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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/09/2018 |
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Data da última atualização: |
31/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; ROGERIO, L. A.; RIBEIRO-ALVES, M.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. |
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Afiliação: |
GABRIELLA D. ARENA, Instituto Agronômico de Campinas; PEDRO L. RAMOS-GONZÁLEZ, Universidade Estadual de Campinas; LUANA A. ROGERIO, Instituto Agronômico de Campinas; MARCELO RIBEIRO-ALVES, Fundação Oswaldo Cruz; CLARE L. CASTEEL, University of California; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Instituto Agronômico de Campinas. |
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Título: |
Making a better home: modulation of plant defensive response by brevipalpus mites. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v.9, August, 2018. |
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ISSN: |
1664-462X |
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DOI: |
10.3389/fpls.2018.01147 |
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Idioma: |
Inglês |
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Conteúdo: |
False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growthdefense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the noninfested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JAmediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway. MenosFalse-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growthdefense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicyl... Mostrar Tudo |
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Thesaurus Nal: |
Herbivores; Jasmonic acid; Plant hormones; Salicylic acid. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182697/1/fpls-09-01147.pdf
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Marc: |
LEADER 03239naa a2200265 a 4500
001 2095414
005 2018-10-31
008 2018 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2018.01147$2DOI
100 1 $aARENA, G. D.
245 $aMaking a better home$bmodulation of plant defensive response by brevipalpus mites.$h[electronic resource]
260 $c2018
520 $aFalse-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growthdefense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the noninfested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA- or JAmediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway.
650 $aHerbivores
650 $aJasmonic acid
650 $aPlant hormones
650 $aSalicylic acid
700 1 $aRAMOS-GONZÁLEZ, P. L.
700 1 $aROGERIO, L. A.
700 1 $aRIBEIRO-ALVES, M.
700 1 $aCASTEEL, C. L.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
773 $tFrontiers in Plant Science$gv.9, August, 2018.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Classificação |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/08/2000 |
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Data da última atualização: |
09/06/2018 |
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Autoria: |
LANZA, M. A.; GUIMARAES, C. T.; SCHUSTER, I. |
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Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Aplicação de marcadores moleculares no melhoramento genético. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Informe Agropecuário, Belo Horizonte, v. 21, n. 204, p. 97-108, maio/jun. 2000. |
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Idioma: |
Português |
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Conteúdo: |
O melhoramento genetico tem sido um dos grandes responsaveis pelos avancos na agricultura, com o desenvolvimento de cultivares superiores, quer pela maior produtividade, quer pela melhor adaptacao aos ambientes adversos. O sucesso de um programa de melhoramento genetico depende fundamentalmente de algumas etapas como a escolha de genitores que produzam individuos com a melhor combinacao de alelos favoraveis e a selecao de genotipos superiores em populacoes segregantes. Uma potente ferramenta para auxiliar o melhoramento sao os marcadores moleculares, que fornecem um numero ilimitado de polimorfismos com base do DNA e sao independentes dos efeitos ambientais e do estadio fisiologico da planta, permitindo a identificacao precoce e precisa de individuos com uma melhor combinacao de elelos favoraveis. Os avancos nas tecnicas moleculares, na bioinformatica e na genetica quantitativa tem contribuido de forma sinergistica para o atual nivel de conhecimentos sobre a estrutura genetica de varias especies cultivadas e silvestres. Assim, varias sao as aplicacoes dos marcadores moleculares, tanto para auxiliar programas de melhoramento, quanto em estudos de variabilidade genetica, identificacao de cultivares, protecao dos direitos do melhorista, avaliacao da pureza genetica de sementes, mapeamento genetico e ampliacao dos conhecimentos na organizacao dos genomas. |
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Palavras-Chave: |
Alelos; Genetic variability; Molecular marker; Variabilidade genetica. |
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Thesagro: |
Marcador Molecular; Polimorfismo. |
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Thesaurus NAL: |
genome. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45526/1/Aplicacao-marcadores.pdf
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Marc: |
LEADER 02043naa a2200229 a 4500
001 1484109
005 2018-06-09
008 2000 bl uuuu u00u1 u #d
100 1 $aLANZA, M. A.
245 $aAplicação de marcadores moleculares no melhoramento genético.$h[electronic resource]
260 $c2000
520 $aO melhoramento genetico tem sido um dos grandes responsaveis pelos avancos na agricultura, com o desenvolvimento de cultivares superiores, quer pela maior produtividade, quer pela melhor adaptacao aos ambientes adversos. O sucesso de um programa de melhoramento genetico depende fundamentalmente de algumas etapas como a escolha de genitores que produzam individuos com a melhor combinacao de alelos favoraveis e a selecao de genotipos superiores em populacoes segregantes. Uma potente ferramenta para auxiliar o melhoramento sao os marcadores moleculares, que fornecem um numero ilimitado de polimorfismos com base do DNA e sao independentes dos efeitos ambientais e do estadio fisiologico da planta, permitindo a identificacao precoce e precisa de individuos com uma melhor combinacao de elelos favoraveis. Os avancos nas tecnicas moleculares, na bioinformatica e na genetica quantitativa tem contribuido de forma sinergistica para o atual nivel de conhecimentos sobre a estrutura genetica de varias especies cultivadas e silvestres. Assim, varias sao as aplicacoes dos marcadores moleculares, tanto para auxiliar programas de melhoramento, quanto em estudos de variabilidade genetica, identificacao de cultivares, protecao dos direitos do melhorista, avaliacao da pureza genetica de sementes, mapeamento genetico e ampliacao dos conhecimentos na organizacao dos genomas.
650 $agenome
650 $aMarcador Molecular
650 $aPolimorfismo
653 $aAlelos
653 $aGenetic variability
653 $aMolecular marker
653 $aVariabilidade genetica
700 1 $aGUIMARAES, C. T.
700 1 $aSCHUSTER, I.
773 $tInforme Agropecuário, Belo Horizonte$gv. 21, n. 204, p. 97-108, maio/jun. 2000.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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