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42. | | LUZ, N. B. da; OLIVEIRA, Y. M. M. de; ROSOT, M. A. D.; GARRASTAZU, M. C.; FRANCISCON, L. Classificação de imagens RapidEye baseada em objetos e análise da ecologia de paisagens como suporte ao componente geoespacial do Inventário Florestal Nacional. In: SIMPÓSIO NACIONAL DE INVENTÁRIO FLORESTAL, 2., 2013, Curitiba. Anais. Brasília, DF: Serviço Florestal Brasileiro, 2013. p. 139. Resumo. Biblioteca(s): Embrapa Florestas. |
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43. | | ANDRADE, G. de C.; BOGNOLA, I. A.; BELLOTE, A. F. J.; FRANCISCON, L.; WATERLOO, M. J.; BRUIJINZEEL, L. A. Site evaluation and productivity of a 3-year old stand of Eucalyptus urograndis in São Paulo, Brazil. Pesquisa Florestal Brasileira, Colombo, v. 31, n. 68, p. 331-346, out./dez. 2011. Biblioteca(s): Embrapa Florestas. |
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46. | | BOGNOLA, I. A.; CLASEN, L. A.; FRANCISCON, L.; GAVA, J. L.; DEDECEK, R. A.; SILVA, F. M. da. Aplicação de silicatos de cálcio e de potássio e o crescimento de mudas de Eucalyptus grandis. Pesquisa Florestal Brasileira, Colombo, v. 31, n. 66, p. 83-92, abr./jun. 2011. Biblioteca(s): Embrapa Florestas. |
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48. | | OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; BETTENCOURT, G. M. de F.; AMANO, E.; FRANCISCON, L.; QUOIRIN, M. Micropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone. Journal of Forestry Research, v. 28, n. 1, p. 29-39, Jan. 2017. Biblioteca(s): Embrapa Florestas. |
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50. | | COSTA JUNIOR, J. C. da; SANTOS, A. F. dos; FRANCISCON, L.; SILVA, C. N. da; TESSMANN, D. J. Qualidade sanitária e fisiológica, métodos de detecção de Fusarium spp. e tratamento de sementes de pupunheira. Ciência Florestal, Santa Maria, v. 26, n. 4, p. 1119-1131, out./dez. 2016. Biblioteca(s): Embrapa Florestas. |
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52. | | LUZ, N. B. da; OLIVEIRA, Y. M. M. de; ROSOT, M. A. D.; GARRASTAZU, M. C.; FRANCISCON, L.; MESQUITA JÚNIOR, H. N. de; FREITAS, J. V. de. Classificação híbrida de imagens Landsat-8 e RapidEye para o mapeamento do uso e cobertura da terra nas Unidades Amostrais de Paisagem do Inventário Florestal Nacional do Brasil. In: SIMPÓSIO BRASILEIRO DE SENSORIAMENTO REMOTO, 17., 2015, João Pessoa. Anais... São José dos Campos: INPE, 2015. p. 7222-7230. Disponível online. Biblioteca(s): Embrapa Florestas. |
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53. | | LUZ, N. B. da; OLIVEIRA, Y. M. M. de; ROSOT, M. A. D.; GARRASTAZU, M. C.; MATTOS, P. P. de; FRANCISCON, L.; FREITAS, J. V. de. Classificação de imagens orientada a objetos como suporte ao componente geoespacial do Inventário Florestal Nacional Brasileiro. In: SIMPÓSIO NACIONAL DE INVENTÁRIO FLORESTAL, 3., 2014, Manaus. Anais... Brasília, DF: Serviço Florestal Brasileiro, 2014. p. 126. Disponível online. Resumo. Biblioteca(s): Embrapa Florestas. |
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54. | | PARRON, L. M.; GARCIA, J. R.; RACHWAL, M. F. G.; FRANCHINI, J. C.; FRANCISCON, L.; PORFIRIO-DA-SILVA, V.; BROWN, G. G. Avaliação de serviços ambientais no âmbito do projeto ServiAmbi. In: PARRON, L. M.; GARCIA, J. R.; OLIVEIRA, E. B. de; BROWN, G. G.; PRADO, R. B. (Ed.). Serviços ambientais em sistemas agrícolas e florestais do Bioma Mata Atlântica. Brasília, DF : Embrapa, 2015. p. 36-46. Capítulo 2. Biblioteca(s): Embrapa Florestas; Embrapa Soja. |
