEDA, I.
700 1 $aUCHIMIYA, H.
773 $tPlant Cell Tissue Organ Culture.$gv.4, p.249-259, 1985.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | RIOS, S. de A.; DIAS, M. S. C.; CORDEIRO, Z. J. M.; SOUZA, W. M. de; SILVA, J. J. C.; BARBOSA, J. A. A.; PINHO, R. S. C. de; ABREU, S. C.; SANTOS, L. O. Sistema de pré-aviso para controle de Sigatoka-amarela no norte de Minas Gerais. Biotemas, v. 26, n. 3, p. 109-115, set. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Mandioca e Fruticultura. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
