Data/hora: 23/06/2024 13:13:42

Biblioteca(s): | Embrapa Milho e Sorgo.
Tipo da produção científica: | Artigo em Periódico Indexado
Data corrente: | 26/09/2000
Data da última atualização: | 08/06/2018
Autoria: | VALICENTE, F. H.; BARRETO, M. R.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; PAIVA, E.
Afiliação: | FERNANDO HERCOS VALICENTE, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS.
Título: | Identificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae).
Ano de publicação: | 2000
Fonte/Imprenta: | Anais da Sociedade Entomológica do Brasil, Jaboticabal, v. 29, n. 1, p. 147-153, 2000.
Idioma: | Português
Conteúdo: | Fall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains.
Palavras-Chave: | Cry1; Lagarta do cartucho.
Thesagro: | Controle Biológico; Spodoptera Frugiperda.
Thesaurus Nal: | biological control; Insecta.
Categoria do assunto: | O Insetos e Entomologia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/45185/1/Identificacao-atraves.pdf
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (12552-1) - UPC - AP - DD -  -  -  - DIS