| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
03/01/2014 |
|
Data da última atualização: |
27/09/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
GONÇALVES, R. M.; FIGUEIREDO, J. E. F.; PEDRO, E. S.; MEIRELLES, W. F.; LEITE JÚNIOR, R. P.; SAUER, A. V.; PACCOLA-MEIRELLES, L. D. |
|
Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS. |
|
Título: |
Etiology and Phaeosphaeria leaf spot disease of maize. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Journal of Plant Pathology, v. 95, n. 3, p. 559-569, 2013. |
|
DOI: |
10.4454/JPP.V95I3.037 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Different fungal species and the bacterium Pantoea ananatis (Pa) have been reported as etiological agents of Phaeosphaeria leaf spot (PLS) disease. This work aimed at using molecular identification of the fungi and bacteria occurring in PLS and its etiology. Genomic DNAs from (i) pool of each of the four stages of PLS lesions; (ii) bacteria and fungi isolated from lesions of natural PLS (NPLS) and artificial injuries (AI); (iii) fungi isolated from lesions obtained from plants inoculated with Pain greenhouse (GH) were used in PCR with universal primers for bacteria and fungal rRNA genes, and species-specific primers for Pa. Bacterial amplicons were observed at all stages of lesions and fungal amplicons in stages 3 and 4. Bacterial amplicons of pooled NPLS lesions were from Pawhile fungal amplicons were from Phaeosphaeria sp. and Phoma sp. Bacteria from NPLS, GH and AI lesions were identified as Pa, Pa and Bacillus subtilis, respectively, while the fungi were Epicoccum nigrum, Leptosphaeria sacchari, Cochliobolus geniculatus, Pithomyces chartarum, Alternaria alternata, A. ricini, Gibberella intricans, G. fujikuroi, Phaeosphaeria sp., P. avenaria, Phoma sp., Phyllosticta sp., Sarocladium strictum, Glomerella graminicola, and Cochliobolus heterostrophus. Symptoms of PLS decreased by 90% with the use of oxytetracycline in foliar treatment of maize plants in the field, and its addition to culture medium completely inhibited the growth of Pa. The results strongly show that Pa is the causal agent of PLS disease and different species of opportunistic fungi appear late in the necrotic stages of lesions caused by Pa. MenosDifferent fungal species and the bacterium Pantoea ananatis (Pa) have been reported as etiological agents of Phaeosphaeria leaf spot (PLS) disease. This work aimed at using molecular identification of the fungi and bacteria occurring in PLS and its etiology. Genomic DNAs from (i) pool of each of the four stages of PLS lesions; (ii) bacteria and fungi isolated from lesions of natural PLS (NPLS) and artificial injuries (AI); (iii) fungi isolated from lesions obtained from plants inoculated with Pain greenhouse (GH) were used in PCR with universal primers for bacteria and fungal rRNA genes, and species-specific primers for Pa. Bacterial amplicons were observed at all stages of lesions and fungal amplicons in stages 3 and 4. Bacterial amplicons of pooled NPLS lesions were from Pawhile fungal amplicons were from Phaeosphaeria sp. and Phoma sp. Bacteria from NPLS, GH and AI lesions were identified as Pa, Pa and Bacillus subtilis, respectively, while the fungi were Epicoccum nigrum, Leptosphaeria sacchari, Cochliobolus geniculatus, Pithomyces chartarum, Alternaria alternata, A. ricini, Gibberella intricans, G. fujikuroi, Phaeosphaeria sp., P. avenaria, Phoma sp., Phyllosticta sp., Sarocladium strictum, Glomerella graminicola, and Cochliobolus heterostrophus. Symptoms of PLS decreased by 90% with the use of oxytetracycline in foliar treatment of maize plants in the field, and its addition to culture medium completely inhibited the growth of Pa. The results strongly show that Pa is t... Mostrar Tudo |
|
Palavras-Chave: |
Plant diseases. |
|
Thesagro: |
Doença de planta; Milho; Zea mays. |
|
Thesaurus Nal: |
Foliar diseases; Pantoea ananatis; Polymerase chain reaction. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
null
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 4 | |
| 1. |  | AMABILE, R. F.; FIDELIS, L. R. G.; INÁCIO, A. A. do N.; SOUSA, D. A.; MONTEIRO, V. A.; PEREIRA, V. C.; GOMES, A. C. Estudo do efeito de épocas de semeadura e influência do posicionamento dos grãos em espiga na germinação de sementes de cevada (Hordeum vulgare L.) na região do cerrado. In: REUNIÃO NACIONAL DE PESQUISA DE CEVADA, 27., 2009, Passo Fundo. Anais... Passo Fundo: Embrapa Trigo, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Cerrados. |
|   |
| 2. | | AMABILE, R. F.; FIDELIS, L. R. G.; INÁCIO, A. A. do N.; SOUSA, D. A.; MONTEIRO, V. A.; GOMES, A. C. Estudo do efeito de épocas de semeadura e influência do posicionamento dos grãos em espiga na germinação de sementes de cevada (Hordeum vulgare) na região do cerrado. In: REUNIÃO NACIONAL DE PESQUISA DE CEVADA, 26., 2007, Passo Fundo. Anais. Passo Fundo: Embrapa Trigo, 2007. p. 342-350. (Embrapa Trigo. Documentos, 76).| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Cerrados. |
| |
| 3. | | SOUSA, D. A.; CASCON, C. M.; MELLO, M. F. V.; LEITE, J. S.; MEDEIROS, M. A.; FONSECA, A. B. M.; FERREIRA, A. M. R. Helicobacter spp. in domestic cats: identification and relationship with anatomical and histopathological gastric changes in animals of blood group A. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1467-1473, dezembro 2017. Título em português: Helicobacter spp. em gatos domésticos: identificação e relação com alterações gástricas anatômicas e histopatológicas em animais de sangue tipo A.| Biblioteca(s): Embrapa Unidades Centrais. |
|  |
| 4. | | NETO, S. M.; COSTA, F. T.; ALMEIDA, R. G.; SOUSA, D. A.; BLOCH JÚNIOR, C. VASCONCELOS, I. M.; OLIVEIRA, J. T.; FRANCO, O. L. Isolation and molecular characterization of a novel 2S albumin with antimicrobial properties from Sesamum indicum L. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 4 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
