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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/05/2022 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. |
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Afiliação: |
THIAGO F. AMARAL, University of Florida; JOAO GABRIEL VIANA DE GRAZIA, FIVX Apoyar Biotech Ltda; LUANY ALVES GALVAO MARTINHAO, FIVX Apoyar Biotech Ltda; FELIPE DE COL, FIVX Apoyar Biotech Ltda; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen; PETER J. HANSEN, University of Florida. |
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Título: |
Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, 7503, 2022. |
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DOI: |
https://doi.org/10.1038/s41598-022-11447-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Procedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. MenosProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro w... Mostrar Tudo |
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Palavras-Chave: |
Fertilização; Produção in vitro. |
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Thesagro: |
Bovino; Embrião; Reprodução Animal. |
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Thesaurus Nal: |
Animal reproduction; Embryo (animal); In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142749/1/Actions-of-CSF2.pdf
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Marc: |
LEADER 02687naa a2200301 a 4500
001 2142749
005 2022-05-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-11447-7$2DOI
100 1 $aAMARAL, T. F.
245 $aActions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.$h[electronic resource]
260 $c2022
520 $aProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer.
650 $aAnimal reproduction
650 $aEmbryo (animal)
650 $aIn vitro fertilization
650 $aBovino
650 $aEmbrião
650 $aReprodução Animal
653 $aFertilização
653 $aProdução in vitro
700 1 $aGRAZIA, J. G. V. de
700 1 $aMARTINHAO, L A. G.
700 1 $aDE COL, F.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aVIANA, J. H. M.
700 1 $aHANSEN, P. J.
773 $tScientific Reports$gv. 12, 7503, 2022.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja; Embrapa Unidades Centrais. |
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Data corrente: |
06/02/2024 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
BROWN, G. G.; DEMETRIO, W.; GABRIAC, Q.; PASINI, A.; KORASAKI, V.; OLIVEIRA, L.; FRANCHINI, J. C.; TORRES, E.; GALERANI, P. R.; GAZZIERO, D. L. P.; BENITO, N. P.; NUNES, D. H.; SANTOS, A.; FERREIRA, T.; NADOLNY, H. S.; BARTZ, M.; MASCHIO, W.; DUDAS, R. T.; ZAGATTO, M.; NIVA, C. C.; CLASEN, L.; SAUTTER, K.; FROUFE, L. C. M.; SEOANE, C. E. S.; MORAES, A. de; JAMES, S.; ALBERTON, O.; JÚNIOR, O. B.; SARAIVA, O. F.; GARCIA, A.; OLIVEIRA, E.; CÉSAR, R.; CORREA-FERREIRA, B. S.; BRUZ, L. S. M.; SILVA, E. da; CARDOSO, G. B. X.; LAVELLE, P.; VELÁSQUEZ, E.; CREMONESI, M.; PARRON, L. M.; BAGGIO, A. J.; NEVES, E. J. M.; HUNGRIA, M.; CAMPOS, T. A.; SILVA, V. L. da; REISSMANN, C. B.; CONRADO, A. C.; BOUILLET, J. D.; GONÇALVES, J. L. M.; BRANDANI, C. B.; VIANI, R. A. G.; PAULA, R. R.; LACLAU, J.; PEÑA-VENEGAS, C. P.; PERES, C.; DECAËNS, T.; PEY, B.; EISENHAUER, N.; COOPER, M.; MATHIEU, J. |
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Afiliação: |
