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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. |
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Afiliação: |
JULIANA POLISSENI, UFJF; JOÃO GABRIEL VIANA DE GRÁZIB, UFJF; MARTHA DE OLIVEIRA GUERRA, UFJF; LARISSA CABRAL MILEN, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF. |
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Título: |
Impact of the use of gonadotropins on embryonic development - a rat model. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Objective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos. MenosObjective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control e... Mostrar Tudo |
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Palavras-Chave: |
Animal model; Embryo development; Embryo grade. |
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Thesaurus Nal: |
superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| 2. |  | SANTOS JÚNIOR, J. B. O.; AMORIM, J. R. de A.; RESENDE, R. S.; SANTOS LIMA, M. A.; MATOS, J. D. S. de; SILVA, P. S. da. Caracterização hidrodinâmica de poços tubulares na bacia hidrográfica do rio Sergipe. In: CONGRESSO NACIONAL DE IRRIGAÇÃO E DRENAGEM, 17., 2007, Mossoró. Agricultura no semi-árido: anais. Mossoró: [s.n.], 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 6. | | CARVALHO, H. W. L. de; CARDOSO, M. J.; SANTOS, M. X. dos; TABOSA, J. N.; AMORIM, J. R. de A.; CARVALHO, L. M. de; MICHEREFF FILHO, M.; SOUZA, E. M. de. Adaptabilidade e estabilidade de cultivares de milho no Nordeste brasileiro no ano agrícola de 2004. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 3., 2005, Gramado. Anais... Passo Fundo: Embrapa Trigo: SBMP, 2005.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| 7. | | CARVALHO, H. W. L. de; CARDOSO, M. J.; LEAL, M. de L. da S.; AMORIM, J. R. de A.; CARVALHO, L. M. de; SANTOS, M. X. dos; SANTOS, D. M. dos S.; TABOSA, J. N.; LIRA, M. A.; SOUZA E. M. de. Comportamento de cultivares de milho no Nordeste Brasileiro no ano agrícola de 2004. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 3., 2005, Gramado. Anais... Passo Fundo: Embrapa Trigo: SBMP, 2005.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| 8. | | CARVALHO, H. W. L. de; CARDOSO, M. J.; LEAL, M. de L. da S.; AMORIM, J. R. de A.; CARVALHO, L. M. de; MICHEREFF FILHO, M.; SANTOS, M. X. dos; SANTOS, D. M. dos; TABOSA, J. N.; LIRA, M. A.; SOUZA, E. M. de. Comportamento de híbridos de milho no Nordeste Brasileiro no ano agrícola de 2004. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 3., 2005, Gramado. Anais... Passo Fundo: Embrapa Trigo: SBMP, 2005.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| Registros recuperados : 8 | |
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