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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
23/05/2014 |
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Data da última atualização: |
23/05/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BALIEIRO, F. de C.; CHAER, G. M.; SANTOS, F. M.; DINIZ, A. R.; PICCOLO, M. de C. |
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Afiliação: |
FABIANO DE CARVALHO BALIEIRO, CNPS; GUILHERME MONTANDON CHAER, CNPAB; Felipe Martini Santos, CNPAB; Anderson Ribeiro Diniz, UFRRJ; Marisa de Cássia Piccolo, Centro de energia nuclear de agricultura. |
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Título: |
Mixed-species plantations of Acacia mangium and eucalyptus urograndis in Southeast Brazil: aboveground biomass, nutrition and soil fertility. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: ACACIA, 2014, Hue, Vietnan. Resumo.... Vienna, Austria: IUFRO, 2014. |
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Idioma: |
Português |
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Palavras-Chave: |
Eucalyptus urograndis; Southeast Brazil. |
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Thesaurus Nal: |
Acacia mangium. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102564/1/Impressao-de-fax-em-pagina-inteira.pdf
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Marc: |
LEADER 00650nam a2200181 a 4500
001 1986959
005 2014-05-23
008 2014 bl uuuu u00u1 u #d
100 1 $aBALIEIRO, F. de C.
245 $aMixed-species plantations of Acacia mangium and eucalyptus urograndis in Southeast Brazil$baboveground biomass, nutrition and soil fertility.$h[electronic resource]
260 $aIn: ACACIA, 2014, Hue, Vietnan. Resumo.... Vienna, Austria: IUFRO$c2014
650 $aAcacia mangium
653 $aEucalyptus urograndis
653 $aSoutheast Brazil
700 1 $aCHAER, G. M.
700 1 $aSANTOS, F. M.
700 1 $aDINIZ, A. R.
700 1 $aPICCOLO, M. de C.
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Registro original: |
Embrapa Solos (CNPS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/03/2016 |
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Data da última atualização: |
30/03/2016 |
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Autoria: |
CARVALHO, Y. K. P. de; ARGÔLO-NETO, N. M.; AMBRÓSIO, C. E.; OLIVEIRA, L. de J. de; ROCHA, A. R. da; SILVA, J. B. da; CARVALHO, M. A. M. de; ALVES, F. R. |
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Afiliação: |
YULLA K. P. DE CARVALHO, UFPI; NAPOLEÃO M. ARGÔLO-NETO, UFPI; CARLOS E. AMBRÓSIO, USP; LILIAN DE JESUS DE OLIVEIRA, USP; ANDRESSA R. DA ROCHA, UFPI; JANETE B. DA SILVA, UFPI; MARIA ACELINA M. DE CARVALHO, UFPI; FLÁVIO R. ALVES, UFPI. |
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Título: |
Isolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831). |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 590-598, jun. 2015 |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue. |
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Palavras-Chave: |
Biologia celular; Cultivo celular; Dasyprocta prymnolopha; Diferenciação celular; MSC da polpa dentária; MSC from dental pulp. |
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Thesaurus NAL: |
Cell biology; Cell culture; Cell differentiation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141856/1/Isolation-expansion.pdf
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Marc: |
LEADER 02395naa a2200313 a 4500
001 2042235
005 2016-03-30
008 2015 bl uuuu u00u1 u #d
100 1 $aCARVALHO, Y. K. P. de
245 $aIsolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831).
260 $c2015
520 $aThe study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue.
650 $aCell biology
650 $aCell culture
650 $aCell differentiation
653 $aBiologia celular
653 $aCultivo celular
653 $aDasyprocta prymnolopha
653 $aDiferenciação celular
653 $aMSC da polpa dentária
653 $aMSC from dental pulp
700 1 $aARGÔLO-NETO, N. M.
700 1 $aAMBRÓSIO, C. E.
700 1 $aOLIVEIRA, L. de J. de
700 1 $aROCHA, A. R. da
700 1 $aSILVA, J. B. da
700 1 $aCARVALHO, M. A. M. de
700 1 $aALVES, F. R.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 6, p. 590-598, jun. 2015
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