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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/09/2003 |
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Data da última atualização: |
03/09/2003 |
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Autoria: |
ALMEIDA, A. M. R.; ABDELNOOR, R. V.; ARIAS, C. A. A.; CARVALHO, V. P. JACOUD FILHO, D. S.; MARIN, S. R. R.; BENATO, L. C.; PINTO, M. C.; CARVALHO, C. G. P. |
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Título: |
Genotypic diversity among brazilian isolates of Macrophomina phaseolina revealed by RAPD. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, v. 28, n. 3, p. 279-285, maio/jun. 2003. |
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Idioma: |
Português |
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Conteúdo: |
Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In another study the genetic similarity was evaluated among isolates from different hosts [soybean, sorghum (Sorghum bicolor), sunflower (Helianthus annuus), cowpea (Vigna unguiculata), corn (Zea mays) and wheat (Triticum aestivum)] as well as two soil samples from native areas. Results showed that more divergent isolates originated from areas with a single crop. Isolates from areas with crop rotation were less divergent, showing high similarity values and consequently formed the largest group. Amplification of the ITS region using primers ITS1 and ITS4 produced only one DNA fragment of 620 bp. None of the isolates were differentiated through PCR-RFLP. Our results demonstrated genetic variability among Brazilian isolates of M. phaseolina and showed that one single root can harbor more than one haplotype. Moreover, cultivation with crop rotation tends to induce less specialization of the pathogen isolates. Knowledge of this variation may be useful in screening soybean genotypes for resistance to charcoal rot. MenosMacrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In another study the genetic similarity was evaluated among isolates from different hosts [soybean, sorghum (Sorghum bicolor), sunflower (Helianthus annuus), cowpea (Vigna unguiculata), corn (Zea mays) and wheat (Triticum aestivum)] as well as two soil samples from native areas. Results showed that more divergent isolates originated from areas with a single crop. Isolates from areas with crop rotation were less divergent, showing high similarity values and consequently formed the largest group. Amplification of the ITS region using primers ITS1 and ITS4 produced only one DNA fragment of 620 bp. None of the isolates were differentiated through PCR-RFLP. Our results demonstrated genetic variability among Brazilian isolates of M. ... Mostrar Tudo |
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Categoria do assunto: |
-- |
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URL: |
https://www.scielo.br/pdf/fb/v28n3/a09v28n3.pdf
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Marc: |
LEADER 02422naa a2200205 a 4500
001 1460324
005 2003-09-03
008 2003 bl uuuu u00u1 u #d
100 1 $aALMEIDA, A. M. R.
245 $aGenotypic diversity among brazilian isolates of Macrophomina phaseolina revealed by RAPD.
260 $c2003
520 $aMacrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In another study the genetic similarity was evaluated among isolates from different hosts [soybean, sorghum (Sorghum bicolor), sunflower (Helianthus annuus), cowpea (Vigna unguiculata), corn (Zea mays) and wheat (Triticum aestivum)] as well as two soil samples from native areas. Results showed that more divergent isolates originated from areas with a single crop. Isolates from areas with crop rotation were less divergent, showing high similarity values and consequently formed the largest group. Amplification of the ITS region using primers ITS1 and ITS4 produced only one DNA fragment of 620 bp. None of the isolates were differentiated through PCR-RFLP. Our results demonstrated genetic variability among Brazilian isolates of M. phaseolina and showed that one single root can harbor more than one haplotype. Moreover, cultivation with crop rotation tends to induce less specialization of the pathogen isolates. Knowledge of this variation may be useful in screening soybean genotypes for resistance to charcoal rot.
700 1 $aABDELNOOR, R. V.
700 1 $aARIAS, C. A. A.
700 1 $aCARVALHO, V. P. JACOUD FILHO, D. S.
700 1 $aMARIN, S. R. R.
700 1 $aBENATO, L. C.
700 1 $aPINTO, M. C.
700 1 $aCARVALHO, C. G. P.
773 $tFitopatologia Brasileira, Brasília$gv. 28, n. 3, p. 279-285, maio/jun. 2003.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/09/2021 |
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Data da última atualização: |
07/12/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NUCCI, A. DA S.; NICIURA, S. C. M.; FELIPELLI, G.; OKINO, C. H.; MALAGO JUNIOR, W. |
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Afiliação: |
ALESSANDRA DA SILVA NUCCI, UNICEP; SIMONE CRISTINA MEO NICIURA, CPPSE; GUSTAVO FELIPPELLI, FAPESP; CINTIA HIROMI OKINO, CPPSE; WILSON MALAGO JUNIOR, CPPSE. |
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Título: |
Genotipagem por PCR de deleção no éxon 11 do gene mptl-1 associada à resistência ao monepantel em Haemonchus contortus. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2021. |
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Páginas: |
p.23. |
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Série: |
(Embrapa Pecuária Sudeste. Documentos, 140). |
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ISSN: |
1980-6841 |
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Idioma: |
Português |
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Conteúdo: |
As infecções por nematoides gastrointestinais, principalmente as causadas por Haemonchus contortus, são responsáveis por grandes perdas econômicas na produção ovina devido ao parasitismo e à resistência antihelmíntica. |
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Palavras-Chave: |
Nematoides gastrintestinais; PAGE; QPCR. |
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Thesagro: |
Polimorfismo; Resistência. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225954/1/GenotipagemPCRDelecao.pdf
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Marc: |
LEADER 01120nam a2200253 a 4500
001 2134336
005 2021-12-07
008 2021 bl uuuu u00u1 u #d
022 $a1980-6841
100 1 $aNUCCI, A. DA S.
245 $aGenotipagem por PCR de deleção no éxon 11 do gene mptl-1 associada à resistência ao monepantel em Haemonchus contortus.$h[electronic resource]
260 $aIn: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação$c2021
300 $ap.23.
490 $a(Embrapa Pecuária Sudeste. Documentos, 140).
520 $aAs infecções por nematoides gastrointestinais, principalmente as causadas por Haemonchus contortus, são responsáveis por grandes perdas econômicas na produção ovina devido ao parasitismo e à resistência antihelmíntica.
650 $aPolimorfismo
650 $aResistência
653 $aNematoides gastrintestinais
653 $aPAGE
653 $aQPCR
700 1 $aNICIURA, S. C. M.
700 1 $aFELIPELLI, G.
700 1 $aOKINO, C. H.
700 1 $aMALAGO JUNIOR, W.
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Embrapa Pecuária Sudeste (CPPSE) |
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