Data/hora: 13/06/2024 08:04:47

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 30/03/2016
Data da última atualização: | 30/03/2016
Autoria: | CARVALHO, Y. K. P. de; ARGÔLO-NETO, N. M.; AMBRÓSIO, C. E.; OLIVEIRA, L. de J. de; ROCHA, A. R. da; SILVA, J. B. da; CARVALHO, M. A. M. de; ALVES, F. R.
Afiliação: | YULLA K. P. DE CARVALHO, UFPI; NAPOLEÃO M. ARGÔLO-NETO, UFPI; CARLOS E. AMBRÓSIO, USP; LILIAN DE JESUS DE OLIVEIRA, USP; ANDRESSA R. DA ROCHA, UFPI; JANETE B. DA SILVA, UFPI; MARIA ACELINA M. DE CARVALHO, UFPI; FLÁVIO R. ALVES, UFPI.
Título: | Isolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831).
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 590-598, jun. 2015
Idioma: | Inglês
Conteúdo: | The study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue.
Palavras-Chave: | Biologia celular; Cultivo celular; Dasyprocta prymnolopha; Diferenciação celular; MSC da polpa dentária; MSC from dental pulp.
Thesaurus Nal: | Cell biology; Cell culture; Cell differentiation.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/141856/1/Isolation-expansion.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59234-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS