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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
17/12/2013 |
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Data da última atualização: |
17/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, S. R. da; SANTOS, C. A. F. |
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Afiliação: |
S. R. da COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, BA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Allelic database and divergence among Psidium accessions by using microsatellite markers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 12, n. 4, p. 6802-6812, 2013. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species. MenosThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variabili... Mostrar Tudo |
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Palavras-Chave: |
Banco ativo de germoplasma; Dendrograma; Guava; Marcadores microssatélites; Melhoramento genético; Variabilidade genética. |
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Thesagro: |
Goiaba. |
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Thesaurus Nal: |
Breeding; Psidium. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/974120/1/Carlos2.pdf
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Marc: |
LEADER 02480naa a2200253 a 4500
001 1974120
005 2013-12-17
008 2013 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aCOSTA, S. R. da
245 $aAllelic database and divergence among Psidium accessions by using microsatellite markers.$h[electronic resource]
260 $c2013
520 $aThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species.
650 $aBreeding
650 $aPsidium
650 $aGoiaba
653 $aBanco ativo de germoplasma
653 $aDendrograma
653 $aGuava
653 $aMarcadores microssatélites
653 $aMelhoramento genético
653 $aVariabilidade genética
700 1 $aSANTOS, C. A. F.
773 $tGenetics and Molecular Research$gv. 12, n. 4, p. 6802-6812, 2013.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
03/07/2019 |
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Data da última atualização: |
01/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
QUADROS, C. da C. de; LIMA, K. O.; BUENO, C. H. L.; FOGACA, F. H. dos S.; ROCHA, M. da; PRENTICE, C. |
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Afiliação: |
Camila Da Costa De Quadros, UFRS; Karina Oliveira Lima, UFRS; Caio Hendrix Luz Bueno, UFRS; FABIOLA HELENA DOS SANTOS FOGACA, CTAA; Meritaine da Rocha, UFRS; Carlos Prentice, UFRS. |
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Título: |
Evaluation of the Antioxidant and Antimicrobial Activity of Protein Hydrolysates and Peptide Fractions Derived from Colossoma macropomum and Their Effect on Ground Beef Lipid Oxidation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Aquatic Food Product Technology, 2019. p. 1-12. |
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ISSN: |
1547-0636 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to obtain protein hydrolysate from the mechanically separated meat of blackfin pacu to evaluate the influence by ultrafiltration in the antioxidant and antimicrobial activities of the peptidefractions obtained and to apply in ground beef to evaluate the lipid stability. The enzymatic hydrolysis was performed using the enzyme Protamex (pH 7.0, 60°C) for 240 min. The protein hydrolysate was fractionated by ultrafiltration. Then, the antioxidant capacity of the protein hydrolysate and the peptide fractions were evaluated in vitro by the methods of 2,2?-azinobis (3-ethylbenzothiazoline sulfonic acid) radical capture, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay, reducing power, and hydroxyl radical scavenging activity. The antimicrobial activity of the samples was evaluated by disc-diffusion against Staphylococcus aureus and Escherichia coli. After evaluation, the peptide fractions did not present higher bioactivities than that shown for the hydrolysate. The protein hydrolysate was applied to ground beef, where the substances reactive to thiobarbituric acid and color were evaluated during 7 days of storage at 4°C. Lipid oxidation was reduced up to 60.9% and there was no modification of the natural coloration. Thus, the protein hydrolysate can be used as an alternative source of antioxidant for the preservation of refrigerated meats. |
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Palavras-Chave: |
Bioactivity; Mechanically separated meat of fish; Protamex. |
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Thesaurus NAL: |
Enzymatic hydrolysis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02214naa a2200241 a 4500
001 2110337
005 2019-10-01
008 2019 bl uuuu u00u1 u #d
022 $a1547-0636
100 1 $aQUADROS, C. da C. de
245 $aEvaluation of the Antioxidant and Antimicrobial Activity of Protein Hydrolysates and Peptide Fractions Derived from Colossoma macropomum and Their Effect on Ground Beef Lipid Oxidation.$h[electronic resource]
260 $c2019
520 $aThe objective of this study was to obtain protein hydrolysate from the mechanically separated meat of blackfin pacu to evaluate the influence by ultrafiltration in the antioxidant and antimicrobial activities of the peptidefractions obtained and to apply in ground beef to evaluate the lipid stability. The enzymatic hydrolysis was performed using the enzyme Protamex (pH 7.0, 60°C) for 240 min. The protein hydrolysate was fractionated by ultrafiltration. Then, the antioxidant capacity of the protein hydrolysate and the peptide fractions were evaluated in vitro by the methods of 2,2?-azinobis (3-ethylbenzothiazoline sulfonic acid) radical capture, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay, reducing power, and hydroxyl radical scavenging activity. The antimicrobial activity of the samples was evaluated by disc-diffusion against Staphylococcus aureus and Escherichia coli. After evaluation, the peptide fractions did not present higher bioactivities than that shown for the hydrolysate. The protein hydrolysate was applied to ground beef, where the substances reactive to thiobarbituric acid and color were evaluated during 7 days of storage at 4°C. Lipid oxidation was reduced up to 60.9% and there was no modification of the natural coloration. Thus, the protein hydrolysate can be used as an alternative source of antioxidant for the preservation of refrigerated meats.
650 $aEnzymatic hydrolysis
653 $aBioactivity
653 $aMechanically separated meat of fish
653 $aProtamex
700 1 $aLIMA, K. O.
700 1 $aBUENO, C. H. L.
700 1 $aFOGACA, F. H. dos S.
700 1 $aROCHA, M. da
700 1 $aPRENTICE, C.
773 $tJournal of Aquatic Food Product Technology, 2019. p. 1-12.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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