Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
23/03/2011 |
Data da última atualização: |
23/03/2011 |
Autoria: |
TELLES, M. P. C.; PEIXOTO, F. P.; LIMA, J. S.; RESENDE, L. V.; VIANELLO, R. P.; WALTER, M. E. M. T.; COLLEVATTI, R. G. |
Afiliação: |
M. P. C. TELLES, UFG; F. P. PEIXOTO, UFG; J. S. LIMA, UFG; L. V. RESENDE, UFG; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; M. E. M. T. WALTER, UNB; R. G. COLLEVATTI, UFG. |
Título: |
Development of microsatellite markers for the endangered neotropical tree species Tibouchina papyrus (Melastomataceae). |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 10, n. 1, p. 321-325, 2011. |
Idioma: |
Inglês |
Conteúdo: |
We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in ?cerrado rupestre? from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic. Among the polymorphic loci, expected heterozygosities ranged from 0.161 to 0.714. Combined paternity exclusion probability was 0.957 and combined genetic identity (0.051) was high for studies on parentage. Tibouchina papyrus is a rare and endemic tree species of outcrop quartzite and sandstone soils, with highly isolated populations, which may have lead to the low degree of polymorphism that we detected. Also, motifs of most loci are larger than dinucleotide, which typically display lower levels of polymorphism. |
Palavras-Chave: |
Pau-papel; Tibouchina papyrus. |
Thesagro: |
Árvore; Cerrado. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30810/1/gmr.pdf
|
Marc: |
LEADER 01815naa a2200241 a 4500 001 1882313 005 2011-03-23 008 2011 bl uuuu u00u1 u #d 100 1 $aTELLES, M. P. C. 245 $aDevelopment of microsatellite markers for the endangered neotropical tree species Tibouchina papyrus (Melastomataceae).$h[electronic resource] 260 $c2011 520 $aWe isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in ?cerrado rupestre? from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic. Among the polymorphic loci, expected heterozygosities ranged from 0.161 to 0.714. Combined paternity exclusion probability was 0.957 and combined genetic identity (0.051) was high for studies on parentage. Tibouchina papyrus is a rare and endemic tree species of outcrop quartzite and sandstone soils, with highly isolated populations, which may have lead to the low degree of polymorphism that we detected. Also, motifs of most loci are larger than dinucleotide, which typically display lower levels of polymorphism. 650 $aÁrvore 650 $aCerrado 653 $aPau-papel 653 $aTibouchina papyrus 700 1 $aPEIXOTO, F. P. 700 1 $aLIMA, J. S. 700 1 $aRESENDE, L. V. 700 1 $aVIANELLO, R. P. 700 1 $aWALTER, M. E. M. T. 700 1 $aCOLLEVATTI, R. G. 773 $tGenetics and Molecular Research$gv. 10, n. 1, p. 321-325, 2011.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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