Data/hora: 21/09/2024 06:13:33

Biblioteca(s): 	Embrapa Florestas.	
Data corrente: 	12/11/2020	
Data da última atualização: 	23/11/2020	
Autoria: 	GRUNENNVALDT, R. L.; DEGENHARDT, J.; BROOKS, P.; TOMASI, J. de C.; HANSEL, F. A.; TRAN, T.; GOMES, E. N.; DESCHAMPS, C.	
Afiliação: 	RENATA LÚCIA GRUNENNVALDT, UFPR; JULIANA DEGENHARDT, CNPF; PETER BROOKS, University of The Sunshine Coast; JÉSSICA DE CÁSSIA TOMASI, UFPR; FABRICIO AUGUSTO HANSEL, CNPF; TRONG TRAN, University of The Sunshine Coast; ERIK N. GOMES, The State University of New Jersey; CÍCERO DESCHAMPS, UFPR.	
Título: 	Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype infl uence, medium optimization and compounds identifi cation.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Anais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p.	
DOI: 	https://doi.org/10.1590/0001-3765202020181251	
Idioma: 	Inglês	
Conteúdo: 	Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.	
Palavras-Chave: 	Acúmulo de biomassa; Atividade antioxidante; Biomass accumulation; Erva mate; Plant growth regulators.	
Thesagro: 	Ilex Paraguariensis; Regulador de Crescimento; Sacarose.	
Thesaurus Nal: 	Antioxidant activity; Sucrose; Yerba mate.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/217736/1/Juliana-Grunennvaldt-et-al-2020.pdf	
Registro original: 	Embrapa Florestas (CNPF)	
Exemplares: 	CNPF - (57481-1) - UPC - AP - DD -  -  -  - DIS