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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/05/2014 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIU, J.; BRAUN, E.; QIU, W.; SHI, Y. F.; MARCELINO-GUIMARÃES, F. C.; NAVARRE, D. R.; HILL, J. H.; WHITHAM, S. A. |
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Afiliação: |
JIAN-ZHONG LIU, Zhejiang Normal University; EDWARD BRAUN, Iowa State University; WEN-LI QIU, Iowa State University; YA-FEI SHI, Zhejiang Normal University; FRANCISMAR CORRÊA MARCELINO-GUIMARÃES, CNPSO; DUROY NAVARRE, Washington State University; JOHN H. HILL, Iowa State University; STEVEN A. WHITHAM, Iowa State University. |
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Título: |
Positive and negative roles for soybean MPK6 in regulating defense responses. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Molecular Plant-Microbe Interactions, p. 1-36, Apr. 2014. |
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ISSN: |
0894-0282 |
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DOI: |
http://dx.doi.org/10.1094/MPMI-11-13-0350-R |
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Idioma: |
Inglês |
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Conteúdo: |
It has been well established that MPK6 is a positive regulator of defense responses in model plants such as Arabidopsis and tobacco. However, the functional importance of soybean MPK6 in disease resistance has not been investigated. Here, we showed that silencing of GmMPK6 in soybean using virus-induced gene silencing mediated by Bean pod mottle virus (BPMV) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. Consistent with this phenotype, expression of pathogenesis-related (PR) genes and the conjugated form of salicylic acid were significantly increased in GmMPK6-silenced plants. As expected, GmMPK6-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants, indicating a negative role of GmMPK6 in disease resistance. Interestingly, over-expression of GmMPK6, either transiently in N. benthamiana or stably in Arabidopsis, resulted in HR-like cell death. The HR-like cell death was accompanied by increased PR gene expression, suggesting that GmMPK6, like its counterpart in other plant species, also plays a positive role in cell death induction and defense response. Using bimolecular fluorescence complementation analysis, we determined that GmMKK4 might function upstream of GmMPK6 and GmMKK4 could interact with GmMPK6 independent of its phosphorylation status. Taken together, our results indicate that GmMPK6 functions as both repressor and activator in defense responses of soybean. MenosIt has been well established that MPK6 is a positive regulator of defense responses in model plants such as Arabidopsis and tobacco. However, the functional importance of soybean MPK6 in disease resistance has not been investigated. Here, we showed that silencing of GmMPK6 in soybean using virus-induced gene silencing mediated by Bean pod mottle virus (BPMV) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. Consistent with this phenotype, expression of pathogenesis-related (PR) genes and the conjugated form of salicylic acid were significantly increased in GmMPK6-silenced plants. As expected, GmMPK6-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants, indicating a negative role of GmMPK6 in disease resistance. Interestingly, over-expression of GmMPK6, either transiently in N. benthamiana or stably in Arabidopsis, resulted in HR-like cell death. The HR-like cell death was accompanied by increased PR gene expression, suggesting that GmMPK6, like its counterpart in other plant species, also plays a positive role in cell death induction and defense response. Using bimolecular fluorescence complementation analysis, we determined that GmMKK4 might function upstream of GmMPK6 and GmMKK4 could interact with GmMPK6 independent of its phosphorylation status. Taken together, our results indicate that GmMPK6 functions as both repressor and activator in defense r... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02217naa a2200241 a 4500
001 1985575
005 2017-08-05
008 2014 bl uuuu u00u1 u #d
022 $a0894-0282
024 7 $ahttp://dx.doi.org/10.1094/MPMI-11-13-0350-R$2DOI
100 1 $aLIU, J.
245 $aPositive and negative roles for soybean MPK6 in regulating defense responses.$h[electronic resource]
260 $c2014
520 $aIt has been well established that MPK6 is a positive regulator of defense responses in model plants such as Arabidopsis and tobacco. However, the functional importance of soybean MPK6 in disease resistance has not been investigated. Here, we showed that silencing of GmMPK6 in soybean using virus-induced gene silencing mediated by Bean pod mottle virus (BPMV) caused stunted growth and spontaneous cell death on the leaves, a typical phenotype of activated defense responses. Consistent with this phenotype, expression of pathogenesis-related (PR) genes and the conjugated form of salicylic acid were significantly increased in GmMPK6-silenced plants. As expected, GmMPK6-silenced plants were more resistant to downy mildew and Soybean mosaic virus compared with vector control plants, indicating a negative role of GmMPK6 in disease resistance. Interestingly, over-expression of GmMPK6, either transiently in N. benthamiana or stably in Arabidopsis, resulted in HR-like cell death. The HR-like cell death was accompanied by increased PR gene expression, suggesting that GmMPK6, like its counterpart in other plant species, also plays a positive role in cell death induction and defense response. Using bimolecular fluorescence complementation analysis, we determined that GmMKK4 might function upstream of GmMPK6 and GmMKK4 could interact with GmMPK6 independent of its phosphorylation status. Taken together, our results indicate that GmMPK6 functions as both repressor and activator in defense responses of soybean.
650 $aSoja
700 1 $aBRAUN, E.
700 1 $aQIU, W.
700 1 $aSHI, Y. F.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aNAVARRE, D. R.
700 1 $aHILL, J. H.
700 1 $aWHITHAM, S. A.
773 $tMolecular Plant-Microbe Interactions, p. 1-36, Apr. 2014.
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| 4. |  | BARBOSA FILHO, M. P. Cereais. In: FERREIRA, M. E.; CRUZ, M. C. P. da (Ed.). Micronutrientes na agricultura. Piracicaba: POTAFOS: CNPq, 1991. p. 413-444.| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
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| 5. | | BARBOSA FILHO, M. P. Calagem e adubação. In: STONE, L. F.; MOREIRA, J. A. A.; RABELO, R. R.; BIAVA, M. (Ed.). Arroz: o produtor pergunta, a Embrapa responde. Brasília: Embrapa Informação Tecnológica, 2001. p. 35-71. (Coleção 500 perguntas 500 respostas).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 19. | | BARBOSA FILHO, M. P.; FAGERIA, N. K. Calagem e adubação. In: SANTIAGO, C. M.; BRESEGHELLO, H. C. de P.; FERREIRA, C. M. (Ed.). Arroz: o produtor pergunta, a Embrapa responde. 2. ed. rev. e ampl. Brasília, DF: Embrapa, 2013. p. 37-73. (Coleção 500 perguntas, 500 respostas).| Tipo: Capítulo em Livro Técnico-Científico |
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