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1. | | RESENDE, R. T.; RESENDE, M. D. V. de; AZEVEDO, C. F.; SILVA, F. F. e; MELO, L. C.; PEREIRA, H. S.; SOUZA, T. L. P. O. de; VALDISSER, P. A. M. R.; BRONDANI, C.; VIANELLO, R. P. Genome-wide association and regional heritability mapping of plant architecture, lodging and productivity in Phaseolus vulgaris. G3: Genes, Genomes, Genetics, v. 8, p. 2841-2854, Aug. 2018. Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Florestas. |
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Registros recuperados : 1 | |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
26/09/2017 |
Data da última atualização: |
14/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P. |
Afiliação: |
WENDELL JACINTO PEREIRA, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 2, p. 150-158, abr./jun. 2017. |
ISSN: |
1518-7853 |
DOI: |
10.1590/1984-70332017v17n2a22 |
Idioma: |
Inglês |
Conteúdo: |
An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris. |
Palavras-Chave: |
Gene normalization; Grain RNA extraction; Molecular breeding. |
Thesagro: |
Feijão; Melhoramento; Phaseolus vulgaris; RNA. |
Thesaurus NAL: |
Beans; Gene expression. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164321/1/CNPAF-2017-cbab.pdf
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Marc: |
LEADER 01868naa a2200289 a 4500 001 2076282 005 2017-12-14 008 2017 bl uuuu u00u1 u #d 022 $a1518-7853 024 7 $a10.1590/1984-70332017v17n2a22$2DOI 100 1 $aPEREIRA, W. J. 245 $aAn improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR.$h[electronic resource] 260 $c2017 520 $aAn RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris. 650 $aBeans 650 $aGene expression 650 $aFeijão 650 $aMelhoramento 650 $aPhaseolus vulgaris 650 $aRNA 653 $aGene normalization 653 $aGrain RNA extraction 653 $aMolecular breeding 700 1 $aBASSINELLO, P. Z. 700 1 $aBRONDANI, C. 700 1 $aVIANELLO, R. P. 773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 17, n. 2, p. 150-158, abr./jun. 2017.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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