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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M. |
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Afiliação: |
Michele Munk, UFJF; Luiz O. Ladeira, UFMG; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nádia R. B. Raposo, UFJF; Raquel V. Serapião, PESAGRO; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; Saulo R. Silva; Jaqueline S. Soares, UFOP; Ado Jorio, UFMG; HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Nature, Scientific Reports, v. 6, article 33588, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos. |
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Palavras-Chave: |
Gene delivery; Pellucid zone. |
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Thesaurus Nal: |
in vitro fertilization. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155955/1/Cnpgl-2016-Nature-Efficient.pdf
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Marc: |
LEADER 01768naa a2200277 a 4500
001 2064560
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aMUNK, M.
245 $aEfficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.$h[electronic resource]
260 $c2016
520 $aThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
650 $ain vitro fertilization
653 $aGene delivery
653 $aPellucid zone
700 1 $aLADEIRA, L. O.
700 1 $aCARVALHO, B. C. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aRAPOSO, N. R. B.
700 1 $aSERAPIÁO, R. V.
700 1 $aQUINTAO, C. C. R.
700 1 $aSILVA, S. R.
700 1 $aSOARES, J. S.
700 1 $aJORIO, A.
700 1 $aBRANDAO, H. de M.
773 $tNature, Scientific Reports$gv. 6, article 33588, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
30/03/1998 |
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Data da última atualização: |
28/12/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MACEDO, J. L. V. de; MORAES, C. R. de A.; MOTA, A. M. |
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Afiliação: |
JEFERSON LUIS VASCONCELOS DE MACEDO, CPAA. |
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Título: |
Development and production of peach palm (Bactris gasipaes) for palm heart in different polyculture systems. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: SHIFT-WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS, 1998. |
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Páginas: |
p. C9. |
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Idioma: |
Inglês |
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Conteúdo: |
The development and yield of peach palm was evaluated in two agroforestry systems. |
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Palavras-Chave: |
Agrofloresta; Amazonas; Brasil. |
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Thesagro: |
Bactris Gasipaes; Cultivo Multiplo; Pupunha. |
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Thesaurus NAL: |
agroforestry; multiple cropping. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/124255/1/C9.pdf
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Marc: |
LEADER 00824nam a2200241 a 4500
001 1667706
005 2018-12-28
008 1998 bl uuuu u00u1 u #d
100 1 $aMACEDO, J. L. V. de
245 $aDevelopment and production of peach palm (Bactris gasipaes) for palm heart in different polyculture systems.
260 $aIn: SHIFT-WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS$c1998
300 $ap. C9.
520 $aThe development and yield of peach palm was evaluated in two agroforestry systems.
650 $aagroforestry
650 $amultiple cropping
650 $aBactris Gasipaes
650 $aCultivo Multiplo
650 $aPupunha
653 $aAgrofloresta
653 $aAmazonas
653 $aBrasil
700 1 $aMORAES, C. R. de A.
700 1 $aMOTA, A. M.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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