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Biblioteca(s):  Embrapa Caprinos e Ovinos.
Data corrente:  21/05/2024
Data da última atualização:  21/05/2024
Autoria:  FOSTER, P. M. D.; GREEN, M. L.
Afiliação:  MARGARET L. GREEN.
Título:  A quantitative gel-filtration method for analysis of the proteinaceous fraction of Cheddar cheese.
Ano de publicação:  1974
Fonte/Imprenta:  Journal of Dairy Research, v. 41, n. 2, p. 259-268, Jun. 1974.
DOI:  https://doi.org/10.1017/S0022029900019671
Idioma:  Inglês
Conteúdo:  Methods are described for the solubilization of the nitrogenous portion of cheese in a dissociating solvent system and separation of the solution into fractions on a Sephadex G-100 column under conditions where the proteins and peptides would be expected to be split into monomeric units. The E280 measurement of the fractions, expressed as a percentage of the total material eluted, followed a reproducible pattern, both with duplicate runs on the same sample and comparative runs on non-identical samples of the same type. With cheese curd, a small peak of undissociated material was obtained at the void volume of the column; this was followed by a main peak and a minor third peak, with a small quantity of material being eluted at larger volumes. A qualitatively similar pattern was obtained with 43-day-old cheese as the sample, but the main peak was smaller, the third peak was larger, and a relatively larger amount of material was eluted at greater volumes. There was only partial separation of the proteins on the column; the main peak contained β- and αs-caseins, their larger breakdown products and some para-κ-casein, and the third peak contained smaller breakdown products of the main casein fractions and para-κ-casein. Although the resolution was considerably inferior to that obtained by gel electrophoresis, the method has the advantage over this and other methods in that it gives a quantitative analysis of the sample based on molecular size. Thus, it is suggested that it may fi... Mostrar Tudo
Thesaurus Nal:  Casein; Cheese milk; Cheeses; Milk proteins; Peptides.
Categoria do assunto:  L Ciência Animal e Produtos de Origem Animal
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Caprinos e Ovinos (CNPC)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CNPC40819 - 1ADDAP - DD
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