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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
30/06/2010 |
Data da última atualização: |
18/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. |
Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP-JABOTICABAL; JORG PATRICK BURGSTALLER, UNIVERSTIY OF VETERINARY MEDICINE - VIENNA; FELIPE PERECIN, UNESP - JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP - JABOTICABAL; MARCOS ROBERTO CHIARATTI, USP - PIRASSUNUNGA; SIMONE CRISTINA MÉO, CPPSE; MATHIAS MULLER, UNIVERSITY OF VETERINARY MEDICINE - VIENNA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL - QUEBEC; FLÁVIO VIEIRA MEIRELLES, USP - PIRASSUNUNGA; RALF STEINBORN, UNIVERSITY OF VETERINARY MEDICINE - VIENNA. |
Título: |
Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010. |
DOI: |
https://doi.org/10.1095/biolreprod.109.080564 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. |
Palavras-Chave: |
Bovine; Developmental biology; Embryo; Gamete biology; Ooplasmic. |
Thesagro: |
DNA. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28805/1/PROCISCMN2010.00002.pdf
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Marc: |
LEADER 02370naa a2200313 a 4500 001 1856388 005 2022-11-18 008 2010 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1095/biolreprod.109.080564$2DOI 100 1 $aFERREIRA, C. R. 245 $aPronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.$h[electronic resource] 260 $c2010 520 $aOoplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. 650 $aDNA 653 $aBovine 653 $aDevelopmental biology 653 $aEmbryo 653 $aGamete biology 653 $aOoplasmic 700 1 $aBURGSTALLER, J. P. 700 1 $aPERECIN, F. 700 1 $aGARCIA, J. M. 700 1 $aCHIARATTI, M. R. 700 1 $aMÉO, S. C. 700 1 $aMULLER, M. 700 1 $aSMITH, L. C. 700 1 $aMEIRELLES, F. V. 700 1 $aSTEINBORN, R. 773 $tBiology of Reproduction$gv. 82, n. 03, p. 563-571, mar. 2010.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
26/12/2022 |
Data da última atualização: |
26/12/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
FARIAS, S. P.; ALMEIDA, A. V. D. L.; SANTOS, S. L.; MOURA FILHO, M.; GOMES, T. C. de A.; ROCHA, F. S.; MUNIZ, M. F. S. |
Afiliação: |
S. P. FARIAS, UFAL; A. V. D. L. ALMEIDA, UFAL; S. L. SANTOS, UFAL; G. MOURA FILHO, UFAL; TAMARA CLAUDIA DE ARAUJO GOMES, CPATC; F. S. ROCHA; M. F. S. MUNIZ, UFAL. |
Título: |
Management of dry rot disease of uam with cassava wastewater. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Nematrópica, v. 52, n. 1, 2022. |
Idioma: |
Inglês |
Conteúdo: |
Dry rot disease caused by the plant-parasitic nematodes Scutellonema bradys, Pratylenchus coffeae, and P. brachyurus limits yam (Dioscorea spp.) yield in Brazil. As a result, the development of strategies to reduce agricultural losses caused by these plant-parasitic nematodes is required. The objective of this study was to evaluate the effect of cassava wastewater (manipueira) on the management of dry rot under two growing conditions (without or with supplemental irrigation) and on soil chemical characteristics. The following treatments were considered: manipueira concentrations diluted in water (10, 25, and 50%), volume of applications (3, 5, and 7 L/m on beds), and soil application timing (30 and 120 days after planting); an untreated control was also included. Five months after planting, soil samples from each plot were collected to evaluate soil fertility. At harvest, disease incidence, nematode population densities in soil and crop yield were evaluated. In both experiments, there was no significant interaction between manipueira concentration and volume. An effect only of manipueira volume on the measured variables was observed. In the non-irrigated trial, the application of manipueira to soil reduced disease incidence and nematode population densities and increased fresh weight of yam tubers. In the irrigated trial, a positive effect was observed only at a volume of 5 L/m. In this treatment, total nematode population density was inversely related