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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/11/2012 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. |
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Afiliação: |
PIERRE MARRACCINI, Cenargen, CIRAD; FELIPE VINECKY, Cenargen; GABRIEL S. C. ALVES, Cenargen; HUMBERTO J. O. RAMOS, IAPAR; SONIA ELBELT, Cenargen, CIRAD; NATALIA G. VIEIRA, Cenargen; FERNANDA A. CARNEIRO, Cenargen; PATRICIA S. SUJII, Cenargen; JEAN C. ALEKCEVETCH, Cenargen; VÂNIA A. SILVA, UFV; FÁBIO M. DAMATTA, UFV; MARIA AMELIA GAVA FERRÃO, SAPC; THIERRY LEROY, CIRAD; DAVID POT, CIRAD; LUIZ G. E. VIEIRA, SAPC; FELIPE RODRIGUES DA SILVA, CNPTIA; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Experimental Botany, Oxford, v. 63, n. 11, p. 4191-4212, 2012. |
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DOI: |
10.1093/jxb/ers103 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora. MenosThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Differential expression; Drought acclimation; Proteomic; Proteômica; Real time PCR. |
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Thesagro: |
Café; Coffea Canephora; Genética. |
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Thesaurus Nal: |
Genetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03043naa a2200445 a 4500
001 1940133
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008 2012 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/ers103$2DOI
100 1 $aMARRACCINI, P.
245 $aDifferentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.
650 $aGenetics
650 $aCafé
650 $aCoffea Canephora
650 $aGenética
653 $aCandidate gene
653 $aDifferential expression
653 $aDrought acclimation
653 $aProteomic
653 $aProteômica
653 $aReal time PCR
700 1 $aVINECKY, F.
700 1 $aALVES, G. S. C.
700 1 $aRAMOS, H. J. O.
700 1 $aELBELT, S.
700 1 $aVIEIRA, N. G.
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aSILVA, V. A.
700 1 $aDaMATTA, F. M.
700 1 $aFERRÃO, M. A. G.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aVIEIRA, L. G. E.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
773 $tJournal of Experimental Botany, Oxford$gv. 63, n. 11, p. 4191-4212, 2012.
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Embrapa Agricultura Digital (CNPTIA) |
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| 1. |  | ARRUDA, R. O. M.; MORAES, R. de O.; MORAES, I. O.; CAPALBO, D. M. F. Bacillus thuringiensis biopesticide by solid state fermentation in an alternative bioreactor. In: ENCUENTRO LATINOAMERICANO Y DEL CARIBE DE BIOTECNOLOGIA AGRÍCOLA, 5, 2004, Boca Chica, República Dominicana. Resúmenes de poster. Boca Chica, República Dominicana: REDBIO/FAO, 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | CESNIK, R.; TEIXEIRA, M. F. S.; VERA, M. L.; MORAES, R. de O.; VIDAURRE, T. C. Uma cepa entomopatogênica de colletotrichum gloeosporioides penz, produzida por fermentação no estado sólido. In: Arquivos do Instituto Biológico, São Paulo, v. 71 (supl.): 192-194, 2004. Edição dos anais do XVII Reunião Anual do Instituto Biológico; II Congresso de Iniciação Científica em Ciências Agrárias, Biológicas e Ambientais, São Paulo/SP, 2004.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | MATALLO, M. B.; FRANCO, D. A. S.; ALMEIDA, S. D. B.; MOURA, M. A.; CERDEIRA, A. L.; QUEIROZ, S. C. do N. de; MORAES, R. de O.; DUKE, S. O.; SILVA, D. F. P. da. Absorção de glifosate por soja não transgênica a partir de exsudatos de raiz de soja RR cultivadas em sistema hidropônico. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. 1 Cd-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 8. | | MORAES, I. de O.; CAPALBO, D. M. F.; VALICENTI, F. H.; MORAES, R. de O.; MAIA, A. de H. N.; NASCIMENTO, M. L.; ARRUDA, R. O. M.; PELIZER, L. H. Case studies of Bacillus thuringiensis production and biocontrol applications. In: THANGADURAI, D.; TRIPATHI, L.; VASANTHAIAH, H. K. N.; CANTÚ, D. J. Crop improvement and biotechnology. Tamil Nadu: Bioscience, 2008. p.229-240.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 8 | |
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