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55. | | HOLLER, W. A.; ROSOT, M. A. D.; GARRASTAZU, M. C.; FIGUEIRA, I. F. R.; LUZ, N. B. da; MARAN, J. C.; FRANCISCON, L.; OLIVEIRA, Y. M. M. de. Dinâmica de uso e cobertura da terra e análise de tendência de mudanças para o município de Caçador, SC. Ciência e Natura, Santa Maria v. 40, e63, 2018. 22 p. Biblioteca(s): Embrapa Florestas. |
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56. | | MORITZ, A.; DEGENHARDT, J.; DUTRA, L. F.; HANSEL, F. A.; LIMA, B. H. de; FRANCESCHI, C. do R. B.; FRANCISCON, L. Efeito da sacarose sobre a germinação in vitro e crescimento inicial de embriões de imbuia. CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 17.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 4., 2009, Aracaju. Ciência, inovação e sustentabilidade. Aracaju: Embrapa Tabuleiros Costeiros; Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. Resumo. Biblioteca(s): Embrapa Florestas. |
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57. | | BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Florestas. |
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58. | | LUZ, N. B. da; GARRASTAZU, M. C.; ROSOT, M. A. D.; MARAN, J. C.; OLIVEIRA, Y. M. M. de; FRANCISCON, L.; CARDOSO, D. J.; FREITAS, J. V. de. Brazilian National Forest Inventory: a landscape scale approach to monitoring and assessing forested landscapes. Pesquisa Florestal Brasileira, Colombo, v. 38, e201701493, 2018. 14 p. Artigo de revisão. Biblioteca(s): Embrapa Florestas. |
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59. | | OLIVEIRA, Y. M. M. de; ROSOT, M. A. D.; GARRASTAZU, M. C.; LUZ, N.; MATTOS, P. P. de; FRANCISCON, L.; FREITAS, J.; PIOTTO, D.; GOMIDE, G. Brazilian national forest inventory: remote sensing and landscape analysis approaches for nationwide forest monitoring and assessment. The International Forestry Review, v. 16, n. 5, p. 206, 2014. Edição dos abstracts do 24º IUFRO World Congress, 2014, Salt Lake City. Sustaining forests, sustaining people: the role of research. Biblioteca(s): Embrapa Florestas. |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
31/03/2017 |
Data da última atualização: |
13/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; BETTENCOURT, G. M. de F.; AMANO, E.; FRANCISCON, L.; QUOIRIN, M. |
Afiliação: |
CASSIANA DE OLIVEIRA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; GISELA MANUELA DE FRANÇA BETTENCOURT; ERIKA AMANO, UFPR; LUZIANE FRANCISCON, CNPF; MARGUERITE QUOIRIN, UFPR. |
Título: |
Micropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 28, n. 1, p. 29-39, Jan. 2017. |
DOI: |
10.1007/s11676-016-0282-6 |
Idioma: |
Inglês |
Conteúdo: |
Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. MenosGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, a... Mostrar Tudo |
Palavras-Chave: |
Callogenesis; Eucalyptus urograndis; WPM culture medium. |
Thesagro: |
Micropropagação; Organogênese; Propagação vegetativa. |
Thesaurus NAL: |
Callus formation; Micropropagation; Organogenesis; Plant propagation. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02512naa a2200313 a 4500 001 2067974 005 2017-12-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-016-0282-6$2DOI 100 1 $aOLIVEIRA, C. de 245 $aMicropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone.$h[electronic resource] 260 $c2017 520 $aGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. 650 $aCallus formation 650 $aMicropropagation 650 $aOrganogenesis 650 $aPlant propagation 650 $aMicropropagação 650 $aOrganogênese 650 $aPropagação vegetativa 653 $aCallogenesis 653 $aEucalyptus urograndis 653 $aWPM culture medium 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aBETTENCOURT, G. M. de F. 700 1 $aAMANO, E. 700 1 $aFRANCISCON, L. 700 1 $aQUOIRIN, M. 773 $tJournal of Forestry Research$gv. 28, n. 1, p. 29-39, Jan. 2017.
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