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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/07/2009 |
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Data da última atualização: |
29/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIE, J. V.; GONZÁLEZ VERDEJO, C. I.; DITA, M. A.; SALVADOR, N.; ROMAN, B. |
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Afiliação: |
José Vicente Die Ramón, IFAPA; Clara Isabel González-Verdejo, IFAPA; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; Nadal Moyano Salvador, IFAPA; Belén Román, IFAPA. |
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Título: |
Gene expression analysis of molecular mechanisms of defense induced in Medicago truncatula parasitized by Orobanche crenata. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, Paris, v. 47, n. 3, p. 635-641, mar. 2009. |
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ISSN: |
0981-9428 |
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DOI: |
DOI:10.1016/j.plaphy.2009.02.016 |
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Idioma: |
Inglês |
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Conteúdo: |
The infection of Medicago truncatula Gaertn. roots with the obligate parasite Orobanche crenata Forsk. is a useful model for studying the molecular events involved in the legumes-parasite interaction. In order to gain insight into the identification of gene-regulatory elements involved in the resistance mechanism, the temporal expression pattern of ten defense-related genes was carried out using real-time quantitative reverse-transcription polymerase chain reaction assays. The induction of all of the analyzed transcripts significantly increased over a range from 2- to 321-fold higher than the control depending on the gene and time point. The transcriptional changes observed in response to O. crenata infection suggest that resistance could rely on both, the induction of general defense-related genes and more specific responses. |
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Palavras-Chave: |
Real-time RT-PCR. |
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Thesaurus Nal: |
gene expression; Medicago truncatula; Orobanche crenata. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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024 7 $aDOI:10.1016/j.plaphy.2009.02.016$2DOI
100 1 $aDIE, J. V.
245 $aGene expression analysis of molecular mechanisms of defense induced in Medicago truncatula parasitized by Orobanche crenata.$h[electronic resource]
260 $c2009
520 $aThe infection of Medicago truncatula Gaertn. roots with the obligate parasite Orobanche crenata Forsk. is a useful model for studying the molecular events involved in the legumes-parasite interaction. In order to gain insight into the identification of gene-regulatory elements involved in the resistance mechanism, the temporal expression pattern of ten defense-related genes was carried out using real-time quantitative reverse-transcription polymerase chain reaction assays. The induction of all of the analyzed transcripts significantly increased over a range from 2- to 321-fold higher than the control depending on the gene and time point. The transcriptional changes observed in response to O. crenata infection suggest that resistance could rely on both, the induction of general defense-related genes and more specific responses.
650 $agene expression
650 $aMedicago truncatula
650 $aOrobanche crenata
653 $aReal-time RT-PCR
700 1 $aGONZÁLEZ VERDEJO, C. I.
700 1 $aDITA, M. A.
700 1 $aSALVADOR, N.
700 1 $aROMAN, B.
773 $tPlant Physiology and Biochemistry, Paris$gv. 47, n. 3, p. 635-641, mar. 2009.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | SANTOS-SEREJO, J. A. dos; BARBOSA, A. M. M.; LUNA, J. V. U. Tamarindo. In: SANTOS-SEREJO, J. A. dos; DANTAS, J. L. L.; SAMPAIO, C. V.; COELHO, Y. da S. (Ed.). Fruticultura tropical: espécies regionais e exóticas. Brasília, DF: Embrapa Informação Tecnológica, 2009. p. 437-455.| Tipo: Capítulo em Livro Técnico-Científico |
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