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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Semiárido. |
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Data corrente: |
28/09/2018 |
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Data da última atualização: |
03/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, L. M. B.; ALONSO, J. D.; KIILL, C. P.; FERREIRA, N. N.; BUZZÁ, H. H.; GODOI, D. R. M. de; BRITTO, D. de; ASSIS, O. B. G. de; SERAPHIM, T. V.; BORGES, J. C.; GREMIÃO, M. P. D. |
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Afiliação: |
DOUGLAS DE BRITTO, CPATSA; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA. |
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Título: |
Exploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
European Polymer Journal, v. 103, p. 238-250, jun. 2018. |
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Páginas: |
238-250 |
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DOI: |
10.1016/j.eurpolymj.2018.04.013 |
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Idioma: |
Inglês |
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Conteúdo: |
Monoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers. MenosMonoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited di@... Mostrar Tudo |
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Palavras-Chave: |
Bevacizumab; Drug delivery platform; Interações supramoleculares; Mucoadesão; Mucoadhesion; Nanoparticula; Nanopartículas poliméricas; Penetração do muco; Plataforma de entrega de medicamentos; Polymeric nanoparticles; Supramolecular interactions. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03262naa a2200397 a 4500
001 2096760
005 2018-10-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.eurpolymj.2018.04.013$2DOI
100 1 $aFERREIRA, L. M. B.
245 $aExploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery.$h[electronic resource]
260 $c2018
300 $a238-250
520 $aMonoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-specific targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to specific purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited different be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating effect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive effect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers.
653 $aBevacizumab
653 $aDrug delivery platform
653 $aInterações supramoleculares
653 $aMucoadesão
653 $aMucoadhesion
653 $aNanoparticula
653 $aNanopartículas poliméricas
653 $aPenetração do muco
653 $aPlataforma de entrega de medicamentos
653 $aPolymeric nanoparticles
653 $aSupramolecular interactions
700 1 $aALONSO, J. D.
700 1 $aKIILL, C. P.
700 1 $aFERREIRA, N. N.
700 1 $aBUZZÁ, H. H.
700 1 $aGODOI, D. R. M. de
700 1 $aBRITTO, D. de
700 1 $aASSIS, O. B. G. de
700 1 $aSERAPHIM, T. V.
700 1 $aBORGES, J. C.
700 1 $aGREMIÃO, M. P. D.
773 $tEuropean Polymer Journal$gv. 103, p. 238-250, jun. 2018.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/06/2010 |
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Data da última atualização: |
06/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVEIRA, M. C. T. da; NASCIMENTO JÚNIOR, D. do; SILVA, S. C. da; EUCLIDES, V. P. B.; MONTAGNER, D. B.; SBRISSIA, A. F.; RODRIGUES. C. S.; SOUSA, B. M. de L.; PENA, K. da S.; VILELA, H. H. |
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Afiliação: |
MÁRCIA CRISTINA TEIXEIRA DA SILVEIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; DOMICIO DO NASCIMENTO JÚNIOR, UNIVERSIDADE FEDERAL DE VIÇOSA; SILA CARNEIRO DA SILVA, ESCOLA SUPERIOR DE AGRICULTURA "LUIZ DE QUEIROZ"; VALERIA PACHECO BATISTA EUCLIDES, CNPGC; DENISE BAPTAGLIN MONTAGNER, CNPGC; ANDRÉ FISCHER SBRISSIA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; CARLINDO SANTOS RODRIGUES, UNIVERSIDADE FEDERAL DE VIÇOSA; BRÁULIO MAIA DE LANA SOUSA, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINE DA SILVA PENA, UNIVERSIDADE FEDERAL DE VIÇOSA; HÉLIO HENRIQUE VILELA, UNIVERSIDADE FEDERAL DE VIÇOSA. |
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Título: |
Morphogenetic and structural comparative characterization of tropical forage grass cultivars under free growth. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Scientia Agricola, v. 67, n. 2, p. 136-142, Mar./Abr. