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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
01/10/1998 |
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Data da última atualização: |
01/10/1998 |
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Autoria: |
SOARES FILHO, W. dos S.; PELACANI, C. R.; SOUZA, A. da S.; CUNHA SOBRINHO, A. P. da; ARAUJO, E. F. de. |
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Afiliação: |
EMBRAPA-CNPMF. |
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Título: |
Influencia dos tegumentos externo e interno na germinacao de sementes de citros: implicacoes na sobrevivencia de "seedlings" hibridos |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Cruz das Almas: EMBRAPA-CNPMF, 1994 |
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Volume: |
v.2 |
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Páginas: |
p.475-476 |
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Idioma: |
Português |
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Notas: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA. Resumos... Salvador: SBF, 1994. |
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Conteúdo: |
O presente trabalho teve como objetivo verificar a possibilidade de promover a germinacao de sementes de citros sem a necessidade de realizar o cultivo in vitro de seus embrioes de modo a garantir a sobrevivencia de eventuais "seedlings" hibridos, obtidos a partir de cruzamentos controlados. Foi conduzido no Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical - CNPMF, pertencente a Empresa Brasileira de Pesquisa Agropecuaria - EMBRAPA, localizado no municipio de Cruz das Almas, Reconcavo Baiano, durante o periodo de janeiro a maio de 1994. As variedades estudadas compreenderam: tangerina `Clementina' selecao Palazelli (Citrus clementina), tangerina-tangelo `Lee' [Clementina' x tangelo `Orlando' (pomelo `Duncan' C. paradisi x tangerina `Dancy' C. tangerina)], tangerina `Sunki' (C. sunki), limao `Cravo' selecao Santa Barbara (C. limonia) e tangor `Dweet' [laranja `Mediterranea' (C. sinensis) x tangerina `Dancy']. As duas primeiras sao monoembrionicas, a terceira possui baixo grau de poliembrionia e as ultimas apresentam grau moderado de poliembrionia. |
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Palavras-Chave: |
Melhoramento genetico; Tangerina Clementina; Tangerina Sunki; Tangerina-tangelo Lee; Tangor Dweet. |
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Thesagro: |
Hibridação; Limão Cravo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01989naa a2200289 a 4500
001 1645690
005 1998-10-01
008 1994 bl uuuu u00u1 u #d
100 1 $aSOARES FILHO, W. dos S.
245 $aInfluencia dos tegumentos externo e interno na germinacao de sementes de citros$bimplicacoes na sobrevivencia de "seedlings" hibridos
260 $c1994
300 $ap.475-476 v.2
490 $vv.2
500 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA. Resumos... Salvador: SBF, 1994.
520 $aO presente trabalho teve como objetivo verificar a possibilidade de promover a germinacao de sementes de citros sem a necessidade de realizar o cultivo in vitro de seus embrioes de modo a garantir a sobrevivencia de eventuais "seedlings" hibridos, obtidos a partir de cruzamentos controlados. Foi conduzido no Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical - CNPMF, pertencente a Empresa Brasileira de Pesquisa Agropecuaria - EMBRAPA, localizado no municipio de Cruz das Almas, Reconcavo Baiano, durante o periodo de janeiro a maio de 1994. As variedades estudadas compreenderam: tangerina `Clementina' selecao Palazelli (Citrus clementina), tangerina-tangelo `Lee' [Clementina' x tangelo `Orlando' (pomelo `Duncan' C. paradisi x tangerina `Dancy' C. tangerina)], tangerina `Sunki' (C. sunki), limao `Cravo' selecao Santa Barbara (C. limonia) e tangor `Dweet' [laranja `Mediterranea' (C. sinensis) x tangerina `Dancy']. As duas primeiras sao monoembrionicas, a terceira possui baixo grau de poliembrionia e as ultimas apresentam grau moderado de poliembrionia.
650 $aHibridação
650 $aLimão Cravo
653 $aMelhoramento genetico
653 $aTangerina Clementina
653 $aTangerina Sunki
653 $aTangerina-tangelo Lee
653 $aTangor Dweet
700 1 $aPELACANI, C. R.
700 1 $aSOUZA, A. da S.
700 1 $aCUNHA SOBRINHO, A. P. da
700 1 $aARAUJO, E. F. de
773 $tCruz das Almas: EMBRAPA-CNPMF, 1994
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. |
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Afiliação: |
MAREI BORSCH VON HAEHLING, UNESP; GIOVANNA GABRIELLE CRUVINEL, UNICEP; JOÃO HENRIQUE BARBOSA TOSCANO, UNESP; LUCIANA APARECIDA GIRALDELO, UNICEP; ISABELLA BARBOSA DOS SANTOS, UNESP; SERGIO NOVITA ESTEVES, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; WALDOMIRO BARIONI JUNIOR, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 279, 2020, 109053. |
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Páginas: |
11 p. |
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ISSN: |
0304-4017 |
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DOI: |
https://doi.org/10.1016/j.vetpar.2020.109053 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep. MenosGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an... Mostrar Tudo |
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Palavras-Chave: |
Host resistance; Morada Nova sheep; Parasite challenge; SNPs. |
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Thesaurus NAL: |
Genetic markers. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03156naa a2200325 a 4500
001 2121271
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $ahttps://doi.org/10.1016/j.vetpar.2020.109053$2DOI
100 1 $aHAEHLING, M. B. V.
245 $aFour single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep.
650 $aGenetic markers
653 $aHost resistance
653 $aMorada Nova sheep
653 $aParasite challenge
653 $aSNPs
700 1 $aCRUVINEL, G. G.
700 1 $aTOSCANO, J. H. B.
700 1 $aGIRALDELO, L. A.
700 1 $aSANTOS, I. B. dos
700 1 $aESTEVES, S. N.
700 1 $aBENAVIDES, M. V.
700 1 $aBARIONI JUNIOR, W.
700 1 $aNICIURA, S. C. M.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 279, 2020, 109053.
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Embrapa Pecuária Sudeste (CPPSE) |
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