|
|
Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
31/03/2009 |
Data da última atualização: |
08/03/2010 |
Tipo da produção científica: |
Documentos |
Autoria: |
BERGIER, I.; ISHII, I. I.; SALIS, S. M.; PELEGRIN, L. A.; RESENDE, E. K. de; TOMAS, W. M.; SOARES, M. T. S. |
Afiliação: |
Ivan Bergier, Embrapa Pantanal; Iria Iromi Ishii, UFMS; Suzana Maria Salis, Embrapa Pantanal; Luiz Alberto Pellegrin, Embrapa Pantanal; Emiko Kawakami de Resende, Embrapa Pantanal; Walfrido Moraes Tomás, Embrapa Pantanal; Márcia Toffani Simão Soares, Embrapa Pantanal. |
Título: |
Cenários de desenvolvimento sustentável no Pantanal em função de tendências hidroclimáticas. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Corumbá: Embrapa Pantanal, 2008. |
Páginas: |
21 p. |
Série: |
(Embrapa Pantanal. Documentos, 98). |
Idioma: |
Português |
Conteúdo: |
Nesta feita uma análise prospectiva do futuro hidrológico do Pantanal sob a perspectiva de dados pretéritos, da mudança do clima com base em relações entre o nível máximo do Rio Paraguai, interação oceano-atmosfera e precipitação na América do Sul. A cenarização hidroclimática, incluindo a componente humana, permite ainda identificar adaptações e oportunidades para as próximas décadas, também em função de mudanças e tendências da economia mundial. |
Palavras-Chave: |
Mudanças climáticas. |
Thesagro: |
Desenvolvimento Sustentável; Hidrologia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAP-2009-09/56873/1/DOC98.pdf
|
Marc: |
LEADER 01177nam a2200241 a 4500 001 1798808 005 2010-03-08 008 2008 bl uuuu u0uu1 u #d 100 1 $aBERGIER, I. 245 $aCenários de desenvolvimento sustentável no Pantanal em função de tendências hidroclimáticas. 260 $aCorumbá: Embrapa Pantanal$c2008 300 $a21 p. 490 $a(Embrapa Pantanal. Documentos, 98). 520 $aNesta feita uma análise prospectiva do futuro hidrológico do Pantanal sob a perspectiva de dados pretéritos, da mudança do clima com base em relações entre o nível máximo do Rio Paraguai, interação oceano-atmosfera e precipitação na América do Sul. A cenarização hidroclimática, incluindo a componente humana, permite ainda identificar adaptações e oportunidades para as próximas décadas, também em função de mudanças e tendências da economia mundial. 650 $aDesenvolvimento Sustentável 650 $aHidrologia 653 $aMudanças climáticas 700 1 $aISHII, I. I. 700 1 $aSALIS, S. M. 700 1 $aPELEGRIN, L. A. 700 1 $aRESENDE, E. K. de 700 1 $aTOMAS, W. M. 700 1 $aSOARES, M. T. S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pantanal (CPAP) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
14/02/2011 |
Data da última atualização: |
19/07/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
Thesaurus NAL: |
mitochondrial DNA. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
|
Marc: |
LEADER 03301naa a2200313 a 4500 001 1876985 005 2022-07-19 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.rbmo.2010.10.011$2DOI 100 1 $aCHIARATTI, M. R. 245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource] 260 $c2011 520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. 650 $amitochondrial DNA 653 $aEmbryo 653 $aEthidium bromide 653 $aOocyte 653 $aOoplasm 653 $aTransfer 700 1 $aFERREIRA, C. R. 700 1 $aPERECIN, F. 700 1 $aNICIURA, S. C. M. 700 1 $aSANGALLI, J. R. 700 1 $aMESQUITA, L. G. 700 1 $aBALIEIRO, J. C. de C. 700 1 $aSMITH, L. C. 700 1 $aGARCIA, J. M. 700 1 $aMEIRELLES, F. V. 773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|