Data/hora: 09/06/2024 08:25:53

Biblioteca(s): | Embrapa Agricultura Digital; Embrapa Florestas.
Data corrente: | 13/11/2019
Data da última atualização: | 13/03/2020
Autoria: | BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J.
Afiliação: | Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF.
Título: | Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.
Ano de publicação: | 2020
Fonte/Imprenta: | Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020.
DOI: | 10.1007/s11676-018-0777-4
Idioma: | Inglês
Conteúdo: | Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated.
Palavras-Chave: | Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética.
Thesagro: | Eucalipto; Eucalyptus Urophylla.
Thesaurus Nal: | Genetic transformation; In vitro regeneration; Somaclonal variation.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Florestas (CNPF)

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