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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
05/11/2001 |
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Data da última atualização: |
20/02/2009 |
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Autoria: |
MADRUGA, C. R.; MARQUES, A. P. C.; ARAUJO, F. R.; MIGUITA, M.; CARVALHO, C. M. E.; ARAUJO, F. S.; UMAKI, A. C. S.; CROCCI, A. J.; QUEIROZ, R. A. |
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Afiliação: |
Embrapa Gado de Corte (Campo Grande, MS). |
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Título: |
Evaluation of an ELISA for detection of antibodies to Babesia bigemin in cattle and it's application in an epidemiological survey in Brazil. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Pesquisa Veterinaria Brasileira, Rio de Janeiro, v.21, n.2, p.72-76, abr. / jun. 2001. |
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Idioma: |
Inglês |
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Notas: |
CNPGC. |
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Conteúdo: |
An indirect enzyme-linked immunosorbent assay (ELISA) using a crude antigen was evaluated of its performance to detect Babesia antibodies. The sensitivity and specificity were 98,0% and 99,0%, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 10 Babesia bovis organisms. With regard to the comparison of ELISA and indirect fluorescent antibody test. (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves sera. There was a good agreement between results shown by ELISA and IFAT with sera from an enzootically stable area (K=0,61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (K=0,33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalence ranged from 87,7 to 98,9%. Therefore, this ELISA with high sensitivity, specificity and performance similar to IFAT can be employed in serological diagnosis of B. bigemina. |
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Palavras-Chave: |
Brasil; IFAT; Immunofluorescense; Mato Grosso do Sul. |
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Thesagro: |
Babesia Bigemina; Bovino; Elisa; Epidemiologia; Imunofluorescência; Imunologia. |
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Thesaurus Nal: |
Brazil; cattle; epidemiology; immunology; Pantanal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02434naa a2200409 a 4500
001 1324252
005 2009-02-20
008 2001 bl uuuu u00u1 u #d
100 1 $aMADRUGA, C. R.
245 $aEvaluation of an ELISA for detection of antibodies to Babesia bigemin in cattle and it's application in an epidemiological survey in Brazil.
260 $c2001
500 $aCNPGC.
520 $aAn indirect enzyme-linked immunosorbent assay (ELISA) using a crude antigen was evaluated of its performance to detect Babesia antibodies. The sensitivity and specificity were 98,0% and 99,0%, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 10 Babesia bovis organisms. With regard to the comparison of ELISA and indirect fluorescent antibody test. (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves sera. There was a good agreement between results shown by ELISA and IFAT with sera from an enzootically stable area (K=0,61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (K=0,33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalence ranged from 87,7 to 98,9%. Therefore, this ELISA with high sensitivity, specificity and performance similar to IFAT can be employed in serological diagnosis of B. bigemina.
650 $aBrazil
650 $acattle
650 $aepidemiology
650 $aimmunology
650 $aPantanal
650 $aBabesia Bigemina
650 $aBovino
650 $aElisa
650 $aEpidemiologia
650 $aImunofluorescência
650 $aImunologia
653 $aBrasil
653 $aIFAT
653 $aImmunofluorescense
653 $aMato Grosso do Sul
700 1 $aMARQUES, A. P. C.
700 1 $aARAUJO, F. R.
700 1 $aMIGUITA, M.
700 1 $aCARVALHO, C. M. E.
700 1 $aARAUJO, F. S.
700 1 $aUMAKI, A. C. S.
700 1 $aCROCCI, A. J.
700 1 $aQUEIROZ, R. A.
773 $tPesquisa Veterinaria Brasileira, Rio de Janeiro$gv.21, n.2, p.72-76, abr. / jun. 2001.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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