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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
20/01/2014 |
Data da última atualização: |
24/03/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ZALESKI, S. R. M.; LAZZARI, S. M. N.; LAZZAROTTO, C. M.; PANZAVOLTA, T.; IEDE, E. T.; MARQUES, F. de A. |
Afiliação: |
Scheila R. M. Zaleski, UFPR; Sonia M. N. Lazzari, UFPR; Crisleide M. Lazzarotto, UFPR; Tiziana Panzavolta, Università degli Studi di Firenze; EDSON TADEU IEDE, CNPF; Francisco de A. Marques, UFPR. |
Título: |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Revista Brasileira de Entomologia, v. 7, n. 4, p. 405-410, Dec. 2013. |
Idioma: |
Inglês |
Conteúdo: |
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism . The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus . The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples m ay indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fs t) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among population s points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them t o respond differently to control measures MenosGenetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism . The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus . The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples m ay indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fs t) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among population s points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them t o re... Mostrar Tudo |
Palavras-Chave: |
Banded pine weevil; Genética de população; Gorgulho do pínus; Molecular marker; Praga florestal. |
Thesagro: |
Inseto; Marcador Molecular. |
Thesaurus Nal: |
Insecta; Pissodes castaneus; population genetics. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/95540/1/2013-EdsonT-RBE-Genetic.pdf
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Marc: |
LEADER 02485naa a2200301 a 4500 001 1976679 005 2015-03-24 008 2013 bl uuuu u00u1 u #d 100 1 $aZALESKI, S. R. M. 245 $aGenetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism.$h[electronic resource] 260 $c2013 520 $aGenetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism . The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus . The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the most distant groups) and genetic distances of 0.13 and 0.44 for 10 out of the 20 samples m ay indicate several founding events or multiple introductions of heterogeneous strains into Brazil. The allelic fixation index (Fs t) was 0.3851, considered high, and the number of migrants (Nm) was 0.3991, indicating low gene flow among populations. The highest genetic distances were between the population from Irani, SC and Cambará do Sul, RS and Bituruna, PR, indicating an independent founding event or a particular allelic fixation in the former location. The high genetic diversity among population s points out that the populations are genetically heterogeneous with a diverse gene pool in the surveyed areas, what makes them t o respond differently to control measures 650 $aInsecta 650 $aPissodes castaneus 650 $apopulation genetics 650 $aInseto 650 $aMarcador Molecular 653 $aBanded pine weevil 653 $aGenética de população 653 $aGorgulho do pínus 653 $aMolecular marker 653 $aPraga florestal 700 1 $aLAZZARI, S. M. N. 700 1 $aLAZZAROTTO, C. M. 700 1 $aPANZAVOLTA, T. 700 1 $aIEDE, E. T. 700 1 $aMARQUES, F. de A. 773 $tRevista Brasileira de Entomologia$gv. 7, n. 4, p. 405-410, Dec. 2013.
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Embrapa Florestas (CNPF) |
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1. | | ZALESKI, S. R. M.; LAZZARI, S. M. N.; LAZZAROTTO, C. M.; PANZAVOLTA, T.; IEDE, E. T.; MARQUES, F. de A. Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. Revista Brasileira de Entomologia, v. 7, n. 4, p. 405-410, Dec. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Florestas. |
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