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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
20/02/2014 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, A. V.; DEFAVERI, A. C. A. e; BIZZO, H. R.; GIL, R. A. da S. S.; SATO, A. |
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Afiliação: |
ALINE VIEIRA SANTOS, UFRJ; ANNA CARINA ANTUNES E DEFAVERI, UFRJ; HUMBERTO RIBEIRO BIZZO, CTAA; ROSANE AGUIAR DA SILVA SAN GIL, UFRJ; ALICE SATO, UNIRIO. |
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Título: |
In vitro propagation, histochemistry, and analysis of essential oil from conventionally propagated and in vitro-propagated plants of Varronia curassavica Jacq. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, v. 49, n. 4, p. 405-413, Aug. 2013. |
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DOI: |
10.1007/s11627-013-9528-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Varronia curassavica is cultivated for the production of an essential oil useful in the pharmaceutical industry for its strong anti-inflammatory effect. Despite a growing demand, only a few studies have evaluated alternative sources of obtaining plantlets or ways to increase the yield of essential oil from this species. Therefore, this study aimed to optimize the in vitro multiplication rate and analyze the histochemistry and sesquiterpene production potential of conventionally propagated V. curassavica plants, in vitro shoots, and acclimatized plants derived from in vitro shoots. For axillary bud proliferation, Murashige and Skoog medium was supplemented with 6-benzyladenine and thidiazuron alone or in combination with naphthalene acetic acid. Axillary bud proliferation was obtained from culture of nodal or apical segments on medium containing half-strength Murashige and Skoog salts without growth regulators. After 35 d of culture, an average of five buds developed per explant. Elongation and rooting of shoots also occurred in this medium. After the transfer of rooted plants to ex vitro conditions, 100% of the plantlets survived. Histochemical analysis of leaf tissue showed the presence of lipids, acidic lipids, essential oil, phenols, and flavonoids. The essential oils from conventionally propagated and acclimatized plants were extracted by hydrodistillation and analyzed using gas chromatography. The essential oil from acclimatized plants had a similar profile to that from ex vitro plants, but with a higher concentration of the anti-inflammatory compound alpha-humulene. MenosVarronia curassavica is cultivated for the production of an essential oil useful in the pharmaceutical industry for its strong anti-inflammatory effect. Despite a growing demand, only a few studies have evaluated alternative sources of obtaining plantlets or ways to increase the yield of essential oil from this species. Therefore, this study aimed to optimize the in vitro multiplication rate and analyze the histochemistry and sesquiterpene production potential of conventionally propagated V. curassavica plants, in vitro shoots, and acclimatized plants derived from in vitro shoots. For axillary bud proliferation, Murashige and Skoog medium was supplemented with 6-benzyladenine and thidiazuron alone or in combination with naphthalene acetic acid. Axillary bud proliferation was obtained from culture of nodal or apical segments on medium containing half-strength Murashige and Skoog salts without growth regulators. After 35 d of culture, an average of five buds developed per explant. Elongation and rooting of shoots also occurred in this medium. After the transfer of rooted plants to ex vitro conditions, 100% of the plantlets survived. Histochemical analysis of leaf tissue showed the presence of lipids, acidic lipids, essential oil, phenols, and flavonoids. The essential oils from conventionally propagated and acclimatized plants were extracted by hydrodistillation and analyzed using gas chromatography. The essential oil from acclimatized plants had a similar profile to that from... Mostrar Tudo |
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Palavras-Chave: |
Alfa-humuleno; Cordia verbenacea; Gemas axilares; Multiplicação in vitro. |
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Thesaurus Nal: |
Cordiaceae. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 1980680
005 2016-02-22
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1007/s11627-013-9528-6$2DOI
100 1 $aSANTOS, A. V.
245 $aIn vitro propagation, histochemistry, and analysis of essential oil from conventionally propagated and in vitro-propagated plants of Varronia curassavica Jacq.
260 $c2013
520 $aVarronia curassavica is cultivated for the production of an essential oil useful in the pharmaceutical industry for its strong anti-inflammatory effect. Despite a growing demand, only a few studies have evaluated alternative sources of obtaining plantlets or ways to increase the yield of essential oil from this species. Therefore, this study aimed to optimize the in vitro multiplication rate and analyze the histochemistry and sesquiterpene production potential of conventionally propagated V. curassavica plants, in vitro shoots, and acclimatized plants derived from in vitro shoots. For axillary bud proliferation, Murashige and Skoog medium was supplemented with 6-benzyladenine and thidiazuron alone or in combination with naphthalene acetic acid. Axillary bud proliferation was obtained from culture of nodal or apical segments on medium containing half-strength Murashige and Skoog salts without growth regulators. After 35 d of culture, an average of five buds developed per explant. Elongation and rooting of shoots also occurred in this medium. After the transfer of rooted plants to ex vitro conditions, 100% of the plantlets survived. Histochemical analysis of leaf tissue showed the presence of lipids, acidic lipids, essential oil, phenols, and flavonoids. The essential oils from conventionally propagated and acclimatized plants were extracted by hydrodistillation and analyzed using gas chromatography. The essential oil from acclimatized plants had a similar profile to that from ex vitro plants, but with a higher concentration of the anti-inflammatory compound alpha-humulene.
650 $aCordiaceae
653 $aAlfa-humuleno
653 $aCordia verbenacea
653 $aGemas axilares
653 $aMultiplicação in vitro
700 1 $aDEFAVERI, A. C. A. e
700 1 $aBIZZO, H. R.
700 1 $aGIL, R. A. da S. S.
700 1 $aSATO, A.
773 $tIn Vitro Cellular & Developmental Biology - Plant$gv. 49, n. 4, p. 405-413, Aug. 2013.
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Embrapa Agroindústria de Alimentos (CTAA) |
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| 1. |  | CAMARGO, M. E.; PRIESNITZ FILHO, W.; SILVA, J. L. S.; MALAFAIA, G. C.; CRUZ, M. R.; MOTTA, M. E. V. Construção de cenários prospectivos em sistema agroalimentar de vacaria, Rio Grande do Sul, Brasil. Agroalimentaria, v. 20, n. 38; jan.-jun., 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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