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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/11/2014 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTANA-VIEIRA, D. D. S.; MILORI, D. M. B. P.; BOAS, P. R. V.; SILVA, M. F. e; SANTOS, M. G.; GAIOTTO, F. A.; SOARES FILHO, W. dos S.; GESTEIRA, A. da S. |
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Afiliação: |
DAYSE DRIELLY SOUZA SANTANA-VIEIRA, UESC; DEBORA MARCONDES BASTOS P MILORI, CNPDIA; PAULINO RIBEIRO VILLAS BOAS, CNPDIA; MARINA FRANÇA E SILVA; MAGNO GUIMARÃES SANTOS; FERNANDA AMATO GAIOTTO, UESC; WALTER DOS SANTOS SOARES FILHO, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Rapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Advances in Bioscience and Biotechnology, v.5, p. 903-914, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing clearly Tropical from Maravilha, but not Comum from Florida selections. In this way the results suggest that LIFS may be a sound tool for helping the identification of closely Citrus varieties. MenosThe differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing... Mostrar Tudo |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02359naa a2200217 a 4500
001 1999430
005 2023-05-26
008 2014 bl uuuu u00u1 u #d
100 1 $aSANTANA-VIEIRA, D. D. S.
245 $aRapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy.$h[electronic resource]
260 $c2014
520 $aThe differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing clearly Tropical from Maravilha, but not Comum from Florida selections. In this way the results suggest that LIFS may be a sound tool for helping the identification of closely Citrus varieties.
650 $aCitrus
700 1 $aMILORI, D. M. B. P.
700 1 $aBOAS, P. R. V.
700 1 $aSILVA, M. F. e
700 1 $aSANTOS, M. G.
700 1 $aGAIOTTO, F. A.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGESTEIRA, A. da S.
773 $tAdvances in Bioscience and Biotechnology$gv.5, p. 903-914, 2014.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 10 | |
| 3. |  | RODRIGUES, J. C. M.; JOHNSON, S. D.; KOLTUNOW, A. M. Epigenetic regulation of autonomous seed formation in Hieracium. In: CONGRESSO INTERNACIONAL DE REPRODUÇÃO SEXUAL EM PLANTAS, 20., 2008, Brasília, DF. [Anais...]. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 240 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 259). p. 125. Oral contribuition.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | KOLTUNOW, A. M.; JOHNSON, S. D.; OKADA, T.; RODRIGUES, J. C. M.; HU, Y. Developmental analysis of apomixis in mutant and wildtype hieracium. In: GATERSLEBEN RESEARCH CONFERENCE, 9.; INTERNATIONAL APOMIXIS CONFERENCE, 3., 2007, Wernigerode, Germany. Scientific programme and abstract. Gatersleben, Germany: IPK Gatersleben, 2007. p. 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | RODRIGUES, J. C. M.; TUCKER, M. R.; JOHNSON, S. D.; KOLTUNOW, A. Function of fertilization independent endosperm (FIE) during seed development in Hieracium. In: GATERSLEBEN RESEARCH CONFERENCE, 9.; INTERNATIONAL APOMIXIS CONFERENCE, 3., 2007, Wernigerode, Germany. Scientific programme and abstract. Gatersleben, Germany: IPK Gatersleben, 2007. p. 81.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | OKADA, T.; JOHNSON, S.; RODRIGES, J. C. M.; YINGKAO, H.; ITO, K.; SUZUKI, G.; MUKAI, Y.; KOLTUNOW. The initiation of apomixis in Hieracium. In: CONGRESSO INTERNACIONAL DE REPRODUÇÃO SEXUAL EM PLANTAS, 20., 2008, Brasília, DF. [Anais...]. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 240 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 259). p. 54.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | RODRIGUES, J. C. M.; GUIMARÃES, L. A.; BIOLCHI, A.; GALANTE, P.; KOLTUNOW, A. M. G.; CARNEIRO, V. T. de C. Differential expression ot two homologues of the Polycomb group genes Fertilization Independent Endosperm (FIE) and Enhancer of Zeste (EzSET) in male reproductive tissues of apomictic Brachiaria brizantha. In: INTERNATIONAL CONGRESS ON SEXUAL PLANT REPRODUCTION, 21., 2010, Bristol. Programme and abstract book... Bristol: University of Bristol, 2010. p. 69.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | OKADA, T.; HU, Y.; TUCKER, M. R.; TAYLOR, J. M.; JOHNSON, S. D.; SPRIGGS, A.; TSUCHIYA, T.; OELKERS, K.; RODRIGUES, J. C. M.; KOLTUNOW, A. M. G. Enlarging cells initiating apomixis in Hieracium praealtum transition to an embryo sac program prior to entering mitosis. Plant Physiology, v. 163, n. 1, p. 216-231, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | KOLTUNOW, A. M. G.; JOHNSON, S. D.; RODRIGUES, J. C. M.; OKADA, T.; YINGKAO, HU; TSUCHIYA, T.; WILSON, S.; FLETCHER, P.; ITO, K.; SUZUKI, G.; MUKAI, Y.; FEHRER, J.; BICKNELL, R. A. Sexual reproduction is the default mode in apomictic Hieracium subgenus Pilosella, in which two dominant loci function to enable apomixis. The Plant Journal, v. 66, p. 890-902, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 10 | |
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