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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
16/06/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
OLINDA, R. G.; MAIA, L. A.; CARGNELUTTI, J. F.; GOIS, R. C. S.; BATISTA, J. S.; DANTAS, A. F. M.; FLORES, E. F.; RIET-CORREA, F. |
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Afiliação: |
ROBERIO G. OLINDA, UFCG; LISANKA A. MAIA, UFCG; JULIANA F. CARGNELUTTI, UFSM; RAYR C. S. GOIS, Universidade Federal Rural do Semi-Árido; JAEL S. BATISTA, Universidade Federal Rural do Semi-Árido; ANTÔNIO F. M. DANTAS, UFCG; EDUARDO F. FLORES, UFSM; FRANKLIN RIET-CORREA, UFCG. |
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Título: |
Swinepox dermatitis in backyard pigs in Northeastern Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 6, p. 468-472, junho. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
This article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvirus is present in backyard pigs in Northeastern Brazil, indicating the need of including SWPV in the differential diagnosis of dermatitis in pigs. MenosThis article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvi... Mostrar Tudo |
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Palavras-Chave: |
Doença viral. |
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Thesagro: |
Dermatite; Suíno; Varíola. |
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Thesaurus Nal: |
Dermatitis; Poxviridae; Swine; Viral diseases of animals and humans. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144459/1/Swinepox-dermatitis.pdf
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Marc: |
LEADER 02469naa a2200301 a 4500
001 2047167
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aOLINDA, R. G.
245 $aSwinepox dermatitis in backyard pigs in Northeastern Brazil.
260 $c2016
520 $aThis article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvirus is present in backyard pigs in Northeastern Brazil, indicating the need of including SWPV in the differential diagnosis of dermatitis in pigs.
650 $aDermatitis
650 $aPoxviridae
650 $aSwine
650 $aViral diseases of animals and humans
650 $aDermatite
650 $aSuíno
650 $aVaríola
653 $aDoença viral
700 1 $aMAIA, L. A.
700 1 $aCARGNELUTTI, J. F.
700 1 $aGOIS, R. C. S.
700 1 $aBATISTA, J. S.
700 1 $aDANTAS, A. F. M.
700 1 $aFLORES, E. F.
700 1 $aRIET-CORREA, F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 6, p. 468-472, junho. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/07/2011 |
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Data da última atualização: |
27/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
VIGNA, B. B. Z.; CANCADO, L. J.; FRANCISCO, P. M.; VALLE, C. B. do; ZUCCHI, M. I.; SOUZA, A. P. |
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Afiliação: |
BIANCA BACCILI ZANOTTO VIGNA, CPPSE; LETICIA JUNGMANN CANCADO, CNPGC; PATRICIA MARA FRANCISCO; CACILDA BORGES DO VALLE, CNPGC; MARIA IMACULADA ZUCCHI; ANETE PEREIRA SOUZA. |
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Título: |
Genetic diversity and population structure of the Brachiaria brizantha germplasm. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Tropical Plant Biology, v. 4, p. 157-169, 2011. |
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DOI: |
10.1007/s12042-011-9078-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Brachiaria brizantha (Hochst. ex A. Rich.) Stapf. (syn. Urochloa brizantha (Hochst. ex A. Rich.) R.D. Webster) is a species used primarily as forage in tropical America and Southeast Asia. B. brizantha has been extensively researched since the 1980s with the initiation of the Tropical Forages Breeding Program conducted by the Brazilian Agricultural Research Corporation (Empresa Brasileira de Pesquisa Agropecuária; EMBRAPA), holding one of the largest germplasm collections in the world. This work has identified 15 new microsatellite markers for this species, which have been used in addition to five previously reported markers, to estimate the genetic similarities among 172 accessions and six cultivars of this species. Similarity index values ranged from 0.40 to 1.00. Two duplications were found in the germplasm. A Bayesian analysis performed using the STRUCTURE 2.3.3 program revealed the presence of three clusters with different allelic pools. This analysis is valuable for the performance of crosses to explore