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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
04/11/1992 |
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Data da última atualização: |
04/11/1992 |
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Autoria: |
CUNNINGHAM, S.; KOLLMEYER, W. D.; STACEY, G. |
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Título: |
Chemical control of interstrain competition for soybean nodulation by Bradyrhizobium japonicum. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v.57, n.7, p.1886-1892, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Previous research has shown that a significant limitation to the agricultural use of improved rhizobial inoculant strains is competition from the indigenous soil population. In this work, we sought to test whether chemical inhibitors of flavonoid-induced nod gene expression in Bradyrhizobium japonicum could be identified and utilized to affect interstrain competition for nodulation of soybeans. Approximately 1,000 structural and functional analogs of the known, natural inducers of nod gene expression were tested on six strains of B. japonicum containing a nodY-lacZ fusion. We successfully identified effective inhibitors on nodY expression. The addition of the inhibitor 7-hydroxy-5-methylflavone significantly inhibited nodulation by a sensitive strain and could be used to effectively manipulate the competition between strains for soybean nodulation. However, this work also uncovered significant limitantions for the practical use of this methodology. For example, despite the almost universal induction response to the identified natural inducers, there was a wide variability among strains in their response to any specific inhibitor. Given this unexpected variability, the cost of registration of an agronomic chemical, and the potential for the development of resistant field populations, it is unlikely that chemical inhibitors can be successfully applied to a field situation. |
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Palavras-Chave: |
Competitivenes; Competitividade; Ecologia microbiana; Nod genes; Soil microbiology; Soybean. |
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Thesagro: |
Bradyrhizobium Japonicum; Glycine Max; Microbiologia; Nodulação; Soja; Solo. |
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Thesaurus Nal: |
microbial ecology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02228naa a2200301 a 4500
001 1457520
005 1992-11-04
008 1991 bl --- 0-- u #d
100 1 $aCUNNINGHAM, S.
245 $aChemical control of interstrain competition for soybean nodulation by Bradyrhizobium japonicum.
260 $c1991
520 $aPrevious research has shown that a significant limitation to the agricultural use of improved rhizobial inoculant strains is competition from the indigenous soil population. In this work, we sought to test whether chemical inhibitors of flavonoid-induced nod gene expression in Bradyrhizobium japonicum could be identified and utilized to affect interstrain competition for nodulation of soybeans. Approximately 1,000 structural and functional analogs of the known, natural inducers of nod gene expression were tested on six strains of B. japonicum containing a nodY-lacZ fusion. We successfully identified effective inhibitors on nodY expression. The addition of the inhibitor 7-hydroxy-5-methylflavone significantly inhibited nodulation by a sensitive strain and could be used to effectively manipulate the competition between strains for soybean nodulation. However, this work also uncovered significant limitantions for the practical use of this methodology. For example, despite the almost universal induction response to the identified natural inducers, there was a wide variability among strains in their response to any specific inhibitor. Given this unexpected variability, the cost of registration of an agronomic chemical, and the potential for the development of resistant field populations, it is unlikely that chemical inhibitors can be successfully applied to a field situation.
650 $amicrobial ecology
650 $aBradyrhizobium Japonicum
650 $aGlycine Max
650 $aMicrobiologia
650 $aNodulação
650 $aSoja
650 $aSolo
653 $aCompetitivenes
653 $aCompetitividade
653 $aEcologia microbiana
653 $aNod genes
653 $aSoil microbiology
653 $aSoybean
700 1 $aKOLLMEYER, W. D.
700 1 $aSTACEY, G.
773 $tApplied and Environmental Microbiology$gv.57, n.7, p.1886-1892, 1991.
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Registro original: |
Embrapa Soja (CNPSO) |
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