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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
07/12/2022 |
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Data da última atualização: |
22/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B. de; MOLINA, J. C. J.; SILVA, R. S. da; RAMOS, A. F.; PURDY, P. H.; AZEVEDO, H. C. |
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Afiliação: |
MATHEUS BATISTA DE OLIVEIRA; JULIO CONSTANTINO JERI MOLINA; REBECA SANTOS DA SILVA; ALEXANDRE FLORIANI RAMOS, Cenargen; PHILLIP HAMILTON PURDY; HYMERSON COSTA AZEVEDO, CPATC. |
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Título: |
Effects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
CryoLetters, v. 43, n. 6, p. 357?367, 2022. |
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DOI: |
https://doi.org/10.54680/fr22610110812 |
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Idioma: |
Inglês |
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Conteúdo: |
Non-surgical artificial insemination techniques for sheep may benefit from larger numbers of sperm in the insemination dose because the ewe?s cervix is convoluted and often cannot be traversed with an insemination gun resulting in deposition of the sperm at the os cervix. OBJECTIVE: To compare a range of sperm concentrations when cryopreserving semen from Santa Inês rams and determine the effects of this on post-thaw quality. MATERIALS AND METHODS: One ejaculate from each ram (n = 10) was diluted to four sperm concentrations to obtain the following groups: G?400, G?800, G?1200, and G?1600 x 106 sperm/mL. The semen samples were packaged in 0.25 mL straws,cooled to 5 ºC, cryopreserved in liquid nitrogen vapor, thawed in a water bath (40 ºC/20 s), and were analyzed for computerized kinetics, capacitation and acrosome integrity, and plasma membrane integrity of sperm. RESULTS: The G?400 treatment resulted in samples with the highest linearity and progressive motion (P < 0.05) and had significantly greater plasma membrane integrity, and lower capacitation and acrosome reaction rates compared to G-1600 (P < 0.05). Overall, use of the G?400 treatment resulted in better kinetics, less plasma membrane damage and less early capacitation. However, despite reducing the ejaculate yield and increasing the costs of the semen freezing process, the G?800 and G?1200 treatments make a greater absolute number of sperm with good kinetics, plasma membrane integrity and capacitation status available. CONCLUSION: Ram sperm concentration impacts cryopreservation, and higher concentrations may be advantageous if a single artificial insemination protocol is desirable. MenosNon-surgical artificial insemination techniques for sheep may benefit from larger numbers of sperm in the insemination dose because the ewe?s cervix is convoluted and often cannot be traversed with an insemination gun resulting in deposition of the sperm at the os cervix. OBJECTIVE: To compare a range of sperm concentrations when cryopreserving semen from Santa Inês rams and determine the effects of this on post-thaw quality. MATERIALS AND METHODS: One ejaculate from each ram (n = 10) was diluted to four sperm concentrations to obtain the following groups: G?400, G?800, G?1200, and G?1600 x 106 sperm/mL. The semen samples were packaged in 0.25 mL straws,cooled to 5 ºC, cryopreserved in liquid nitrogen vapor, thawed in a water bath (40 ºC/20 s), and were analyzed for computerized kinetics, capacitation and acrosome integrity, and plasma membrane integrity of sperm. RESULTS: The G?400 treatment resulted in samples with the highest linearity and progressive motion (P < 0.05) and had significantly greater plasma membrane integrity, and lower capacitation and acrosome reaction rates compared to G-1600 (P < 0.05). Overall, use of the G?400 treatment resulted in better kinetics, less plasma membrane damage and less early capacitation. However, despite reducing the ejaculate yield and increasing the costs of the semen freezing process, the G?800 and G?1200 treatments make a greater absolute number of sperm with good kinetics, plasma membrane integrity and capacitation status availab... Mostrar Tudo |
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Palavras-Chave: |
Ovino Santa Iês. |
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Thesagro: |
Carneiro; Esperma; Inseminação; Inseminação Artificial; Ovelha; Ovino. |
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Thesaurus Nal: |
Rams; Sperm concentration. |
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Categoria do assunto: |
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Marc: |
LEADER 02544naa a2200301 a 4500
001 2150268
005 2022-12-22
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.54680/fr22610110812$2DOI
100 1 $aOLIVEIRA, M. B. de
245 $aEffects of cell concentration during cryopreservation on the post-thaw quality of Santa Inês ram sperm.$h[electronic resource]
260 $c2022
520 $aNon-surgical artificial insemination techniques for sheep may benefit from larger numbers of sperm in the insemination dose because the ewe?s cervix is convoluted and often cannot be traversed with an insemination gun resulting in deposition of the sperm at the os cervix. OBJECTIVE: To compare a range of sperm concentrations when cryopreserving semen from Santa Inês rams and determine the effects of this on post-thaw quality. MATERIALS AND METHODS: One ejaculate from each ram (n = 10) was diluted to four sperm concentrations to obtain the following groups: G?400, G?800, G?1200, and G?1600 x 106 sperm/mL. The semen samples were packaged in 0.25 mL straws,cooled to 5 ºC, cryopreserved in liquid nitrogen vapor, thawed in a water bath (40 ºC/20 s), and were analyzed for computerized kinetics, capacitation and acrosome integrity, and plasma membrane integrity of sperm. RESULTS: The G?400 treatment resulted in samples with the highest linearity and progressive motion (P < 0.05) and had significantly greater plasma membrane integrity, and lower capacitation and acrosome reaction rates compared to G-1600 (P < 0.05). Overall, use of the G?400 treatment resulted in better kinetics, less plasma membrane damage and less early capacitation. However, despite reducing the ejaculate yield and increasing the costs of the semen freezing process, the G?800 and G?1200 treatments make a greater absolute number of sperm with good kinetics, plasma membrane integrity and capacitation status available. CONCLUSION: Ram sperm concentration impacts cryopreservation, and higher concentrations may be advantageous if a single artificial insemination protocol is desirable.
650 $aRams
650 $aSperm concentration
650 $aCarneiro
650 $aEsperma
650 $aInseminação
650 $aInseminação Artificial
650 $aOvelha
650 $aOvino
653 $aOvino Santa Iês
700 1 $aMOLINA, J. C. J.
700 1 $aSILVA, R. S. da
700 1 $aRAMOS, A. F.
700 1 $aPURDY, P. H.
700 1 $aAZEVEDO, H. C.
773 $tCryoLetters$gv. 43, n. 6, p. 357?367, 2022.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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