| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
|
Data corrente: |
06/02/2018 |
|
Data da última atualização: |
06/02/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SOUSA, N. L.; CABRAL, G. B.; VIEIRA, P. M.; BALDONI, A. B.; ARAGAO, F. J. L. |
|
Afiliação: |
NATÁLIA L. SOUSA, UNB; GLAUCIA BARBOSA CABRAL, Cenargen; PABLINE M. VIEIRA, INSTITUTO FEDERAL GOIANO; AISY BOTEGA BALDONI TARDIN, CPAMT; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
|
Título: |
Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Scientific Reports, v. .7, n. 1, 2017. (Open). |
|
DOI: |
10.1038/s41598-017-15636-7 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society. |
|
Palavras-Chave: |
Toxic ribosome-inactivating. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01828naa a2200193 a 4500
001 2087235
005 2018-02-06
008 2017 bl --- 0-- u #d
024 7 $a10.1038/s41598-017-15636-7$2DOI
100 1 $aSOUSA, N. L.
245 $aBio-detoxification of ricin in castor bean (Ricinus communis L.) seeds.$h[electronic resource]
260 $c2017
520 $aRicin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society.
653 $aToxic ribosome-inactivating
700 1 $aCABRAL, G. B.
700 1 $aVIEIRA, P. M.
700 1 $aBALDONI, A. B.
700 1 $aARAGAO, F. J. L.
773 $tScientific Reports$gv. .7, n. 1, 2017. (Open).
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrossilvipastoril. Para informações adicionais entre em contato com cpamt.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
|
Data corrente: |
19/01/2018 |
|
Data da última atualização: |
19/01/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
LOPES, T. R.; ZOLIN, C. A.; PRADO, G. do; PAULINO, J.; ALMEIDA, F. T. de. |
|
Afiliação: |
TÁRCIO R. LOPES, UFMT-SINOP; CORNELIO ALBERTO ZOLIN, CPAMT; GIULIANI DO PRADO, UEM; JANAÍNA PAULINO, UFMT-SINOP; FREDERICO T. DE ALMEIDA, UFMT-SINOP. |
|
Título: |
Regionalization of maximum and minimum flow in the teles pires basin, Brazil. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Engenharia Agrícola, v. 37, n. 1, p. 54-63, 2017. |
|
Idioma: |
Inglês |
|
Conteúdo: |
In a watershed there is space and climate variability within its catchment area, causing changes in water qualitative and quantitative characteristics. Thus, the hydrological data collected from a specific basin may be extrapolated to neighboring basins with similar physical characteristics, using techniques of hydrological regionalization. This study developed the flow regionalization in the Teles Pires Basin, using historic series and probabilistic models to verify the data adherence through the regression equations adjustment according to the hydrologically homogeneous regions. The linear and power regression models were tested, employing physical and climatic characteristics and then the best adjustments were selected based on coefficients of determination, adjusted coefficient of determination, significance by F-test and small number of variables in the equation. The physical characteristics of the basin, drainage area and mainstream length, and the climatic variable, annual total precipitation showed higher adjustments. These equations can provide subsidy for decision making in the process of water resource management. |
|
Palavras-Chave: |
Hydrologic. |
|
Thesaurus NAL: |
Resource management; Water resources. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 01730naa a2200205 a 4500
001 2085927
005 2018-01-19
008 2017 bl uuuu u00u1 u #d
100 1 $aLOPES, T. R.
245 $aRegionalization of maximum and minimum flow in the teles pires basin, Brazil.$h[electronic resource]
260 $c2017
520 $aIn a watershed there is space and climate variability within its catchment area, causing changes in water qualitative and quantitative characteristics. Thus, the hydrological data collected from a specific basin may be extrapolated to neighboring basins with similar physical characteristics, using techniques of hydrological regionalization. This study developed the flow regionalization in the Teles Pires Basin, using historic series and probabilistic models to verify the data adherence through the regression equations adjustment according to the hydrologically homogeneous regions. The linear and power regression models were tested, employing physical and climatic characteristics and then the best adjustments were selected based on coefficients of determination, adjusted coefficient of determination, significance by F-test and small number of variables in the equation. The physical characteristics of the basin, drainage area and mainstream length, and the climatic variable, annual total precipitation showed higher adjustments. These equations can provide subsidy for decision making in the process of water resource management.
650 $aResource management
650 $aWater resources
653 $aHydrologic
700 1 $aZOLIN, C. A.
700 1 $aPRADO, G. do
700 1 $aPAULINO, J.
700 1 $aALMEIDA, F. T. de
773 $tEngenharia Agrícola$gv. 37, n. 1, p. 54-63, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
