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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
01/12/2015 |
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Data da última atualização: |
24/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LUZ, G. A.; GOMES, S. O.; ARAUJO NETO, R. B. de; NASCIMENTO, M. S. C. B.; LIMA, P. S. da C. |
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Afiliação: |
G.A. Luz, Laboratório de Biologia Molecular, Centro de Ciências Agrárias, Universidade Federal do Piauí, Teresina, PI.; S.O. Gomes, Laboratório de Biologia Molecular, Centro de Ciências Agrárias, Universidade Federal do Piauí, Teresina, PI.; RAIMUNDO BEZERRA DE ARAUJO NETO, CPAMN; M.S.C.B. Nascimento, APOSENTADA; PAULO SARMANHO DA COSTA LIMA, CPAMN. |
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Título: |
Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 4, p. 15242-15248, nov. 2015. |
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ISSN: |
1676-5680 |
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DOI: |
http://dx.doi.org/10.4238/2015.November.25.12 |
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Idioma: |
Inglês |
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Conteúdo: |
Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of considerable variability within the collection. Potential progenitors were selected that would offer good possibilities of obtaining unusual and favorable combinations of genes in a plant breeding program. MenosCratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of conside... Mostrar Tudo |
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Palavras-Chave: |
Caracterização molecular; Diversidade genética; Forage crop; Genetic diversity; ISSR markers; Molecular characterization. |
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Thesagro: |
Marcador molecular; Planta forrageira. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134533/1/ArtigoSarmanhoGMR6985cam.pdf
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Marc: |
LEADER 02619naa a2200289 a 4500
001 2030223
005 2022-05-24
008 2015 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $ahttp://dx.doi.org/10.4238/2015.November.25.12$2DOI
100 1 $aLUZ, G. A.
245 $aMolecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers.
260 $c2015
520 $aCratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of considerable variability within the collection. Potential progenitors were selected that would offer good possibilities of obtaining unusual and favorable combinations of genes in a plant breeding program.
650 $aMarcador molecular
650 $aPlanta forrageira
653 $aCaracterização molecular
653 $aDiversidade genética
653 $aForage crop
653 $aGenetic diversity
653 $aISSR markers
653 $aMolecular characterization
700 1 $aGOMES, S. O.
700 1 $aARAUJO NETO, R. B. de
700 1 $aNASCIMENTO, M. S. C. B.
700 1 $aLIMA, P. S. da C.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 14, n. 4, p. 15242-15248, nov. 2015.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/11/2023 |
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Data da última atualização: |
15/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VILAR, C. S. M. M.; MALHEIROS, J. M.; SILVA, P. F. da; MARTINS, E. H.; CORREIA, L. E. C. S.; OLIVEIRA, M. H. V. de; COLNAGO, L. A.; SILVA, J. A. II. V.; MERCADANTE, M. E. Z. |
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Afiliação: |
Beef Cattle Research Center, Animal Science Institute (IZ); Beef Cattle Research Center, Animal Science Institute (IZ); Institute of Chemistry, University of Sao Paulo (USP); School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP); School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP); School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP); LUIZ ALBERTO COLNAGO, CNPDIA; School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP). |
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Título: |
Muscle growth affects the metabolome of the pectoralis major muscle in redwinged tinamou (Rhynchotus rufescens). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Poultry Science, v. 102, n. 12, 2023. |
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Páginas: |
15 p. |
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DOI: |
https://doi.org/10.1016/j.psj.2023.103104 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT The aim of the present study was to identify and quantify the metabolites (metabolome analysis) of the pectoralis major muscle in male redwinged tinamou (Rhynchotus rufescens) selected for growth traits. A selection index was developed for females [body weight (BW), chest circumference (CC), and thigh circumference (TC)] and males [BW, CC, TC, semen volume, and sperm concentration] in order to divide the animals into 2 experimental groups: selection group with a higher index (TinamouS) and commercial group with a lower index (TinamouC). Twenty male offspring of the 2 groups (TinamouS, n = 10; TinamouC, n = 10) were confined for 350 d. The birds were slaughtered and pectoralis major muscle samples were collected, subjected to polar and apolar metabolites extractions and analyzed by proton nuclear magnetic resonance (1 H NMR) spectroscopy. Analysis of the polar metabolomic profile identified 65 metabolites; 29 of them were differentially expressed between the experimental groups (P < 0.05). The TinamouS groups exhibited significantly higher concentrations (P < 0.05) of 25 metabolites, including anserine, aspartate, betaine, carnosine, creatine, glutamate, threonine, 3-methylhistidine, NAD+, pyruvate, and taurine. Significantly higher concentrations of cysteine, beta-alanine, lactose, and choline were observed in the TinamouC group (P < 0.05). The metabolites identified in the muscle provided information about the main metabolic pathways (higher impact