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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/10/2010 |
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Data da última atualização: |
31/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMA NETO, I. V.; RIBEIRO, R. A.; HUNGRIA, M. |
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Afiliação: |
ILMARA VAROTTO ROMA NETO, UEL; RENAN AUGUSTO RIBEIRO, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Genetic diversity of elite rhizobial strains of subtropical and tropical legumes based on the 16S rRNA and glnII genes. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, Oxford, v. 26, n. 7, p. 1291-1302, jul. 2010. |
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DOI: |
10.1007/s11274-009-0300-3 |
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Idioma: |
Português |
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Conteúdo: |
Biodiversity of diazotrophic symbiotic bacteria in the tropics is a valuable but still poorly studied resource. The objective of this study was to determine if a second housekeeping gene, glnII, in addition to the 16S rRNA, can be employed to improve the knowledge about taxonomy and phylogeny of rhizobia. Twenty-three elite rhizobial strains, very effective in fixing nitrogen with twenty-one herbal and woody legumes (including species from fourteen tribes in the three subfamilies of the family Leguminosae) were selected for this study; all strains are used as commercial inoculants in Brazil. Complete sequences of the 16S rRNA and partial sequences (480 bp) of the glnII gene were obtained. The same primers and amplification conditions were successful for sequencing the glnII genes of bacteria belonging to five different rhizobial genera?Bradyrhizobium, Mesorhizobium, Methylobacterium, Rhizobium, Sinorhizobium)?positioned in distantly related branches. The analysis of the concatenated genes (16S rRNA ? glnII) considerably improved information about phylogeny and taxonomy of rhizobia in comparison to the single analysis of the 16S rRNA. Nine strains might belong to new species. The complementary analysis of the glnII gene was successful with all strains and improved the phylogenetic clustering and clarified the taxonomic position of several strains. The strategy of including the analysis of glnII, in addition to the 16S rRNA, is cost- and time- effective for the characterization of large rhizobial culture collections or in surveys of many isolates. MenosBiodiversity of diazotrophic symbiotic bacteria in the tropics is a valuable but still poorly studied resource. The objective of this study was to determine if a second housekeeping gene, glnII, in addition to the 16S rRNA, can be employed to improve the knowledge about taxonomy and phylogeny of rhizobia. Twenty-three elite rhizobial strains, very effective in fixing nitrogen with twenty-one herbal and woody legumes (including species from fourteen tribes in the three subfamilies of the family Leguminosae) were selected for this study; all strains are used as commercial inoculants in Brazil. Complete sequences of the 16S rRNA and partial sequences (480 bp) of the glnII gene were obtained. The same primers and amplification conditions were successful for sequencing the glnII genes of bacteria belonging to five different rhizobial genera?Bradyrhizobium, Mesorhizobium, Methylobacterium, Rhizobium, Sinorhizobium)?positioned in distantly related branches. The analysis of the concatenated genes (16S rRNA ? glnII) considerably improved information about phylogeny and taxonomy of rhizobia in comparison to the single analysis of the 16S rRNA. Nine strains might belong to new species. The complementary analysis of the glnII gene was successful with all strains and improved the phylogenetic clustering and clarified the taxonomic position of several strains. The strategy of including the analysis of glnII, in addition to the 16S rRNA, is cost- and time- effective for the characterizatio... Mostrar Tudo |
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Palavras-Chave: |
Nitrogem fixation. |
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Thesagro: |
Fixacao de nitrogenio; Gene; Leguminosa tropical; RNA. |
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Thesaurus Nal: |
Fabaceae; Genes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02313naa a2200241 a 4500
001 1864553
005 2017-07-31
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s11274-009-0300-3$2DOI
100 1 $aROMA NETO, I. V.
245 $aGenetic diversity of elite rhizobial strains of subtropical and tropical legumes based on the 16S rRNA and glnII genes.