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - Understanding of the morphogenetic characteristics allows comprehension of aspects related to the form and function of forage plants, providing opportunities to identify potentially high productive plants and distinct defoliation management requirements. The objective of this experiment was to carry out a comparative study of ten tropical forage grasses using morphogenetic and structural variables. Treatments corresponded to grasses of the Panicum genus (P. maximum cultivars Tanzânia and Mombaça) and the Brachiaria genus (B. brizantha cultivars Piatã, Marandu, Xaraés, Capiporã and Arapoty; B. decumbens cultivar Basilisk; B. humidicola cultivars Comum and Tupi), evaluated under free growth conditions.. Response variables were leaf appearance and elongation rates, phyllochron, stem elongation rate, final leaf length, number of live leaves per tiller, leaf life span and tiller appearance, death and survival rates. There was difference between cultivars for these variables, indicating a large variability within plants. Considering the morphogenetic and structural variables of leaves and tillers, B. brizantha had a similar pattern of variation to those of P. maximum, and B. Decumbens, similar to those of B. humidicola. Tiller appearance was large at the onset of the experiment and second and third generations comprised the large majority of tiller population for the cultivars Mombaça, Tanzânia, Xaraés and Capiporã. Group analysis based on plant morphogenetic and structural characteristics of leaves and tillers was an interesting way of grouping cultivars, indicating the importance and the potential of using morphogenesis in plant selection and evaluation programmes. RESUMO - O estudo das características morfogênicas permite compreender aspectos relativos à forma e função das plantas forrageiras além de possibilitar identificar plantas com potenciais produtivos e exigências de manejo diferentes. O objetivo deste trabalho foi avaliar, comparativamente, dez plantas forrageiras tropicais por meio de variáveis morfogênicas e estruturais. Os tratamentos corresponderam a cultivares pertencentes ao gênero Panicum (P. maximum cvs. Tanzânia e Mombaça) e ao gênero Brachiaria (B. brizantha cvs. Piatã, Marandu, Xaraés, Capiporã, e Arapoty; B. decumbens cv. Basilisk; B. humidicola cvs. Comum e Tupi), avaliados sob condições de crescimento livre. Foram avaliadas a taxa de aparecimento e alongamento de folhas, filocrono, taxa de alongamento de colmos, comprimento final da lâmina foliar, número de folhas vivas por perfilho, duração de vida das folhas e padrão demográfico de perfilhamento. Houve diferença entre cultivares para as variáveis, indicando grande variabilidade no material estudado. Considerando as características morfogênicas e estruturais de folhas e perfilhos, as B. brizantha apresentaram comportamento parecido com os P. maximum, e B. decumbens mais próximo das B. humidicola. Verificou-se que o aparecimento de perfilhos foi inicialmente elevado e que a segunda e, ou, terceira gerações contribuíram de forma significativa para o número total de perfilhos dos cultivares Mombaça, Tanzânia, Xaraés e Capiporã. A análise de agrupamento baseada nas características morfogênicas e estruturais de folhas e perfilhos foi um método interessante para agrupar cultivares, demonstrando a importância e o potencial de uso da morfogênese em trabalhos de seleção e avaliação de plantas forrageiras. MenosABSTRACT - Understanding of the morphogenetic characteristics allows comprehension of aspects related to the form and function of forage plants, providing opportunities to identify potentially high productive plants and distinct defoliation management requirements. The objective of this experiment was to carry out a comparative study of ten tropical forage grasses using morphogenetic and structural variables. Treatments corresponded to grasses of the Panicum genus (P. maximum cultivars Tanzânia and Mombaça) and the Brachiaria genus (B. brizantha cultivars Piatã, Marandu, Xaraés, Capiporã and Arapoty; B. decumbens cultivar Basilisk; B. humidicola cultivars Comum and Tupi), evaluated under free growth conditions.. Response variables were leaf appearance and elongation rates, phyllochron, stem elongation rate, final leaf length, number of live leaves per tiller, leaf life span and tiller appearance, death and survival rates. There was difference between cultivars for these variables, indicating a large variability within plants. Considering the morphogenetic and structural variables of leaves and tillers, B. brizantha had a similar pattern of variation to those of P. maximum, and B. Decumbens, similar to those of B. humidicola. Tiller appearance was large at the onset of the experiment and second and third generations comprised the large majority of tiller population for the cultivars Mombaça, Tanzânia, Xaraés and Capiporã. Group analysis based on plant morphogenetic and struct... Mostrar Tudo |
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Palavras-Chave: |
Braquiária. |
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Thesagro: |
Brachiaria Brizantha; Brachiaria Decumbens; Brachiaria Humidicola; Gramínea Forrageira; Morfogênese; Panicum Maximum; Pastagem; Planta Forrageira. |
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Thesaurus NAL: |
Forage grasses; Morphogenesis; Panicum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/855795/1/Morphogenetic-structural-comparative-2010.pdf
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Marc: |
LEADER 04582naa a2200373 a 4500
001 1855795
005 2023-06-06
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, M. C. T. da
245 $aMorphogenetic and structural comparative characterization of tropical forage grass cultivars under free growth.
