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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
23/10/2013 |
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Data da última atualização: |
23/10/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROCHA-FRIGONI, N. A. S.; LEÃO, B. C. S.; NOGUEIRA, E.; ACCORSI, M. F.; MINGOTI, G. Z. |
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Afiliação: |
NATHÁLIA A. S. ROCHA-FRIGONI, UNESP; BEATRIZ C. S. LEÃO, UNESP; ERIKLIS NOGUEIRA, CPAP; MÔNICA F. ACCORSI, UNESP; GISELE Z. MINGOTI, UNESP. |
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Título: |
Reduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development - http://dx.doi.org/10.1071/RD12354 |
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ISSN: |
1031-3613 |
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Idioma: |
Inglês |
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Conteúdo: |
The effects of intracellular (cysteine and b-mercaptoethanol) and extracellular (catalase) antioxidant supplementation at different times during in vitro production (IVM and/or in vitro culture (IVC)) on bovine embryo development, intracellular reactive oxygen species (ROS) levels, apoptosis and re-expansion rates after a vitrification?thawing process were examined. Blastocyst frequencies were not affected by either antioxidant supplementation (40.5%? 56.4%) or the timing of supplementation (41.7%?55.4%) compared with control (48.7%; P.0.05). Similarly, antioxidants and the moment of supplementation did not affect (P.0.05) the total number of blastomeres (86.2?90.5 and 84.4?90.5, respectively) compared with control (85.7). However, the percentage of apoptotic cells was reduced (P,0.05) in groups supplemented during IVM (1.7%), IVC (2.0%) or both (1.8%) compared with control (4.3%). Intracellular ROS levels measured in Day 7 blastocysts were reduced (P,0.05) in all groups (0.60?0.78), with the exception of the group supplemented with b-mercaptoethanol during IVC (0.88), which did not differ (P.0.05) from that in the control group (1.00). Re-expansion rates were not affected (P.0.05) by the treatments (50.0%?93.0%). In conclusion, antioxidant supplementation during IVM and/or IVC reduces intracellular ROS and the rate of apoptosis; however, supplementation does not increase embryonic development and survival after vitrification. |
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Palavras-Chave: |
Redox status. |
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Thesaurus Nal: |
oxidative stress. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02166naa a2200205 a 4500
001 1969180
005 2013-10-23
008 2013 bl uuuu u00u1 u #d
022 $a1031-3613
100 1 $aROCHA-FRIGONI, N. A. S.
245 $aReduced levels of intracellular reactive oxygen species and apoptotic status are not correlated with increases in cryotolerance of bovine embryos produced in vitro in the presence of antioxidants.
260 $c2013
520 $aThe effects of intracellular (cysteine and b-mercaptoethanol) and extracellular (catalase) antioxidant supplementation at different times during in vitro production (IVM and/or in vitro culture (IVC)) on bovine embryo development, intracellular reactive oxygen species (ROS) levels, apoptosis and re-expansion rates after a vitrification?thawing process were examined. Blastocyst frequencies were not affected by either antioxidant supplementation (40.5%? 56.4%) or the timing of supplementation (41.7%?55.4%) compared with control (48.7%; P.0.05). Similarly, antioxidants and the moment of supplementation did not affect (P.0.05) the total number of blastomeres (86.2?90.5 and 84.4?90.5, respectively) compared with control (85.7). However, the percentage of apoptotic cells was reduced (P,0.05) in groups supplemented during IVM (1.7%), IVC (2.0%) or both (1.8%) compared with control (4.3%). Intracellular ROS levels measured in Day 7 blastocysts were reduced (P,0.05) in all groups (0.60?0.78), with the exception of the group supplemented with b-mercaptoethanol during IVC (0.88), which did not differ (P.0.05) from that in the control group (1.00). Re-expansion rates were not affected (P.0.05) by the treatments (50.0%?93.0%). In conclusion, antioxidant supplementation during IVM and/or IVC reduces intracellular ROS and the rate of apoptosis; however, supplementation does not increase embryonic development and survival after vitrification.
650 $aoxidative stress
653 $aRedox status
700 1 $aLEÃO, B. C. S.
700 1 $aNOGUEIRA, E.
700 1 $aACCORSI, M. F.
700 1 $aMINGOTI, G. Z.
773 $tReproduction, Fertility and Development - http://dx.doi.org/10.1071/RD12354
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