GEORGE GARDNER BROWN, CNPF; WILIAN DEMETRIO, UNIVERSIDADE DE SÃO PAULO; QUENTIN GABRIAC; AMARILDO PASINI, UNIVERSIDADE ESTADUAL DE LONDRINA; VANESCA KORASAKI, UNIVERSIDADE DO ESTADO DE MINAS GERAIS; LENITA OLIVEIRA; JULIO CEZAR FRANCHINI DOS SANTOS, CNPSO; ELENO TORRES; PAULO ROBERTO GALERANI, ARIN; DIONISIO LUIZ PISA GAZZIERO, CNPSO; NORTON POLO BENITO, Cenargen; DAIANE H. NUNES, INSTITUTO FEDERAL CATARINENSE; ALESSANDRA SANTOS, UNIVERSIDADE FEDERAL DO PARANÁ; TALITA FERREIRA, UNIVERSIDADE FEDERAL DO PARANÁ; HERLON S. NADOLNY, UNIVERSIDADE FEDERAL DO PARANÁ; MARIE BARTZ, CARE-BIO; WAGNER MASCHIO; RAFAELA T. DUDAS, UNIVERSIDADE FEDERAL DO PARANÁ; MAURICIO ZAGATTO, DUNGTECH BIOFERTILIZANTES; CINTIA CARLA NIVA, CNPSA; LINA CLASEN, UNIVERSITY OF COPENHAGEN; KLAUS SAUTTER; LUIS CLAUDIO MARANHAO FROUFE, CNPF; CARLOS EDUARDO SICOLI SEOANE, CNPF; ANÍBAL DE MORAES, UNIVERSIDADE FEDERAL DO PARANÁ; SAMUEL JAMES, MAHARISCHI UNIVERSITY; ODAIR ALBERTON, UNIVERSIDADE PARANAENSE; OSVALDINO BRANDÃO JÚNIOR, FATEC; ODILON FERREIRA SARAIVA, CNPSO; ANTONIO GARCIA, CNPSO; ELMA OLIVEIRA, UNIVERSIDADE FEDERAL DO PARANÁ; RAUL CÉSAR, UNIVERSIDADE FEDERAL DO PARANÁ; BEATRIZ SPALDING CORREA FERREIRA, CNPSO; LILIANNE S. M. BRUZ, UNIVERSIDADE FEDERAL DO PARANÁ; ELODIE DA SILVA; GILHERME B. X. CARDOSO, UNIVERSIDADE FEDERAL DO PARANÁ; PATRICK LAVELLE, UNIVERSITÉ PIERRE ET MARIE CURIE; ELENA VELÁSQUEZ, UNIVERSIDADE NACIONAL DE COLOMBIA; MARCUS CREMONESI, UNIVERSIDADE FEDERAL DO PARANÁ; LUCILIA MARIA PARRON VARGAS, CNPF; AMILTON JOAO BAGGIO, CNPF; EDINELSON JOSE MACIEL NEVES, CNPF; MARIANGELA HUNGRIA DA CUNHA, CNPSO; THIAGO A. CAMPOS, UNIVERSIDADE ESTADUAL DE LONDRINA; VAGNER L. DA SILVA, UNIVERSIDAD DE LA REPÚBLICA; CARLOS B. REISSMANN, UNIVERSIDADE FEDERAL DO PARANÁ; ANA C. CONRADO, UNIVERSIDADE FEDERAL DO PARANÁ; JEAN-PIERRE D. BOUILLET, CIRAD; JOSÉ L. M. GONÇALVES, UNIVERSIDADE DE SÃO PAULO; CAROLINA B. BRANDANI, TEXAS A&M AGRILIFE; RICARDO A. G. VIANI, UNIVERSIDADE FEDERAL DE SÃO CARLOS; RANIERI R. PAULA, UNIVERSITÉ DU QUÉBEC; JEAN-PAUL LACLAU, CIRAD; CLARA P. PEÑA-VENEGAS, SINCHI; CARLOS PERES, UNIVERSITY OF EAST ANGLIA; THIBAUD DECAËNS, UNIV MONTPELLIER; BENJAMIN PEY, UNIVERSITÉ DE TOULOUSE; NICO EISENHAUER, GERMAN CENTRE FOR INTEGRATIVE BIODIVERSITY RESEARCH; MIGUEL COOPER, UNIVERSIDADE DE SÃO PAULO; JÉRÔME MATHIEU, SORBONNE UNIVERSITÉ. |
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Título: |
Soil macrofauna communities in Brazilian land-use systems. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Biodiversity Data Journal, v. 12, e115000, 2024. |
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DOI: |
https://doi.org/10.3897/BDJ.12.e115000 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Soil animal communities include more than 40 higher-order taxa, representing over 23% of all described species. These animals have a wide range of feeding sources and contribute to several important soil functions and ecosystem services. Although many studies have assessed macroinvertebrate communities in Brazil, few of them have been published in journals and even fewer have made the data openly available for consultation and further use. As part of ongoing efforts to synthesise the global soil macrofauna communities and to increase the amount of openly-accessible data in GBIF and other repositories related to soil biodiversity, the present paper provides links to 29 soil macroinvertebrate datasets covering 42 soil fauna taxa, collected in various land-use systems in Brazil. A total of 83,085 georeferenced occurrences of these taxa are presented, based on quantitative estimates performed using a standardised sampling method commonly adopted worldwide to collect soil macrofauna populations, i.e. the TSBF (Tropical Soil Biology and Fertility Programme) protocol. This