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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/09/2017 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
HELENA FABIANA REIS DE ALMEIDA SARAIVA, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, UNIVERSIDADE FEDERAL FLUMINENSE; PEDRO HENRIQUE NICOLAU PINTO, UNIVERSIDADE FEDERAL FLUMINENSE; LILIAN SANTOS RIBEIRO, UNIVERSIDADE FEDERAL FLUMINENSE; CLARA SLADE OLIVEIRA, CNPGL; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, Jan. 2018. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2017.09.022 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control... Mostrar Tudo |
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Palavras-Chave: |
Animal embryos; ATP cycle; Drug effects; Mitochondrial genetics; Santa ines; Survival. |
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Thesagro: |
Ovino. |
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Thesaurus Nal: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; oocytes; Sheep; Vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03336naa a2200433 a 4500
001 2076472
005 2025-01-10
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2017.09.022$2DOI
100 1 $aSARAIVA, H. F. R. de A.
245 $al-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression.$h[electronic resource]
260 $c2017
520 $aAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
650 $aCarnitine
650 $aCell culture
650 $aCryopreservation
650 $aEwes
650 $aGene expression
650 $aIn vitro culture
650 $aoocytes
650 $aSheep
650 $aVitrification
650 $aOvino
653 $aAnimal embryos
653 $aATP cycle
653 $aDrug effects
653 $aMitochondrial genetics
653 $aSanta ines
653 $aSurvival
700 1 $aBATISTA, R. I. T. P.
700 1 $aALFRADIQUE, V. A. P.
700 1 $aPINTO, P. H. N.
700 1 $aRIBEIRO, L. S.
700 1 $aOLIVEIRA, C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tTheriogenology$gv. 105, p. 150-157, Jan. 2018.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 11 | |
| 2. |  | HERMANN, K. L.; LOVATEL, A.; HELM, C. V.; LIMA, E. A. de; TAVARES, L. B. B. Avaliação da densidade micelial e da atividade de Manganês Peroxidase (MnP) em fungos basidiomicetos. Dynamis Revista Tecno-Científica, v. 16, n. 2, p. 40, 2010. Resumos da 4a. Mostra Integrada de Ensino, Pesquisa e Extensão, Blumenau, 2010. MIPE.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 3. | | HERMANN, K. L.; TAVARES, L. B. B.; COSTA, A.; HELM, C. V.; LIMA, E. A. de. Avaliação da expressão de ativos fúngicos para aplicação em creme despigmentante para tratamento de melasmas. Dynamis Revista Tecno-Científica, Blumenau, v. 17, n. 1, p. 77, 2011. Edição dos resumos da 5ª Mostra Integrada de Ensino, Pesquisa e Extensão, Blumenau, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 4. | | COSTA, A.; HERMANN, K. L.; HELM, C. V.; TAVARES, L. B. B. Produção de ativos fúngicos com potencial para aplicação em creme despigmentante para tratamento de melasma. Dynamis Revista Tecno-Científica, v. 16, n. 2, p. 42, 2010. Resumos da 4a. Mostra Integrada de Ensino, Pesquisa e Extensão, Blumenau, 2010. MIPE.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 6. | | HERMANN, K. L.; TAVARES, L. B. B.; COSTA, A.; LIMA, E. A. de; HELM, C. V. Expressão de manganês peroxidase por L. edodes e L. boryana em fermentação em estado sólido e sistema submerso. Dynamis Revista Tecno-Científica, Blumenau, v. 17, n. 1, p. 88, 2011. Edição dos resumos da 5ª Mostra Integrada de Ensino, Pesquisa e Extensão, Blumenau, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 9. | | PEDRI, Z. C.; LOZANO, L. M. S.; HERMANN, K. L.; HELM, C. V.; PERALTA, R. M.; TAVARES, L. B. B. Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii. Brazilian Journal of Biology, São Carlos, v. 75, n. 4, p. 940-947, Nov. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| 10. | | HERMANN, K, L.; COSTA, T. M.; HELM, C. V.; MARCONATTO, L.; BORGES, L. G. dos A.; VEGINI, A. A.; GIONGO, A.; TAVARES, L. B. B. Discoloration of rhodamine b dye by white-rot fungi in solid bleached sulfate paperboard coated with polyethylene terephthalate: scale-up into non-sterile packed-bed bioreactor. Journal of Environmental Chemical Engineering, v. 8, n. 3, 103685, June 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| 11. | | PEDRI, Z. C.; TAVARES, L. B. B.; LOZANO, L. M. dos S.; HERMANN, K. L.; VALLE, R. de C. S. C.; HELM, C. V.; MAGALHAES, W. L. E. Efeito de fatores de processo na atividade holocelulolítica e no crescimento micelial de Lentinula edodes. In: MOSTRA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 7., 2013, Blumenau. Anais. Blumenau: FURB, 2013. Resumo. Disponível online. MIPE.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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