heterosis; however, the mode of reproduction of the accessions and ploidy barriers must be observed for effective exploration. A grouping analysis using the neighbor-joining method was consistent with the STRUCTURE analysis, and a combination approach suggested that this germplasm collection does not exhibit considerable genetic variability despite the presence of three distinct allelic pools. The lack of correlation between the genetic and geographic distances is also discussed. MenosBrachiaria brizantha (Hochst. ex A. Rich.) Stapf. (syn. Urochloa brizantha (Hochst. ex A. Rich.) R.D. Webster) is a species used primarily as forage in tropical America and Southeast Asia. B. brizantha has been extensively researched since the 1980s with the initiation of the Tropical Forages Breeding Program conducted by the Brazilian Agricultural Research Corporation (Empresa Brasileira de Pesquisa Agropecuária; EMBRAPA), holding one of the largest germplasm collections in the world. This work has identified 15 new microsatellite markers for this species, which have been used in addition to five previously reported markers, to estimate the genetic similarities among 172 accessions and six cultivars of this species. Similarity index values ranged from 0.40 to 1.00. Two duplications were found in the germplasm. A Bayesian analysis performed using the STRUCTURE 2.3.3 program revealed the presence of three clusters with different allelic pools. This analysis is valuable for the performance of crosses to explore heterosis; however, the mode of reproduction of the accessions and ploidy barriers must be observed for effective exploration. A grouping analysis using the neighbor-joining method was consistent with the STRUCTURE analysis, and a combination approach suggested that this germplasm collection does not exhibit considerable genetic variability despite the presence of three distinct allelic pools. The lack of correlation between the genetic and geographic distances is also ... Mostrar Tudo |
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Palavras-Chave: |
Forage grass; Molecular markers; Polyploid; Simple sequence repeats. |
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Thesaurus NAL: |
Urochloa brizantha. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02253naa a2200253 a 4500
001 1895273
005 2022-07-27
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s12042-011-9078-1$2DOI
100 1 $aVIGNA, B. B. Z.
245 $aGenetic diversity and population structure of the Brachiaria brizantha germplasm.$h[electronic resource]
260 $c2011
520 $aBrachiaria brizantha (Hochst. ex A. Rich.) Stapf. (syn. Urochloa brizantha (Hochst. ex A. Rich.) R.D. Webster) is a species used primarily as forage in tropical America and Southeast Asia. B. brizantha has been extensively researched since the 1980s with the initiation of the Tropical Forages Breeding Program conducted by the Brazilian Agricultural Research Corporation (Empresa Brasileira de Pesquisa Agropecuária; EMBRAPA), holding one of the largest germplasm collections in the world. This work has identified 15 new microsatellite markers for this species, which have been used in addition to five previously reported markers, to estimate the genetic similarities among 172 accessions and six cultivars of this species. Similarity index values ranged from 0.40 to 1.00. Two duplications were found in the germplasm. A Bayesian analysis performed using the STRUCTURE 2.3.3 program revealed the presence of three clusters with different allelic pools. This analysis is valuable for the performance of crosses to explore heterosis; however, the mode of reproduction of the accessions and ploidy barriers must be observed for effective exploration. A grouping analysis using the neighbor-joining method was consistent with the STRUCTURE analysis, and a combination approach suggested that this germplasm collection does not exhibit considerable genetic variability despite the presence of three distinct allelic pools. The lack of correlation between the genetic and geographic distances is also discussed.
650 $aUrochloa brizantha
653 $aForage grass
653 $aMolecular markers
653 $aPolyploid
653 $aSimple sequence repeats
700 1 $aCANCADO, L. J.
700 1 $aFRANCISCO, P. M.
700 1 $aVALLE, C. B. do
700 1 $aZUCCHI, M. I.
700 1 $aSOUZA, A. P.
773 $tTropical Plant Biology$gv. 4, p. 157-169, 2011.
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