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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
14/02/2013 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BITAR, M.; DRUMMOND, M. G.; COSTA, M. G. S.; LOBO, F. P.; CALZAVARA-SILVA, C. E.; BISCH, P. M.; MACHADO, C. R.; MACEDO, A. M.; PIERCE, R. J.; FRANCO, G. R. |
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Afiliação: |
MAINÁ BITAR, UFRJ, UFMG; MARCELA GONÇALVES DRUMMOND, UFMG, Univ. Lille Nord de France; MAURICIO GARCIA SOUZA COSTA, UFRJ; FRANCISCO PEREIRA LOBO, CNPTIA; CARLOS EDUARDO CALZAVARA-SILVA, Fiocruz; PAULO MASCARELLO BISCH, UFRJ; CARLOS RENATO MACHADO, UFMG; ANDRÉA MARA MACEDO, UFMG; RAYMOND J. PIERCE, Univ. Lille Nord de France; GLÓRIA REGINA FRANCO, UFMG. |
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Título: |
Modeling the zing finger protein SmZF1 from Schistosoma mansoni: Insights into DNA binding and gene regulation. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Molecular Graphics and Modelling, New York, v. 39, p. 29-38, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Zinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites revealed 415 genes hypothetically under SmZF1 control. Using an automatic annotation and GO assignment approach, we found that the majority of those genes code for proteins involved in developmental processes. Taken together, these results present a consistent base to the structural and functional characterization of SmZF1. MenosZinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites reve... Mostrar Tudo |
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Palavras-Chave: |
Ligação de DNA; Regulação gênica. |
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Thesagro: |
Schistosoma Mansoni. |
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Thesaurus Nal: |
DNA-binding proteins; Gene expression regulation; Zinc finger motif. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02751naa a2200301 a 4500
001 1949183
005 2020-01-08
008 2013 bl uuuu u00u1 u #d
100 1 $aBITAR, M.
245 $aModeling the zing finger protein SmZF1 from Schistosoma mansoni$bInsights into DNA binding and gene regulation.$h[electronic resource]
260 $c2013
520 $aZinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites revealed 415 genes hypothetically under SmZF1 control. Using an automatic annotation and GO assignment approach, we found that the majority of those genes code for proteins involved in developmental processes. Taken together, these results present a consistent base to the structural and functional characterization of SmZF1.
650 $aDNA-binding proteins
650 $aGene expression regulation
650 $aZinc finger motif
650 $aSchistosoma Mansoni
653 $aLigação de DNA
653 $aRegulação gênica
700 1 $aDRUMMOND, M. G.
700 1 $aCOSTA, M. G. S.
700 1 $aLOBO, F. P.
700 1 $aCALZAVARA-SILVA, C. E.
700 1 $aBISCH, P. M.
700 1 $aMACHADO, C. R.
700 1 $aMACEDO, A. M.
700 1 $aPIERCE, R. J.
700 1 $aFRANCO, G. R.
773 $tJournal of Molecular Graphics and Modelling, New York$gv. 39, p. 29-38, 2013.
