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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Instrumentação. |
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Data corrente: |
08/02/2012 |
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Data da última atualização: |
18/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELABONA, P. da S.; PIROTA, R. D. P. B.; CODIMA, C. A.; TREMACOLDI, C. R.; RODRIGUES, A.; FARINAS, C. S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Using amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Biomass and Bioenergy,Oxford, v. 37, p. 243-250, 2012. |
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DOI: |
10.1016/j.biombioe.2011.12.006 |
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Idioma: |
Inglês |
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Conteúdo: |
The successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, b-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g1 for CMCase, 5.0 FPU g1 for FPAse, 105.82 IU g1 for b-glucosidase and 1055.62 IU g1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 C or pH 3e3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion. |
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Palavras-Chave: |
Agro-industrial residues; Amazon forest; Floresta amazônica; Resíduo agroindustrial. |
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Thesagro: |
Enzima; Fungo. |
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Thesaurus Nal: |
Bioethanol; Cellulases; Enzymes; Solid state fermentation. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal
S Ciências Biológicas |
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Marc: |
LEADER 02306naa a2200313 a 4500
001 1940885
005 2018-09-18
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biombioe.2011.12.006$2DOI
100 1 $aDELABONA, P. da S.
245 $aUsing amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes.$h[electronic resource]
260 $c2012
520 $aThe successful strategy to produce cellulolytic enzymes includes both microorganism selection and improved fermentation process conditions. This work describes the isolation, screening and selection of biomass-degrading fungi species from the Amazon forest and analyzes the enzymatic complex produced by a selected strain of Aspergillus fumigatus cultivated using different agro-industrial residues (wheat bran, sugarcane bagasse, soybean bran, and orange peel) as substrate in solid state fermentation (SSF). The profile of endoglucanase (CMCase), FPase, b-glucosidase and xylanase enzymatic activities obtained during 120 h of cultivation is presented. Enzyme activities up 160.1 IU g1 for CMCase, 5.0 FPU g1 for FPAse, 105.82 IU g1 for b-glucosidase and 1055.62 IU g1 for xylanase were achieved. The enzymatic extract with higher CMCase activity was used to run a zymogram analysis that showed 3 bands of endoglucanase activity. Characterization studies of this extract showed that the CMCase was most active at either 65 C or pH 3e3.5, indicating that this microorganism produces a thermophilic and acid endoglucanase. These data demonstrate that the fungal isolates from the Amazon forest are a potential source of cellulases and xylanases, providing support to further studies related to the use of these microorganisms to obtain the enzymes needed for biomass conversion.
650 $aBioethanol
650 $aCellulases
650 $aEnzymes
650 $aSolid state fermentation
650 $aEnzima
650 $aFungo
653 $aAgro-industrial residues
653 $aAmazon forest
653 $aFloresta amazônica
653 $aResíduo agroindustrial
700 1 $aPIROTA, R. D. P. B.
700 1 $aCODIMA, C. A.
700 1 $aTREMACOLDI, C. R.
700 1 $aRODRIGUES, A.
700 1 $aFARINAS, C. S.
773 $tBiomass and Bioenergy,Oxford$gv. 37, p. 243-250, 2012.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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