Data/hora: 17/06/2024 01:11:54

Biblioteca(s): | Embrapa Milho e Sorgo; Embrapa Trigo.
Data corrente: | 06/04/2023
Data da última atualização: | 01/03/2024
Autoria: | RIBEIRO, M. R.; SIMEONE, M. L. F.; TRINDADE, R. dos S.; DIAS, L. A. dos S.; GUIMARAES, L. J. M.; TIBOLA, C. S.; AZEVEDO, T. C. de.
Afiliação: | MARIANA RODRIGUES RIBEIRO, Universidade Federal de Viçosa; MARIA LUCIA FERREIRA SIMEONE, CNPMS; ROBERTO DOS SANTOS TRINDADE, CNPMS; LUIZ ANTONIO DOS SANTOS DIAS, Universidade Federal de Viçosa; LAURO JOSE MOREIRA GUIMARAES, CNPMS; CASIANE SALETE TIBOLA, CNPT; TACILA CRISTINA DE AZEVEDO, Universidade Federal de São João del-Rei.
Título: | Near infrared spectroscopy (NIR) and chemometrics methods to identification of haploids in maize.
Ano de publicação: | 2023
Fonte/Imprenta: | Microchemical Journal, v. 190, 108604, 2023.
DOI: | https://doi.org/10.1016/j.microc.2023.108604
Idioma: | Inglês
Conteúdo: | Haploids in maize are selected through visual analysis, whether by use of expression of the R1-navajo marker in seeds, or by the phenotype of haploid plants in the field. These methods of identification of haploid plants demand space, resources, and time. Near infrared spectroscopy (NIR), together with multivariate calibration methods, is an alternative for identification of haploid seeds and plants in a quick and accurate way. The aim of the present study was to use NIR to classify haploid and diploid seeds and differentiate haploid, doubled haploid, and diploid plants originating from different genotypes used as donor. In the first experiment, haploid and diploid maize seeds were analyzed from four genotypes used as parents. In another experiment, diploid, haploid, and doubled haploid plants from 34 source populations were evaluated and differentiated. Spectral data were collected from all the seed and leaf samples using the portable MicroNIR device. Then the data were preprocessed by principal component analysis and by partial least squares discriminant analysis (PLS-DA). The use of the portable MicroNIR, together with the PLS-DA method, enabled classification of haploid and diploid seeds and haploid, doubled haploid, and diploid plants with 100% accuracy, constituting a fast, simple, nondestructive, and reliable methodology for identification of haploids in maize, and it is more effective than the visual analysis.
Palavras-Chave: | Análise multivariada; Duplo haploide; Multivariate analisys; Spectroscopic techniques; Técnica espectroscópica.
Thesagro: | Milho; Zea Mays.
Thesaurus Nal: | Doubled haploids.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
Registro original: | Embrapa Milho e Sorgo (CNPMS)

Exemplares:

CNPMS - (30067-1) - UPC - AP - DD -  -  -  - DIS
CNPT - (45513-1) - UPC - AP - DD -  -  -  - DIS