Data/hora: 07/06/2024 02:56:49

Biblioteca(s): | Embrapa Florestas.
Data corrente: | 15/09/2008
Data da última atualização: | 15/09/2008
Autoria: | GOEDE, R. de; HOFFLAND, E.; HELDER, H.; MULDER, C.; POSTHUMA, L.
Título: | GM-crop impact assessment on soil ecosystems by DNA bare-code based monitoring of nematode communities (ERGONema).
Ano de publicação: | 2008
Fonte/Imprenta: | In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
Idioma: | Inglês

Conteúdo:

Based on the recommendation of the Dutch Advisory commission on Genetic Modification
(Cogem) the Dutch government initiated a research program (Ecology Regarding Gene-modified
Organisms; ERGO) on the better ecological understanding of non-target effects of GM-crops on
soil ecosystem functioning. We will study such effects for the interactions between GM-crops,
nematodes and soil fertility in our ERGONema project, starting in 2008. The closest interactions
between a genetically modified (GM) crop and its environment take place in the rhizosphere
and during litter decomposition. A potentially relevant hazard scenario is that GM crops affect
these organisms, with consequences for soil fertility. Nematodes constitute a diverse group
within the soil fauna, feeding on bacteria, fungi, other nematodes and plant-roots. This intimate
feeding relationship with all major players in direct contact with plant residues implies that GMcrop
effects in the rhizosphere and on the decomposer community will be reflected in the nematode
community. However, nematodes look alike very much, and for large scale ecological analysis
of communities non-morphological characteristics should be used. DNA barcodes are being
developed for individual nematode families and quantitative PCR can be used to monitor changes
in the nematode community at a large scale with unprecedented resolution. The ERGONema
project aims to deliver guidance for a validated Ecological Risk Assessment (ERA) method to
evaluate potential risks of GM crops on soil biodiversity and fertility, using a quick, accurate and
affordable nematode DNA-based tool.
Activities:
? Establishment of the response window of a high impact, non-GM crop (i.e. positive control) on
nematode communities, using newly developed DNA-barcodes;
? Establishment of the response window of a high impact, non-GM crop on soil fertility;
? Determine the relationship between changes in nematode communities and soil fertility;
? Integration of the scientific findings of activities 1 and 2 into a draft ERA-guidance.
? Validate the newly developed procedure in GM crops under field conditions.

Categoria do assunto: | --
Registro original: | Embrapa Florestas (CNPF)
Exemplares: | CNPF - (43563-1) - UPE - PL - -- - CD0305 -  -  - DIS