260 $c2010
520 $aBiodiversity of diazotrophic symbiotic bacteria in the tropics is a valuable but still poorly studied resource. The objective of this study was to determine if a second housekeeping gene, glnII, in addition to the 16S rRNA, can be employed to improve the knowledge about taxonomy and phylogeny of rhizobia. Twenty-three elite rhizobial strains, very effective in fixing nitrogen with twenty-one herbal and woody legumes (including species from fourteen tribes in the three subfamilies of the family Leguminosae) were selected for this study; all strains are used as commercial inoculants in Brazil. Complete sequences of the 16S rRNA and partial sequences (480 bp) of the glnII gene were obtained. The same primers and amplification conditions were successful for sequencing the glnII genes of bacteria belonging to five different rhizobial genera?Bradyrhizobium, Mesorhizobium, Methylobacterium, Rhizobium, Sinorhizobium)?positioned in distantly related branches. The analysis of the concatenated genes (16S rRNA ? glnII) considerably improved information about phylogeny and taxonomy of rhizobia in comparison to the single analysis of the 16S rRNA. Nine strains might belong to new species. The complementary analysis of the glnII gene was successful with all strains and improved the phylogenetic clustering and clarified the taxonomic position of several strains. The strategy of including the analysis of glnII, in addition to the 16S rRNA, is cost- and time- effective for the characterization of large rhizobial culture collections or in surveys of many isolates.
650 $aFabaceae
650 $aGenes
650 $aFixacao de nitrogenio
650 $aGene
650 $aLeguminosa tropical
650 $aRNA
653 $aNitrogem fixation
700 1 $aRIBEIRO, R. A.
700 1 $aHUNGRIA, M.
773 $tWorld Journal of Microbiology and Biotechnology, Oxford$gv. 26, n. 7, p. 1291-1302, jul. 2010.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
25/09/2001 |
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Data da última atualização: |
30/08/2011 |
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Tipo da produção científica: |
Circular Técnica |
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Autoria: |
BARBO, C. V. S.; CARNIELLI, A.; POTTKER, D.; SALVADORI, J. R.; SILVA, C. M. da; SONEGO, O. R.; FERNANDES, F. M. |
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Título: |
Recomendações técnicas para a cultura da soja: região da Grande Dourados - safra 1980/81. |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
Dourados: EMBRAPA-UEPAE Dourados, 1980. |
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Páginas: |
53 p. |
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Série: |
(EMBRAPA-UEPAE Dourados. Circular técnica, 2). |
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Idioma: |
Português |
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Conteúdo: |
Solos: amostragem, conservacao, correcao e adubacao; Epoca de semeadura; Espacamento e densidade e caracteristicas; Cultivares de soja e caracteristicas; Controle de ervas daninhas; Manejo de pragas; Tratamento quimico de sementes; Controle de doencas; Colheita. |
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Thesagro: |
Erva Daninha; Praga de Planta; Soja; Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40852/1/CT-02.80.pdf
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Marc: |
LEADER 00973nam a2200253 a 4500
001 1255176
005 2011-08-30
008 1980 bl uuuu 00u1 u #d
100 1 $aBARBO, C. V. S.
245 $aRecomendações técnicas para a cultura da soja$bregião da Grande Dourados - safra 1980/81.
260 $aDourados: EMBRAPA-UEPAE Dourados$c1980
300 $a53 p.
490 $a(EMBRAPA-UEPAE Dourados. Circular técnica, 2).
520 $aSolos: amostragem, conservacao, correcao e adubacao; Epoca de semeadura; Espacamento e densidade e caracteristicas; Cultivares de soja e caracteristicas; Controle de ervas daninhas; Manejo de pragas; Tratamento quimico de sementes; Controle de doencas; Colheita.
650 $aErva Daninha
650 $aPraga de Planta
650 $aSoja
650 $aSolo
700 1 $aCARNIELLI, A.
700 1 $aPOTTKER, D.
700 1 $aSALVADORI, J. R.
700 1 $aSILVA, C. M. da
700 1 $aSONEGO, O. R.
700 1 $aFERNANDES, F. M.
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