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Embrapa Agricultura Digital (CNPTIA) |
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| Registros recuperados : 32 | |
| 1. |  | RESTLE, J.; VAZ, F. N. Aspectos quantitativos da carcaça de machos Hereford, inteiros e castrados, abatidos aos quatorze meses. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 32, n. 10, p. 1091-1095, out. 1997. Título em inglês: Carcass quantitative traits of intact and castrated Hereford males, slaughtered at foursteen months.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | RESTLE, J.; KEPLIN, L. A. da S.; VAZ, F. N. Características quantitativas da carcaça de novilhos Charolês, abatidos com diferentes pesos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 32, n. 8, p. 851-856, ago. 1997. Título em inglês: Quantitative carcass traits of Charolais steers, slaughtered with different weights.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | RESTLE, J.; BRONDANI, I. L.; FLORES, J. L. C.; VAZ, F. N.; ANTUNES, C. Desempenho de genótipos de novilhos para abate aos catorze meses, gerados por fêmeas de dois anos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 11, p. 2123-28, nov. 1999 Título em inglês: Performance of genotypes of steers for slaughter at foursteen months, produced by females of two years.b| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | RESTLE, J.; VAZ, F. N.; BRONDANI, I. L.; GONÇALVES, J. M.; ANDREATTA, E. Estudo da carcaça de machos Bradford desmamados aos 72 ou 210 dias, abatidos aos catorze meses. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 11, p. 2137-44, nov. 1999 Título em inglês: Carcass study of braford males weaned at 72 or 2010 days, slaughtered at fourteen months.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | LUPATINI, G. C.; RESTLE, J.; CERETTA, M.; MOOJEN, E. L.; BARTZ, H. R. Avaliação da mistura de aveia preta e azevém sob pastejo submetida a níveis de nitrogênio. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 33, n. 11, p. 1939-43, nov. 1998. Título em inglês: Evaluation of black oats and italian ryegrass mixture under grazing with nitrogen levels.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | EUCLIDES FILHO, K.; RESTLE, J.; OLSON, T. A.; KOGER, M.; HARGROVE, D. D. Milk consuption, feed intake, weaning weight and measures of efficiency of calves from Angus, Brown Swiss and F1 Brown Swiss x Angus cows under semi-confinament. Journal of Animal Science, Champaign, v.57, suppl. 1, p.27, 1983. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 14. | | MOOJEN, E. L.; RESTLE, J.; LUPATINI, G. C.; MORAES, A. G. de. Produção animal em pastagem de milheto sob diferentes níveis de nitrogênio. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 11, p. 2145-49, nov. 1999 Título em inglês: Animal production in pearl millet pasture under different nitrogen levels.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 15. | | RESTLE, J.; EUCLIDES FILHO, K.; HARGROVE, D. D.; ROGER, M.; OLSON, T. A. Desempenho em confinamento de Novilhos Angus, Pardo Suico, f1, f2 e retrocruzados. In: REUNIAO DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 21.,1984, Belo Horizonte. Anais... Belo Horizonte : SBZ, 1984. p.115. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | LUPATINI, G. C.; MOOJEN, E. L.; RESTLE, J.; SILVA, J. H. S. da. Resposta do milheto (Pennisetum americanum(L.) Leeke) sob pastejo a adubação nitrogenada. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 31, n. 10, p. 715-720, out. 1996. Título em inglês: Evaluation of pearl millet (Pennisetum americanum(L.) Leeke) under grazing with nitrogen levels.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | FERNANDES, T. A.; CERDÓTES, L.; VAZ, R. Z.; RESTLE, J.; FERREIRA, O. G. L. Relationship between heterosis, weight gain, and body measurements of Nellore and Charolais calves. Pesquisa Agropecuária Brasileira, v. 55, e01821, 2020. Título em português: Relação entre heterose, ganho de peso e medidas corporais de bezerros Nelore e Charolês.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 18. | | RESTLE, J.; VAZ, F. N.; PASCOAL, L. L.; SENNA, D. B. de; VAZ, R. Z.; FEIJO, G. L. D. Efeito do desmame precoce na carcaça de novilhos terminados em pastagem e abatidos aos 24 meses. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 11, p. 2129-36, nov. 1999 Título em inglês: Eartly weaning effect of carcass of steers finished on pasture and slaughtered at twenty-four months.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 19. | | VAZ, F. N.; RESTLE, J.; EIFERT, E. da C.; BRONDANI, I. L.; VAZ, R. Z.; ARGENTA, F. M. Efeitos da altura de colheita da silagem de milho e do nível de concentrado sobre as características da carcaça e da carne de novilhos superjovens. Ciência Animal Brasileira, Goiânia, v. 11, n. 2, p. 315-325, abr./jun. 2010| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 32 | |
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