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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. F. dos; MENDONCA, L. C.; REIS, D. R. de L.; GUIMARAES, A. S.; LANGE, C. C.; RIBEIRO, J. B.; MACHADO, M. A.; BRITO, M. A. V. P. e. |
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Afiliação: |
Fernanda Fernandes dos Santos, UFJF; LETICIA CALDAS MENDONCA, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; CARLA CHRISTINE LANGE, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MARIA APARECIDA V PAIVA E BRITO, CNPGL. |
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Título: |
Presence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 99, n. 2, p. 1374-1382, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, erythromycin, sulfonamide, trimethoprim-sulfamethoxazole, and tetracycline. All S. aureus isolates were susceptible to oxacillin, whereas 47% (n = 43) of the CNS isolates were resistant. The CNS isolates showed a higher resistance to cephalothin, erythromycin, tetracycline, and gentamicin in comparison with S. aureus. The mecA gene was only detected in 10 CNS isolates, identified as Staphylococcus epidermidis, and classified into 3 pulsotypes (A, B, and C) and 4 subtypes (A1, B1, B2, and B3). Among the isolates with an oxacillin resistance phenotype, 12 were positive for the blaZ gene, and 9 of them were mecA-positive. Two of the oxacillin-resistant isolates amplified the mecA homolog gene of Staphylococcus sciuri and none amplified mecC. Three SCCmec types, I, IV, and V, were found. Our results suggest that Staphylococcus epidermidis can be a reservoir for mecA for other Staphylococcus species. Studies investigating the molecular and phenotypic profile of antimicrobial resistance in staphylococcal species should be performed for controlling the spread of resistance and the selection of appropriate therapeutic measures. MenosAbstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, ery... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial resistance; MecA; Methicillin-resistant staphylococci; SCCmec. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03425naa a2200253 a 4500
001 2061479
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. F. dos
245 $aPresence of mecA-positive multidrug-resistant Staphylococcus epidermidis in bovine milk samples in Brazil.$h[electronic resource]
260 $c2016
520 $aAbstract Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method. In addition to the Etest, the phenotypic characterization of oxacillin resistance was tested using the cefoxitin disk diffusion test. All isolates were screened by PCR to detect the mecA gene in 2 different regions of the gene. The isolates with an oxacillin minimum inhibitory concentration ≥0.5 µg/mL or resistant to cefoxitin were identified by sequencing a 536-bp fragment of the 16S rRNA gene. This group of isolates was also evaluated for the presence of blaZ and mecC genes. Molecular analysis of the mecA gene was carried out by typing of the staphylococcal cassette chromosome mec (SCCmec). The relatedness of the mecA-positive isolates was evaluated by macrorestriction of chromosomal DNA followed by pulsed-field gel electrophoresis. With the exception of penicillin and oxacillin, 86% of the isolates showed susceptibility to cephalothin, gentamicin, erythromycin, sulfonamide, trimethoprim-sulfamethoxazole, and tetracycline. All S. aureus isolates were susceptible to oxacillin, whereas 47% (n = 43) of the CNS isolates were resistant. The CNS isolates showed a higher resistance to cephalothin, erythromycin, tetracycline, and gentamicin in comparison with S. aureus. The mecA gene was only detected in 10 CNS isolates, identified as Staphylococcus epidermidis, and classified into 3 pulsotypes (A, B, and C) and 4 subtypes (A1, B1, B2, and B3). Among the isolates with an oxacillin resistance phenotype, 12 were positive for the blaZ gene, and 9 of them were mecA-positive. Two of the oxacillin-resistant isolates amplified the mecA homolog gene of Staphylococcus sciuri and none amplified mecC. Three SCCmec types, I, IV, and V, were found. Our results suggest that Staphylococcus epidermidis can be a reservoir for mecA for other Staphylococcus species. Studies investigating the molecular and phenotypic profile of antimicrobial resistance in staphylococcal species should be performed for controlling the spread of resistance and the selection of appropriate therapeutic measures.
653 $aAntimicrobial resistance
653 $aMecA
653 $aMethicillin-resistant staphylococci
653 $aSCCmec
700 1 $aMENDONCA, L. C.
700 1 $aREIS, D. R. de L.
700 1 $aGUIMARAES, A. S.
700 1 $aLANGE, C. C.
700 1 $aRIBEIRO, J. B.
700 1 $aMACHADO, M. A.
700 1 $aBRITO, M. A. V. P. e
773 $tJournal of Dairy Science$gv. 99, n. 2, p. 1374-1382, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
03/12/2014 |
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Data da última atualização: |
03/10/2016 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
RICHETTI, A.; CECCON, G. |
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Afiliação: |
ALCEU RICHETTI, CPAO; GESSI CECCON, CPAO. |
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Título: |
Viabilidade econômica da cultura do milho safrinha, 2015, em Mato Grosso do Sul. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Dourados: Embrapa Agropecuária Oeste, 2014. |
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Páginas: |
10 p. |
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Série: |
(Embrapa Agropecuária Oeste. Comunicado técnico, 196). |
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Idioma: |
Português |
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Thesagro: |
Milho. |
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Thesaurus NAL: |
corn. |
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Categoria do assunto: |
E Economia e Indústria Agrícola |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112911/1/COT2014196-final-1.pdf
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Marc: |
LEADER 00497nam a2200157 a 4500
001 2001443
005 2016-10-03
008 2014 bl uuuu u0uu1 u #d
100 1 $aRICHETTI, A.
245 $aViabilidade econômica da cultura do milho safrinha, 2015, em Mato Grosso do Sul.$h[electronic resource]
260 $aDourados: Embrapa Agropecuária Oeste$c2014
300 $a10 p.
490 $a(Embrapa Agropecuária Oeste. Comunicado técnico, 196).
650 $acorn
650 $aMilho
700 1 $aCECCON, G.
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Embrapa Agropecuária Oeste (CPAO) |
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