260 $c2010
520 $aABSTRACT - Understanding of the morphogenetic characteristics allows comprehension of aspects related to the form and function of forage plants, providing opportunities to identify potentially high productive plants and distinct defoliation management requirements. The objective of this experiment was to carry out a comparative study of ten tropical forage grasses using morphogenetic and structural variables. Treatments corresponded to grasses of the Panicum genus (P. maximum cultivars Tanzânia and Mombaça) and the Brachiaria genus (B. brizantha cultivars Piatã, Marandu, Xaraés, Capiporã and Arapoty; B. decumbens cultivar Basilisk; B. humidicola cultivars Comum and Tupi), evaluated under free growth conditions.. Response variables were leaf appearance and elongation rates, phyllochron, stem elongation rate, final leaf length, number of live leaves per tiller, leaf life span and tiller appearance, death and survival rates. There was difference between cultivars for these variables, indicating a large variability within plants. Considering the morphogenetic and structural variables of leaves and tillers, B. brizantha had a similar pattern of variation to those of P. maximum, and B. Decumbens, similar to those of B. humidicola. Tiller appearance was large at the onset of the experiment and second and third generations comprised the large majority of tiller population for the cultivars Mombaça, Tanzânia, Xaraés and Capiporã. Group analysis based on plant morphogenetic and structural characteristics of leaves and tillers was an interesting way of grouping cultivars, indicating the importance and the potential of using morphogenesis in plant selection and evaluation programmes. RESUMO - O estudo das características morfogênicas permite compreender aspectos relativos à forma e função das plantas forrageiras além de possibilitar identificar plantas com potenciais produtivos e exigências de manejo diferentes. O objetivo deste trabalho foi avaliar, comparativamente, dez plantas forrageiras tropicais por meio de variáveis morfogênicas e estruturais. Os tratamentos corresponderam a cultivares pertencentes ao gênero Panicum (P. maximum cvs. Tanzânia e Mombaça) e ao gênero Brachiaria (B. brizantha cvs. Piatã, Marandu, Xaraés, Capiporã, e Arapoty; B. decumbens cv. Basilisk; B. humidicola cvs. Comum e Tupi), avaliados sob condições de crescimento livre. Foram avaliadas a taxa de aparecimento e alongamento de folhas, filocrono, taxa de alongamento de colmos, comprimento final da lâmina foliar, número de folhas vivas por perfilho, duração de vida das folhas e padrão demográfico de perfilhamento. Houve diferença entre cultivares para as variáveis, indicando grande variabilidade no material estudado. Considerando as características morfogênicas e estruturais de folhas e perfilhos, as B. brizantha apresentaram comportamento parecido com os P. maximum, e B. decumbens mais próximo das B. humidicola. Verificou-se que o aparecimento de perfilhos foi inicialmente elevado e que a segunda e, ou, terceira gerações contribuíram de forma significativa para o número total de perfilhos dos cultivares Mombaça, Tanzânia, Xaraés e Capiporã. A análise de agrupamento baseada nas características morfogênicas e estruturais de folhas e perfilhos foi um método interessante para agrupar cultivares, demonstrando a importância e o potencial de uso da morfogênese em trabalhos de seleção e avaliação de plantas forrageiras.
650 $aForage grasses
650 $aMorphogenesis
650 $aPanicum
650 $aBrachiaria Brizantha
650 $aBrachiaria Decumbens
650 $aBrachiaria Humidicola
650 $aGramínea Forrageira
650 $aMorfogênese
650 $aPanicum Maximum
650 $aPastagem
650 $aPlanta Forrageira
653 $aBraquiária
700 1 $aNASCIMENTO JÚNIOR, D. do
700 1 $aSILVA, S. C. da
700 1 $aEUCLIDES, V. P. B.
700 1 $aMONTAGNER, D. B.
700 1 $aSBRISSIA, A. F.
700 1 $aRODRIGUES. C. S.
700 1 $aSOUSA, B. M. de L.
700 1 $aPENA, K. da S.
700 1 $aVILELA, H. H.
773 $tScientia Agricola$gv. 67, n. 2, p. 136-142, Mar./Abr. 2010.
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