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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. |
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Afiliação: |
JULIANA POLISSENI, UFJF; JOÃO GABRIEL VIANA DE GRÁZIB, UFJF; MARTHA DE OLIVEIRA GUERRA, UFJF; LARISSA CABRAL MILEN, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF. |
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Título: |
Impact of the use of gonadotropins on embryonic development - a rat model. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Objective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos. MenosObjective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control e... Mostrar Tudo |
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Palavras-Chave: |
Animal model; Embryo development; Embryo grade. |
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Thesaurus Nal: |
superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02182naa a2200229 a 4500
001 1980786
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
100 1 $aPOLISSENI, J.
245 $aImpact of the use of gonadotropins on embryonic development - a rat model.$h[electronic resource]
260 $c2013
520 $aObjective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos.
650 $asuperovulation
653 $aAnimal model
653 $aEmbryo development
653 $aEmbryo grade
700 1 $aGRÁZIB, J. G. V. de
700 1 $aGUERRA, M. DE O.
700 1 $aMILEN, L. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aPETERS, V. M.
773 $tJBRA Assisted Reproduction$gv. 17, n. 1, p. 32-36, 2013.
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Embrapa Gado de Leite (CNPGL) |
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| 2. |  | GILES, A. L.; FREITAS, M. G.; ESPADA, A. L. V.; CONSTANTINO, P.; FRANCISCO, B. S.; RODRIGUES, S. B.; SAMPAIO, A. B.; FREITAS, I. F. de; BUENO, G. C. S.; LACERDA, R. C. A.; EDSON JÚNIOR, R.; SILVIUS, K.; VIEIRA, D. L. M. Brazil’s science–policy effort to monitor native vegetation restoration. Ambio, 2026. On-line first.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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