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| Registros recuperados : 35 | |
| Registros recuperados : 35 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
22/09/2017 |
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Data da última atualização: |
30/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SOUZA, R. A. V. de; ALVES, M. de C.; CARNEIRO, N. P.; BARROS, B. de A.; BOREM, A.; CARNEIRO, A. A. |
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Afiliação: |
Rafaeli Aparecida Vieira de Souza, Universidade Federal de Viçosa; MEIRE DE CASSIA ALVES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; Aluízio Borém, Universidade Federal de Viçosa; ANDREA ALMEIDA CARNEIRO, CNPMS. |
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Título: |
Agrobacterium-mediated genetic transformation of a tropical elite maize line. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, p. 133-140, 2017. |
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DOI: |
10.1590/1984-70332017v17n2a20 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens. |
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Palavras-Chave: |
Concentração de sal; Transformação genética. |
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Thesagro: |
Bactéria; Embriogênese; Genética. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164228/1/Agrobacterium-mediated-1.pdf
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Marc: |
LEADER 01738naa a2200253 a 4500
001 2076138
005 2018-01-30
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1590/1984-70332017v17n2a20$2DOI
100 1 $aSOUZA, R. A. V. de
245 $aAgrobacterium-mediated genetic transformation of a tropical elite maize line.$h[electronic resource]
260 $c2017
520 $aABSTRACT: The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% ? half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embryos were transformed via A. tumefaciens harboring the binary vector pTF102 containing the genes uidA and BAR under control of the CaMV35S promoter. The efficiency of the transgenic events produced was 3% for full strength and 1.1% for half strength N6 salts. Although under a lower concentration of salts, GUS expression was stronger; under this concentration, regeneration was less efficient. Thus, the results showed that the presence of 100% N6 salts in the infection and cocultivation media favored genetic transformation of the L3 maize inbred line mediated by A. tumefaciens.
650 $aBactéria
650 $aEmbriogênese
650 $aGenética
653 $aConcentração de sal
653 $aTransformação genética
700 1 $aALVES, M. de C.
700 1 $aCARNEIRO, N. P.
700 1 $aBARROS, B. de A.
700 1 $aBOREM, A.
700 1 $aCARNEIRO, A. A.
773 $tCrop Breeding and Applied Biotechnology, Viçosa, MG$gv. 17, p. 133-140, 2017.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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