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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
14/03/2011 |
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Data da última atualização: |
14/03/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ALMEIDA, J. D. de; BARROS, L. M. G.; SANTOS, D. B. M.; COTTA, M. G.; BARBOSA, E. A.; CAÇÃO, S. B.; EIRA, M. T. S. da; ALVES, G. S. C.; VINECKY, F.; PEREIRA, L. F. P.; SILVA, F. R. da; ANDRADE, A. C.; MARRACCINI, P.; CARNEIRO, M. |
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Afiliação: |
JULIANA DANTAS DE ALMEIDA, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; ALAN CARVALHO ANDRADE, CENARGEN; CIRAD UMR-DAP; MAURO CARNEIRO, CENARGEN. |
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Título: |
Prospection of tissue specific promoters in coffee. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil. |
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Idioma: |
Inglês |
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Conteúdo: |
The majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. MenosThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its resp... Mostrar Tudo |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02510nam a2200277 a 4500
001 1880640
005 2011-03-14
008 2008 bl uuuu u00u1 u #d
100 1 $aALMEIDA, J. D. de
245 $aProspection of tissue specific promoters in coffee.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil.$c2008
520 $aThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented.
653 $aCoffee
700 1 $aBARROS, L. M. G.
700 1 $aSANTOS, D. B. M.
700 1 $aCOTTA, M. G.
700 1 $aBARBOSA, E. A.
700 1 $aCAÇÃO, S. B.
700 1 $aEIRA, M. T. S. da
700 1 $aALVES, G. S. C.
700 1 $aVINECKY, F.
700 1 $aPEREIRA, L. F. P.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
700 1 $aMARRACCINI, P.
700 1 $aCARNEIRO, M.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 23 | |
| 7. |  | ROSA, L. S.; WENDLING, I.; SOUZA JUNIOR, L. Brotações epicórmicas no resgate vegetativo de árvores selecionadas de Ilex paraguariensis St. Hil. In: CONGRESSO SUL-AMERICANO DA ERVA-MATE, 3., 2003, Chapecó. Anais. [Chapecó]: EPAGRI, 2003. s.3-2. 1 CD-ROM. Secão: Conservação, Melhoramento e Multiplicação. Feira do Agronegócio da Erva-mate, 1., 2003, Chapecó. Integrar para promover o agronegócio da erva-mate.| Biblioteca(s): Embrapa Florestas. |
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| 9. | | SOUZA JÚNIOR, L.; WENDLING, I; SANTOS, D. C. dos. Fitorreguladores de crescimento no alongamento in vitro de Pinus greggii. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 2., 2003, Colombo. Anais. Colombo: Embrapa Florestas, 2003. 027A. 1 Cd-Rom. (Embrapa Florestas. Documentos, 86). Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 11. | | ARRUDA, P. M.; SOUSA, L. P. de; WENDLING, I.; ROSA, L. S. da; SOUZA JUNIOR, L. Avaliação do grau de germinação de sementes e do crescimento de mudas de Mimosa scabrella Benthan em tubetes sob diferentes substratos. In: CONGRESSO FLORESTAL BRASILEIRO, 8., 2003, São Paulo. Benefícios, Produtos e Serviços da Floresta: Oportunidades e Desafios do Século XXI. São Paulo: Sociedade Brasileira de Silvicultura: Sociedade Brasileira de Engenheiros Florestais, 2003. CD-ROM. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 12. | | CUNHA, A. C. M. C. M. da; WENDLING, I.; SOUZA JÚNIOR, L.; ROSA, L. S. da. Efeito de diferentes substratos na produção de mudas Myrsine ferruginea Sprenge. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 2., 2003, Colombo. Anais. Colombo: Embrapa Florestas, 2003. 026A. 1 Cd-Rom. (Embrapa Florestas. Documentos, 86). Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 16. | | CUNHA, A. C. M. C. M. da; WENDLING, I.; SOUZA JUNIOR, L. Influência da presença ou ausência de folhas no enraizamento de miniestacas de Erythrina falcata Bentham obtidas de sistema hidropônico. In: CONGRESSO NACIONAL DE BOTÂNICA, 55.; REUNIÃO REGIONAL DE BOTÂNICOS DE MG, BA E ES, 26., 2004, Viçosa. Conservação, bioprospecção e biotecnologia:[resumos]. Viçosa: Sociedade Botânica do Brasil: Universidade Federal Viçosa, 2004. CD ROM Resumo.| Biblioteca(s): Embrapa Florestas. |
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| 20. | | NAZÁRIO, P.; SOUSA, L. P. de; WENDLING, I.; CUNHA, A. C. M. C. M. da; SOUZA JÚNIOR, L. Estaquia de Luehea divaricata (Tiliaceae) sob diferentes concentrações de regulador de crescimento para enraizamento AIB (Ácido Indol-Butírico). In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 2., 2003, Colombo. Anais. Colombo: Embrapa Florestas, 2003. 022R. 1 Cd-Rom. (Embrapa Florestas. Documentos, 86). Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 23 | |
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