with soil Ca (r = -0.67, P < 0.017) and nematode incidence with soil Ca and Mg content (r = -0.71, P < 0.012). The volume of 5 L/m manipueira might be suitable for nematode management. A casca-preta-do-inhame causada pelos fitonematoides Scutellonema bradys, Pratylenchus coffeae e P. brachyurus tem limitado o rendimento da cultura do inhame (Dioscorea spp.) no Brasil. Assim, torna-se necessário o desenvolvimento de estratégias para reduzir as perdas provocadas por estes patógenos. O objetivo deste trabalho foi avaliar o efeito da aplicação de manipueira no manejo da casca-preta, em duas condições de plantio (sem ou com irrigação suplementar) e sobre as características do solo. Os experimentos foram instalados em áreas naturalmente infestadas, em delineamento experimental de blocos casualizados completos com quatro repetições, em esquema fatorial: concentrações de manipueira diluída em água (10, 25 e 50%), volumes de aplicação (3, 5 e 7 L/m sobre as leiras) aos 30 e 120 dias após o plantio, mais a testemunha. Para o experimento conduzido sob condições de irrigação, aos cinco meses após o plantio foram coletadas amostras de solo por parcela para avaliar a fertilidade do solo. Por ocasião da colheita foram avaliados a incidência da doença, população de nematoides no solo e rizóforos e o rendimento da cultura. Em ambos os experimentos, a análise de variância não apresentou interação significativa entre concentração e volume de manipueira. Observou-se apenas efeito de volume sobre as variáveis avaliadas. Em condições de sequeiro, a aplicação de manipueira ao solo reduziu a incidência da doença, a população de nematoides e também resultou em aumento da massa fresca dos rizóforos. Na área irrigada, o efeito positivo foi observado apenas com a aplicação do volume de 5 L/m. Nesta condição, a população total de nematoides mostrou uma relação inversa com o teor de Ca no solo (r = -0,67, P < 0,017), e a incidência da doença com a soma dos teores de Ca e Mg (r = -0,71, P < 0,012). O volume de 5 L/m de manipueira pode ser indicado para o manejo da doença. MenosDry rot disease caused by the plant-parasitic nematodes Scutellonema bradys, Pratylenchus coffeae, and P. brachyurus limits yam (Dioscorea spp.) yield in Brazil. As a result, the development of strategies to reduce agricultural losses caused by these plant-parasitic nematodes is required. The objective of this study was to evaluate the effect of cassava wastewater (manipueira) on the management of dry rot under two growing conditions (without or with supplemental irrigation) and on soil chemical characteristics. The following treatments were considered: manipueira concentrations diluted in water (10, 25, and 50%), volume of applications (3, 5, and 7 L/m on beds), and soil application timing (30 and 120 days after planting); an untreated control was also included. Five months after planting, soil samples from each plot were collected to evaluate soil fertility. At harvest, disease incidence, nematode population densities in soil and crop yield were evaluated. In both experiments, there was no significant interaction between manipueira concentration and volume. An effect only of manipueira volume on the measured variables was observed. In the non-irrigated trial, the application of manipueira to soil reduced disease incidence and nematode population densities and increased fresh weight of yam tubers. In the irrigated trial, a positive effect was observed only at a volume of 5 L/m. In this treatment, total nematode population density was inversely related with soil Ca (r = -0.6... Mostrar Tudo |
Thesagro: |
Doença de Planta; Inhame; Manipueira; Praga de Planta. |
Thesaurus NAL: |
Cassava; Plant diseases and disorders; Wastewater; Yams. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150346/1/Management-of-dry-rot-disease....pdf
|
Marc: |