value and P < 0.05), for example, phenylalanine, tyrosine and tryptophan biosynthesis; alanine, aspartate and glutamate metabolism; D-glutamine and D-glutamate metabolism; b-alanine metabolism; glycine, serine and threonine metabolism; taurine and hypotaurine metabolism; histidine metabolism; phenylalanine metabolism. The NMR spectra of apolar fraction showed 8 classes of chemical compounds. The metabolome analysis shows that the selection index resulted in the upregulation of polyunsaturated fatty acids, unsaturated fatty acids, phosphocholines, phosphoethanolamines, triacylglycerols, and glycerophospholipids. The present study suggests that, despite few generations, the selection based on muscle growth traits promoted changes in metabolite concentrations in red-winged tinamou.based on muscle growth traits promoted changes in metabolite concentrations in red-winged tinamou. MenosABSTRACT The aim of the present study was to identify and quantify the metabolites (metabolome analysis) of the pectoralis major muscle in male redwinged tinamou (Rhynchotus rufescens) selected for growth traits. A selection index was developed for females [body weight (BW), chest circumference (CC), and thigh circumference (TC)] and males [BW, CC, TC, semen volume, and sperm concentration] in order to divide the animals into 2 experimental groups: selection group with a higher index (TinamouS) and commercial group with a lower index (TinamouC). Twenty male offspring of the 2 groups (TinamouS, n = 10; TinamouC, n = 10) were confined for 350 d. The birds were slaughtered and pectoralis major muscle samples were collected, subjected to polar and apolar metabolites extractions and analyzed by proton nuclear magnetic resonance (1 H NMR) spectroscopy. Analysis of the polar metabolomic profile identified 65 metabolites; 29 of them were differentially expressed between the experimental groups (P < 0.05). The TinamouS groups exhibited significantly higher concentrations (P < 0.05) of 25 metabolites, including anserine, aspartate, betaine, carnosine, creatine, glutamate, threonine, 3-methylhistidine, NAD+, pyruvate, and taurine. Significantly higher concentrations of cysteine, beta-alanine, lactose, and choline were observed in the TinamouC group (P < 0.05). The metabolites identified in the muscle provided information about the main metabolic pathways (higher impact value and P < 0.... Mostrar Tudo |
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Palavras-Chave: |
Muscle growth; NMR. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158669/1/P-Muscle-growth-affects-the-metabolome-of-the-pectoralis-major-muscle-in-redwinged-tinamou-Rhynchotus-rufescens.pdf
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Marc: |
LEADER 03189naa a2200265 a 4500
001 2158669
005 2024-01-15
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.psj.2023.103104$2DOI
100 1 $aVILAR, C. S. M. M.
245 $aMuscle growth affects the metabolome of the pectoralis major muscle in redwinged tinamou (Rhynchotus rufescens).$h[electronic resource]
260 $c2023
300 $a15 p.
520 $aABSTRACT The aim of the present study was to identify and quantify the metabolites (metabolome analysis) of the pectoralis major muscle in male redwinged tinamou (Rhynchotus rufescens) selected for growth traits. A selection index was developed for females [body weight (BW), chest circumference (CC), and thigh circumference (TC)] and males [BW, CC, TC, semen volume, and sperm concentration] in order to divide the animals into 2 experimental groups: selection group with a higher index (TinamouS) and commercial group with a lower index (TinamouC). Twenty male offspring of the 2 groups (TinamouS, n = 10; TinamouC, n = 10) were confined for 350 d. The birds were slaughtered and pectoralis major muscle samples were collected, subjected to polar and apolar metabolites extractions and analyzed by proton nuclear magnetic resonance (1 H NMR) spectroscopy. Analysis of the polar metabolomic profile identified 65 metabolites; 29 of them were differentially expressed between the experimental groups (P < 0.05). The TinamouS groups exhibited significantly higher concentrations (P < 0.05) of 25 metabolites, including anserine, aspartate, betaine, carnosine, creatine, glutamate, threonine, 3-methylhistidine, NAD+, pyruvate, and taurine. Significantly higher concentrations of cysteine, beta-alanine, lactose, and choline were observed in the TinamouC group (P < 0.05). The metabolites identified in the muscle provided information about the main metabolic pathways (higher impact value and P < 0.05), for example, phenylalanine, tyrosine and tryptophan biosynthesis; alanine, aspartate and glutamate metabolism; D-glutamine and D-glutamate metabolism; b-alanine metabolism; glycine, serine and threonine metabolism; taurine and hypotaurine metabolism; histidine metabolism; phenylalanine metabolism. The NMR spectra of apolar fraction showed 8 classes of chemical compounds. The metabolome analysis shows that the selection index resulted in the upregulation of polyunsaturated fatty acids, unsaturated fatty acids, phosphocholines, phosphoethanolamines, triacylglycerols, and glycerophospholipids. The present study suggests that, despite few generations, the selection based on muscle growth traits promoted changes in metabolite concentrations in red-winged tinamou.based on muscle growth traits promoted changes in metabolite concentrations in red-winged tinamou.
653 $aMuscle growth
653 $aNMR
700 1 $aMALHEIROS, J. M.
700 1 $aSILVA, P. F. da
700 1 $aMARTINS, E. H.
700 1 $aCORREIA, L. E. C. S.
700 1 $aOLIVEIRA, M. H. V. de
700 1 $aCOLNAGO, L. A.
700 1 $aSILVA, J. A. II. V.
700 1 $aMERCADANTE, M. E. Z.
773 $tPoultry Science$gv. 102, n. 12, 2023.
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Embrapa Instrumentação (CNPDIA) |
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