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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ERGUN; SOUZA, F. V. D. |
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Afiliação: |
ERGUN KAYA, Mugla Sitki Kocman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, v.53, Issue 4,p 410?417,| August 2017. |
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ISSN: |
1475-2689 |
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DOI: |
10.1007/s11627-017-9837-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Conservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties. MenosConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stab... Mostrar Tudo |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $a10.1007/s11627-017-9837-2$2DOI
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245 $aComparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers.$h[electronic resource]
260 $c2017
520 $aConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties.
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant$gv.53, Issue 4,p 410?417,| August 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 8 | |
| 1. |  | LEGESE, M. T.; BEKELE, T.; MATTOS, P. P. de; CANETTI, A.; BRAZ, E. M.; TORRES, C. M. M. E. Population management of Juniperus procera tree from Ethiopia. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 154. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 2. | | TORRES, C. M. M. E.; JACOVINE, L. A. G.; TOLEDO, D. P.; RIBEIRO, S. C.; SOARES, C. P. B. Quantificação da biomassa aérea da cultura de Jatropa curcas L., ao terceiro ano, no município de Viçosa - MG. In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. (Embrapa Florestas. Documentos, 178). Seção: Silvicultura e Produção de Biomassa.| Biblioteca(s): Embrapa Florestas. |
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| 3. | | LEGESE, M. T.; KEDIRKAN, N. Y.; TORRES, C. M. M. E.; VILLA, P. M.; TESFAYE, M. A. Woody species diversity, structure, and carbon stock in a disturbed dry Afromontane Forest in Ethiopia. Pesquisa Florestal Brasileira v.45, n., p., 2025 1983-2605, Embrapa Florestas, 2025 Disponível em: https://doi.org/https://doi.org/10.4336/2025.pfb.45e202302284. Acesso em:| Biblioteca(s): Embrapa Florestas. |
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| 4. | | OLIVEIRA, A. C.; PEREIRA, B. L. C.; CARNEIRO, A. de C. O.; CARDOSO JÚNIOR, J. F.; TORRES, C. M. M. E.; REIS, B. E. Avaliação de diferentes marcas de carvão vegetal comercializados na cidade de Viçosa, MG. In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. (Embrapa Florestas. Documentos, 178). Seção: Matéria Prima e Processos de Conversão Energética.| Biblioteca(s): Embrapa Florestas. |
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| 5. | | TORRES, C. M. M. E.; JACOVINE, L. A. G.; OLIVEIRA NETO, S. N. de; BRIANEZI, D.; ALVES, E. B. B. M. Sistemas agroflorestais no Brasil: uma abordagem sobre a estocagem de carbono. Pesquisa Florestal Brasileira, Colombo, v. 34, n. 79, p. 217-224, jul./set. 2014. Artigo de revisão.| Biblioteca(s): Embrapa Florestas. |
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| 6. | | ANDRADE, C. G. C.; SILVA, M. L. da; TORRES, C. M. M. E.; RUSCHEL, A. R.; SILVA, L. F. da; ANDRADE, D. F. C. de; REIS, L. P. Crescimento diamétrico e tempo de passagem de Minquartia guianensis após manejo na Floresta Nacional do Tapajós. Pesquisa Florestal Brasileira, Colombo, v. 37, n. 91, p. 299-309, jul./set. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Florestas. |
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| 7. | | TESHOME, M.; BRAZ, E. M.; TORRES, C. M. M. E.; RAPTIS, D. I.; MATTOS, P. P. de; TEMESGEN, H.; RUBIO-CAMACHO, E. A.; SILESHI, G. W. Mixed-effects height prediction model for Juniperus procera trees from a Dry Afromontane Forest in Ethiopia. Forests, v. 15, n. 3, 443, p. 1-19, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 8. | | TESHOME, M.; TORRES, C. M. M. E.; SILESHI, G. W.; MATTOS, P. P. de; BRAZ, E. M.; TEMESGEN, H.; ROCHA, S. J. S. S. da; ALEBACHEW, M. Mixed-Species allometric equations to quantify stem volume and tree biomass in Dry Afromontane Forest of Ethiopia. Open Journal of Forestry, v. 12, p. 263-296, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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