LEADER 04391naa a2200289 a 4500 001 2150346 005 2022-12-26 008 2022 bl uuuu u00u1 u #d 100 1 $aFARIAS, S. P. 245 $aManagement of dry rot disease of uam with cassava wastewater.$h[electronic resource] 260 $c2022 520 $aDry rot disease caused by the plant-parasitic nematodes Scutellonema bradys, Pratylenchus coffeae, and P. brachyurus limits yam (Dioscorea spp.) yield in Brazil. As a result, the development of strategies to reduce agricultural losses caused by these plant-parasitic nematodes is required. The objective of this study was to evaluate the effect of cassava wastewater (manipueira) on the management of dry rot under two growing conditions (without or with supplemental irrigation) and on soil chemical characteristics. The following treatments were considered: manipueira concentrations diluted in water (10, 25, and 50%), volume of applications (3, 5, and 7 L/m on beds), and soil application timing (30 and 120 days after planting); an untreated control was also included. Five months after planting, soil samples from each plot were collected to evaluate soil fertility. At harvest, disease incidence, nematode population densities in soil and crop yield were evaluated. In both experiments, there was no significant interaction between manipueira concentration and volume. An effect only of manipueira volume on the measured variables was observed. In the non-irrigated trial, the application of manipueira to soil reduced disease incidence and nematode population densities and increased fresh weight of yam tubers. In the irrigated trial, a positive effect was observed only at a volume of 5 L/m. In this treatment, total nematode population density was inversely related with soil Ca (r = -0.67, P < 0.017) and nematode incidence with soil Ca and Mg content (r = -0.71, P < 0.012). The volume of 5 L/m manipueira might be suitable for nematode management. A casca-preta-do-inhame causada pelos fitonematoides Scutellonema bradys, Pratylenchus coffeae e P. brachyurus tem limitado o rendimento da cultura do inhame (Dioscorea spp.) no Brasil. Assim, torna-se necessário o desenvolvimento de estratégias para reduzir as perdas provocadas por estes patógenos. O objetivo deste trabalho foi avaliar o efeito da aplicação de manipueira no manejo da casca-preta, em duas condições de plantio (sem ou com irrigação suplementar) e sobre as características do solo. Os experimentos foram instalados em áreas naturalmente infestadas, em delineamento experimental de blocos casualizados completos com quatro repetições, em esquema fatorial: concentrações de manipueira diluída em água (10, 25 e 50%), volumes de aplicação (3, 5 e 7 L/m sobre as leiras) aos 30 e 120 dias após o plantio, mais a testemunha. Para o experimento conduzido sob condições de irrigação, aos cinco meses após o plantio foram coletadas amostras de solo por parcela para avaliar a fertilidade do solo. Por ocasião da colheita foram avaliados a incidência da doença, população de nematoides no solo e rizóforos e o rendimento da cultura. Em ambos os experimentos, a análise de variância não apresentou interação significativa entre concentração e volume de manipueira. Observou-se apenas efeito de volume sobre as variáveis avaliadas. Em condições de sequeiro, a aplicação de manipueira ao solo reduziu a incidência da doença, a população de nematoides e também resultou em aumento da massa fresca dos rizóforos. Na área irrigada, o efeito positivo foi observado apenas com a aplicação do volume de 5 L/m. Nesta condição, a população total de nematoides mostrou uma relação inversa com o teor de Ca no solo (r = -0,67, P < 0,017), e a incidência da doença com a soma dos teores de Ca e Mg (r = -0,71, P < 0,012). O volume de 5 L/m de manipueira pode ser indicado para o manejo da doença. 650 $aCassava 650 $aPlant diseases and disorders 650 $aWastewater 650 $aYams 650 $aDoença de Planta 650 $aInhame 650 $aManipueira 650 $aPraga de Planta 700 1 $aALMEIDA, A. V. D. L. 700 1 $aSANTOS, S. L. 700 1 $aMOURA FILHO, M. 700 1 $aGOMES, T. C. de A. 700 1 $aROCHA, F. S. 700 1 $aMUNIZ, M. F. S. 773 $tNematrópica$gv. 52, n. 1, 2022.
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