Data/hora: 24/06/2024 00:49:49

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 15/03/2018
Data da última atualização: | 16/01/2019
Autoria: | PINTO, P. H. N.; BALARO, M. F. A.; SOUZA-FABJAN, J. M. G.; RIBEIRO, L. dos S.; BRAGANÇA, G. M.; LEITE, C. R.; ARASHIRO, E. K. N.; SILVA, K. de M.; FONSECA, J. F. da; BRANDÃO, F. Z.
Afiliação: | Pedro Henrique Nicolau Pinto, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Mario Felipe Alvarez Balaro, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Joanna Maria Gonçalves Souza-Fabjan, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Lilian dos Santos Ribeiro, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Glaucia Mota Bragança, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Ceci Ribeiro Leite, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Eduardo Kenji Nunes Arashiro, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; KLEIBE DE MORAES SILVA, CNPC; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.
Título: | Anti-Müllerian hormone and antral follicle count are more effective for selecting ewes with good potential for in vivo embryo production than the presence of FecGE mutation or eCG pre-selection tests.
Ano de publicação: | 2018
Fonte/Imprenta: | Theriogenology, v. 113, p. 146-152, Feb. 2018.
DOI: | 10.1016/j.theriogenology.2018.02.018
Idioma: | Inglês
Conteúdo: | Abstract: This study aims to compare four different methods for selecting high responding sheep donors for in vivo embryo production. These methods include a pre-selection eCG test (eCG), antral follicle count (AFC), plasma anti-Müllerian hormone measurement (AMH) and genotyping for the presence of the FecGE mutation (a polymorphism in the GDF9 gene associated with increased ovulation rate). Santa Ines ewe lambs (n = 25) underwent superovulation (SOV) with 800 IU equine chorionic gonadotropin (eCG) and the corpus luteum (CL) count was recorded by laparoscopy after eight days. At the D0eCG, blood samples for AMH and genotyping analysis were collected. Twenty-one days after the end of the eCG test, the same animals underwent SOV with 200 mg of FSH, administered in six decreasing doses, and then naturally mated. Immediately before the beginning of the FSH protocol (D0FSH), and at the moment of the first FSH dose (D9FSH), the AFC was assessed. Plasma AMH was again determined at the D9FSH. After each screening process, animals were classified as having a high (HR), or low (LR), potential of response (using specific thresholds for each method). Then, the ewes' response to SOV and embryo yield for each screening method, classified as HR or LR, were compared. Animals classified as HR by AFC (HRAFC) and by AMH concentration (HRAMH) at the D9FSH, produced more viable embryos than those classified as LRAFC and LRAMH (HRAFC 6.2 ± 3.2 vs LRAFC 2.8 ± 3.0 and HRAMH 6.6 ± 3.6 vs LRAMH 3.0 ± 2.9). Pre-selection tests with eCG and different FecGE genotypes, either heterozygous (+/E) or wild type (+/+), were unable to discriminate HR or LR animals. A tendency (P = 0.06) to have lower plasma AMH was observed in heterozygous FecGE (+/E) ewes. In conclusion, both AFC and plasma AMH can be used to select donor ewes with a higher potential of response for in vivo embryo production.
Palavras-Chave: | Donor animals; Donor selection; MOET; Raça Santa Inês.
Thesagro: | Ovelha; Ovino; Reprodução animal; Superovulação.
Thesaurus Nal: | Reproduction; Sheep; Superovulation.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (36739-1) - UPC - AP - PP -  -  -  - DIS