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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
06/04/2004 |
Data da última atualização: |
27/07/2007 |
Autoria: |
TOLEDO, A. M.; HOFFMANN-CAMPO, C. B.; MIYAKUBO, S. H.; PIUBELLI, G. C. |
Título: |
Time of response and quantification of isoflavone in soybean genotypes after damage by Piezodorus guildinii. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
Páginas: |
p. 218. |
Série: |
(Embrapa Soja. Documentos, 228).
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Idioma: |
Inglês |
Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
Conteúdo: |
The induction of response to herbivory by production de novo or by augmentation of constitutive chemical compounds has been reported in various plants. Previous experiments carried out at the Embrapa Soybean Phytochemistry Laboratory, Londrina, PR, Brazil showed an increase in isoflavone content (induction) in some soybean genotypes after being damaged by Nezara viridula, as well as its negative effect on the insect feeding preference. Experiments were performed to evaluate the response time and the production of the isoflavones daidzin and genistin by several resistant genotypes (PI 229358, PI 171451, PI 274454, 'BRSMG 68', 'IAC 100'), as compared to a susceptible genotype ('Embrapa 4'). In the greenhouse, four P. guildinii (starved for 24h) were placed in cages (made from two Petri dishes bottoms) containing two soybean pods settled in a live plant of each tested genotype; insect-free cages were maintained as a control. After 18h of confining, the insects were removed from the cages and the pods were excised at 3, 7 and 15 days after herbivory (DAH) for seed extract preparation. An aliquot of the extract of each genotype was analyzed by HPLC to estimate the concentration of daidzin and genistin comparing with those obtained from pure compounds (standards). In general, the constitutive concentration of genistin (non-injured) was higher than daidzin in most of the tested genotypes. The maximum concentration of genistin in non-injured seeds was observed at 15 DAH, indicating an increase in this isoflavone concentration at the later stages of plant development. The injured and non-injured seeds of most genotypes produced the same amount of daidzin with exception of PI 171451 and 'BRSMG 68' at 3 and 7 DAH, respectively. At 15 DAH the concentration of genistin in the non-injured seeds was higher than in the injured seeds, with the exception of the genotype PI 274454. On the other hands, a gradual increase of daidzin concentration was observed over the time, mainly in injured seeds. At 3 DAH, the concentration of daidzin raised from 0.005, in non-injured to 0.169 mg/mL in injured seeds. However the most evident response was observed in the genotype 'BRSMG 68', at 7 and 15 DAH; daidzin concentration in injured seeds increased about three times (from 0,066 to 0,217 mg/mL and 0.087 to 0.229), compared with non-injured seeds. The results indicated that isoflavone concentration depends on the time, stage of plant development, genotype and may be induced by insect damage. MenosThe induction of response to herbivory by production de novo or by augmentation of constitutive chemical compounds has been reported in various plants. Previous experiments carried out at the Embrapa Soybean Phytochemistry Laboratory, Londrina, PR, Brazil showed an increase in isoflavone content (induction) in some soybean genotypes after being damaged by Nezara viridula, as well as its negative effect on the insect feeding preference. Experiments were performed to evaluate the response time and the production of the isoflavones daidzin and genistin by several resistant genotypes (PI 229358, PI 171451, PI 274454, 'BRSMG 68', 'IAC 100'), as compared to a susceptible genotype ('Embrapa 4'). In the greenhouse, four P. guildinii (starved for 24h) were placed in cages (made from two Petri dishes bottoms) containing two soybean pods settled in a live plant of each tested genotype; insect-free cages were maintained as a control. After 18h of confining, the insects were removed from the cages and the pods were excised at 3, 7 and 15 days after herbivory (DAH) for seed extract preparation. An aliquot of the extract of each genotype was analyzed by HPLC to estimate the concentration of daidzin and genistin comparing with those obtained from pure compounds (standards). In general, the constitutive concentration of genistin (non-injured) was higher than daidzin in most of the tested genotypes. The maximum concentration of genistin in non-injured seeds was observed at 15 DAH, indicating ... Mostrar Tudo |
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Marc: |
LEADER 03445naa a2200193 a 4500 001 1466804 005 2007-07-27 008 2004 bl uuuu u00u1 u #d 100 1 $aTOLEDO, A. M. 245 $aTime of response and quantification of isoflavone in soybean genotypes after damage by Piezodorus guildinii. 260 $c2004 300 $ap. 218. 490 $a(Embrapa Soja. Documentos, 228). 500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. 