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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/07/2024 |
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Data da última atualização: |
09/07/2024 |
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Autoria: |
BAGUISI, A.; BEHBOODI, E.; MELICAN, D. T.; POLLOCK, J. S.; DESTREMPES, M. M.; CAMMUSO, C.; WILLIAMS, J. L.; NIMS, S. D.; PORTER, C. A.; MIDURA, P.; PALACIOS, M. J.; AYRES, S. L.; DENNISTON, R. S.; HAYES, M. L.; ZIOMEK, C. A.; MEADE, H. M.; GODKE, R. A.; GAVIN, W. G.; OVERSTRÖM, E. W.; ECHELARD, Y. |
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Título: |
Production of goats by somatic cell nuclear transfer. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Nature Biotechnology, v. 17, n. 5, p. 456-461, May, 1999. |
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DOI: |
https://doi.org/10.1038/8632 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In this study, we demonstrate the production of transgenic goats by nuclear transfer of fetal somatic cells. Donor karyoplasts were obtained from a primary fetal somatic cell line derived from a 40-day transgenic female fetus produced by artificial insemination of a nontransgenic adult female with semen from a transgenic male. Live offspring were produced with two nuclear transfer procedures. In one protocol, oocytes at the arrested metaphase II stage were enucleated, electrofused with donor somatic cells, and simultaneously activated. In the second protocol, activated in vivo oocytes were enucleated at the telophase II stage, electrofused with donor somatic cells, and simultaneously activated a second time to induce genome reactivation. Three healthy identical female offspring were born. Genotypic analyses confirmed that all cloned offspring were derived from the donor cell line. Analysis of the milk of one of the transgenic cloned animals showed high-level production of human antithrombin III, similar to the parental transgenic line. |
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Palavras-Chave: |
Embryonic and fetal development; Genetically modified; Nuclear transfer techniques. |
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Thesaurus Nal: |
Genetics; Goats; In situ hybridization; Polymerase chain reaction; Pregnancy; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02350naa a2200469 a 4500
001 2165588
005 2024-07-09
008 1999 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/8632$2DOI
100 1 $aBAGUISI, A.
245 $aProduction of goats by somatic cell nuclear transfer.$h[electronic resource]
260 $c1999
520 $aAbstract: In this study, we demonstrate the production of transgenic goats by nuclear transfer of fetal somatic cells. Donor karyoplasts were obtained from a primary fetal somatic cell line derived from a 40-day transgenic female fetus produced by artificial insemination of a nontransgenic adult female with semen from a transgenic male. Live offspring were produced with two nuclear transfer procedures. In one protocol, oocytes at the arrested metaphase II stage were enucleated, electrofused with donor somatic cells, and simultaneously activated. In the second protocol, activated in vivo oocytes were enucleated at the telophase II stage, electrofused with donor somatic cells, and simultaneously activated a second time to induce genome reactivation. Three healthy identical female offspring were born. Genotypic analyses confirmed that all cloned offspring were derived from the donor cell line. Analysis of the milk of one of the transgenic cloned animals showed high-level production of human antithrombin III, similar to the parental transgenic line.
650 $aGenetics
650 $aGoats
650 $aIn situ hybridization
650 $aPolymerase chain reaction
650 $aPregnancy
650 $aReproduction
653 $aEmbryonic and fetal development
653 $aGenetically modified
653 $aNuclear transfer techniques
700 1 $aBEHBOODI, E.
700 1 $aMELICAN, D. T.
700 1 $aPOLLOCK, J. S.
700 1 $aDESTREMPES, M. M.
700 1 $aCAMMUSO, C.
700 1 $aWILLIAMS, J. L.
700 1 $aNIMS, S. D.
700 1 $aPORTER, C. A.
700 1 $aMIDURA, P.
700 1 $aPALACIOS, M. J.
700 1 $aAYRES, S. L.
700 1 $aDENNISTON, R. S.
700 1 $aHAYES, M. L.
700 1 $aZIOMEK, C. A.
700 1 $aMEADE, H. M.
700 1 $aGODKE, R. A.
700 1 $aGAVIN, W. G.
700 1 $aOVERSTRÖM, E. W.
700 1 $aECHELARD, Y.
773 $tNature Biotechnology$gv. 17, n. 5, p. 456-461, May, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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