consists of digging soil monoliths of 25 x 25 cm area, with handsorting of the macroinvertebrates visible to the naked eye from the surface litter and from within the soil, typically in the upper 0-20 cm layer (but sometimes shallower, i.e. top 0-10 cm or deeper to 0-40 cm, depending on the site). The land-use systems included anthropogenic sites managed with agricultural systems (e.g. pastures, annual and perennial crops, agroforestry), as well as planted forests and native vegetation located mostly in the southern Brazilian State of Paraná (96 sites), with a few additional sites in the neighbouring states of São Paulo (21 sites) and Santa Catarina (five sites). Important metadata on soil properties, particularly soil chemical parameters (mainly pH, C, P, Ca, K, Mg, Al contents, exchangeable acidity, Cation Exchange Capacity, Base Saturation and, infrequently, total N), particle size distribution (mainly % sand, silt and clay) and, infrequently, soil moisture and bulk density, as well as on human management practices (land use and vegetation cover) are provided. These data will be particularly useful for those interested in estimating land-use change impacts on soil biodiversity and its implications for below-ground foodwebs, ecosystem functioning and ecosystem service delivery. MenosSoil animal communities include more than 40 higher-order taxa, representing over 23% of all described species. These animals have a wide range of feeding sources and contribute to several important soil functions and ecosystem services. Although many studies have assessed macroinvertebrate communities in Brazil, few of them have been published in journals and even fewer have made the data openly available for consultation and further use. As part of ongoing efforts to synthesise the global soil macrofauna communities and to increase the amount of openly-accessible data in GBIF and other repositories related to soil biodiversity, the present paper provides links to 29 soil macroinvertebrate datasets covering 42 soil fauna taxa, collected in various land-use systems in Brazil. A total of 83,085 georeferenced occurrences of these taxa are presented, based on quantitative estimates performed using a standardised sampling method commonly adopted worldwide to collect soil macrofauna populations, i.e. the TSBF (Tropical Soil Biology and Fertility Programme) protocol. This consists of digging soil monoliths of 25 x 25 cm area, with handsorting of the macroinvertebrates visible to the naked eye from the surface litter and from within the soil, typically in the upper 0-20 cm layer (but sometimes shallower, i.e. top 0-10 cm or deeper to 0-40 cm, depending on the site). The land-use systems included anthropogenic sites managed with agricultural systems (e.g. pastures, annual and perenn... Mostrar Tudo |
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Palavras-Chave: |
Invertebrados; Macrofauna do solo; Serviços ecossistêmicos; Soil macrofauna. |
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Thesagro: |
Biodiversidade; Ecossistema; Solo; Taxonomia Animal; Uso da Terra. |
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Thesaurus NAL: |
Biodiversity; ecology; Ecosystem services; Land use. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161664/1/BiodiversDataJ-2024-Brown.pdf
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Marc: |
LEADER 04955naa a2200997 a 4500
001 2161664
005 2024-02-06
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3897/BDJ.12.e115000$2DOI
100 1 $aBROWN, G. G.
245 $aSoil macrofauna communities in Brazilian land-use systems.$h[electronic resource]
260 $c2024
520 $aSoil animal communities include more than 40 higher-order taxa, representing over 23% of all described species. These animals have a wide range of feeding sources and contribute to several important soil functions and ecosystem services. Although many studies have assessed macroinvertebrate communities in Brazil, few of them have been published in journals and even fewer have made the data openly available for consultation and further use. As part of ongoing efforts to synthesise the global soil macrofauna communities and to increase the amount of openly-accessible data in GBIF and other repositories related to soil biodiversity, the present paper provides links to 29 soil macroinvertebrate datasets covering 42 soil fauna taxa, collected in various land-use systems in Brazil. A total of 83,085 georeferenced occurrences of these taxa are presented, based on quantitative estimates performed using a standardised sampling method commonly adopted worldwide to collect soil macrofauna populations, i.e. the TSBF (Tropical Soil Biology and Fertility Programme) protocol. This consists of digging soil monoliths of 25 x 25 cm area, with handsorting of the macroinvertebrates visible to the naked eye from the surface litter and from within the soil, typically in the upper 0-20 cm layer (but sometimes shallower, i.e. top 0-10 cm or deeper to 0-40 cm, depending on the site). The land-use systems included anthropogenic sites managed with agricultural systems (e.g. pastures, annual and perennial crops, agroforestry), as well as planted forests and native vegetation located mostly in the southern Brazilian State of Paraná (96 sites), with a few additional sites in the neighbouring states of São Paulo (21 sites) and Santa Catarina (five sites). Important metadata on soil properties, particularly soil chemical parameters (mainly pH, C, P, Ca, K, Mg, Al contents, exchangeable acidity, Cation Exchange Capacity, Base Saturation and, infrequently, total N), particle size distribution (mainly % sand, silt and clay) and, infrequently, soil moisture and bulk density, as well as on human management practices (land use and vegetation cover) are provided. These data will be particularly useful for those interested in estimating land-use change impacts on soil biodiversity and its implications for below-ground foodwebs, ecosystem functioning and ecosystem service delivery.
650 $aBiodiversity
650 $aecology
650 $aEcosystem services
650 $aLand use
650 $aBiodiversidade
650 $aEcossistema
650 $aSolo
650 $aTaxonomia Animal
650 $aUso da Terra
653 $aInvertebrados
653 $aMacrofauna do solo
653 $aServiços ecossistêmicos
653 $aSoil macrofauna
700 1 $aDEMETRIO, W.
700 1 $aGABRIAC, Q.
700 1 $aPASINI, A.
700 1 $aKORASAKI, V.
700 1 $aOLIVEIRA, L.
700 1 $aFRANCHINI, J. C.
700 1 $aTORRES, E.
700 1 $aGALERANI, P. R.
700 1 $aGAZZIERO, D. L. P.
700 1 $aBENITO, N. P.
700 1 $aNUNES, D. H.
700 1 $aSANTOS, A.
700 1 $aFERREIRA, T.
700 1 $aNADOLNY, H. S.
700 1 $aBARTZ, M.
700 1 $aMASCHIO, W.
700 1 $aDUDAS, R. T.
700 1 $aZAGATTO, M.
700 1 $aNIVA, C. C.
700 1 $aCLASEN, L.
700 1 $aSAUTTER, K.
700 1 $aFROUFE, L. C. M.
700 1 $aSEOANE, C. E. S.
700 1 $aMORAES, A. de
700 1 $aJAMES, S.
700 1 $aALBERTON, O.
700 1 $aJÚNIOR, O. B.
700 1 $aSARAIVA, O. F.
700 1 $aGARCIA, A.
700 1 $aOLIVEIRA, E.
700 1 $aCÉSAR, R.
700 1 $aCORREA-FERREIRA, B. S.
700 1 $aBRUZ, L. S. M.
700 1 $aSILVA, E. da
700 1 $aCARDOSO, G. B. X.
700 1 $aLAVELLE, P.
700 1 $aVELÁSQUEZ, E.
700 1 $aCREMONESI, M.
700 1 $aPARRON, L. M.
700 1 $aBAGGIO, A. J.
700 1 $aNEVES, E. J. M.
700 1 $aHUNGRIA, M.
700 1 $aCAMPOS, T. A.
700 1 $aSILVA, V. L. da
700 1 $aREISSMANN, C. B.
700 1 $aCONRADO, A. C.
700 1 $aBOUILLET, J. D.
700 1 $aGONÇALVES, J. L. M.
700 1 $aBRANDANI, C. B.
700 1 $aVIANI, R. A. G.
700 1 $aPAULA, R. R.
700 1 $aLACLAU, J.
700 1 $aPEÑA-VENEGAS, C. P.
700 1 $aPERES, C.
700 1 $aDECAËNS, T.
700 1 $aPEY, B.
700 1 $aEISENHAUER, N.
700 1 $aCOOPER, M.
700 1 $aMATHIEU, J.
773 $tBiodiversity Data Journal$gv. 12, e115000, 2024.
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