520 $aThe induction of response to herbivory by production de novo or by augmentation of constitutive chemical compounds has been reported in various plants. Previous experiments carried out at the Embrapa Soybean Phytochemistry Laboratory, Londrina, PR, Brazil showed an increase in isoflavone content (induction) in some soybean genotypes after being damaged by Nezara viridula, as well as its negative effect on the insect feeding preference. Experiments were performed to evaluate the response time and the production of the isoflavones daidzin and genistin by several resistant genotypes (PI 229358, PI 171451, PI 274454, 'BRSMG 68', 'IAC 100'), as compared to a susceptible genotype ('Embrapa 4'). In the greenhouse, four P. guildinii (starved for 24h) were placed in cages (made from two Petri dishes bottoms) containing two soybean pods settled in a live plant of each tested genotype; insect-free cages were maintained as a control. After 18h of confining, the insects were removed from the cages and the pods were excised at 3, 7 and 15 days after herbivory (DAH) for seed extract preparation. An aliquot of the extract of each genotype was analyzed by HPLC to estimate the concentration of daidzin and genistin comparing with those obtained from pure compounds (standards). In general, the constitutive concentration of genistin (non-injured) was higher than daidzin in most of the tested genotypes. The maximum concentration of genistin in non-injured seeds was observed at 15 DAH, indicating an increase in this isoflavone concentration at the later stages of plant development. The injured and non-injured seeds of most genotypes produced the same amount of daidzin with exception of PI 171451 and 'BRSMG 68' at 3 and 7 DAH, respectively. At 15 DAH the concentration of genistin in the non-injured seeds was higher than in the injured seeds, with the exception of the genotype PI 274454. On the other hands, a gradual increase of daidzin concentration was observed over the time, mainly in injured seeds. At 3 DAH, the concentration of daidzin raised from 0.005, in non-injured to 0.169 mg/mL in injured seeds. However the most evident response was observed in the genotype 'BRSMG 68', at 7 and 15 DAH; daidzin concentration in injured seeds increased about three times (from 0,066 to 0,217 mg/mL and 0.087 to 0.229), compared with non-injured seeds. The results indicated that isoflavone concentration depends on the time, stage of plant development, genotype and may be induced by insect damage. 700 1 $aHOFFMANN-CAMPO, C. B. 700 1 $aMIYAKUBO, S. H. 700 1 $aPIUBELLI, G. C. 773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
10/01/2018 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, D. F.; PEIXOTO, R. M.; ARAÚJO, J. F.; SILVA, A. F.; SANTOS, D. O.; ANDRIOLI, A. |
Afiliação: |
Davi Farias Silva, Graduação - UNINTA - Sobral, CE, Brasil; Renato Mesquita Peixoto, Pós-graduação -Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brasil; Juscilância Furtado Araújo, Pós-graduação - Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; Adriano Ferreira Silva, Graduação - UNINTA - Sobral, CE, Brasil; DIONES OLIVEIRA SANTOS, CNPC; ALICE ANDRIOLI, CNPC. |
Título: |
Avaliação das patologias espermáticas de caprinos Anglo-Nubianos portadores do vírus da artrite encefalite caprina diluídos em MEM e Tris-gema. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 6., 2017, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2017. p. 46-47. |
Idioma: |
Português |
Notas: |
[ALICE ANDRIOLI PINHEIRO]. |
Conteúdo: |
Os espermatozoides são células haploides altamente especializadas, cuja função, juntamente com o oócito, é propagar as informações genéticas de sua espécie. A avaliação da morfologia espermática é um importante indicador de fertilidade, sendo preconizado pelo Colégio Brasileiro de Reprodução Animal (CBRA, 2013) que as partidas de sêmen de caprinos que contenham mais de 20% de anormalidades não devem ser usadas em programas de inseminação artificial. O objetivo do trabalho foi conferir se o vírus da Artrite Encefalite Caprina (CAEV) interfere na patologia espermática e qual seria o melhor diluente a ser utilizado em futuros experimentos com acréscimo de antivirais ao diluente. O |
Palavras-Chave: |
CAEV; Caprine arthritis encephalit virus; Defesa antiviral; Diluente; Meio Essencial Mínimo; MEM; Morfologia espermática; Parâmetros espermáticos; Semen preservation; Tris gema; Vírus da Artrite Encefalite Caprina. |
Thesagro: |
Caprino; Conservação; Espermatozóide; Sêmen. |
Thesaurus NAL: |
Goats; Spermatozoa. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171943/1/CNPC-2017-Avaliacao-das-patologias.pdf
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Marc: |
LEADER 01973nam a2200385 a 4500 001 2084956 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aSILVA, D. F. 245 $aAvaliação das patologias espermáticas de caprinos Anglo-Nubianos portadores do vírus da artrite encefalite caprina diluídos em MEM e Tris-gema.$h[electronic resource] 260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 6., 2017, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2017. p. 46-47.$c2017 500 $a[ALICE ANDRIOLI PINHEIRO]. 520 $aOs espermatozoides são células haploides altamente especializadas, cuja função, juntamente com o oócito, é propagar as informações genéticas de sua espécie. A avaliação da morfologia espermática é um importante indicador de fertilidade, sendo preconizado pelo Colégio Brasileiro de Reprodução Animal (CBRA, 2013) que as partidas de sêmen de caprinos que contenham mais de 20% de anormalidades não devem ser usadas em programas de inseminação artificial. O objetivo do trabalho foi conferir se o vírus da Artrite Encefalite Caprina (CAEV) interfere na patologia espermática e qual seria o melhor diluente a ser utilizado em futuros experimentos com acréscimo de antivirais ao diluente. O 650 $aGoats 650 $aSpermatozoa 650 $aCaprino 650 $aConservação 650 $aEspermatozóide 650 $aSêmen 653 $aCAEV 653 $aCaprine arthritis encephalit virus 653 $aDefesa antiviral 653 $aDiluente 653 $aMeio Essencial Mínimo 653 $aMEM 653 $aMorfologia espermática 653 $aParâmetros espermáticos 653 $aSemen preservation 653 $aTris gema 653 $aVírus da Artrite Encefalite Caprina 700 1 $aPEIXOTO, R. M. 700 1 $aARAÚJO, J. F. 700 1 $aSILVA, A. F. 700 1 $aSANTOS, D. O. 700 1 $aANDRIOLI, A.
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